Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies

Abstract Broadly neutralizing antibodies (bnAbs) are correlated with passive HIV/SHIV protection and are desirable components of a HIV protective immunity. In the current study, we have designed a sequential-immunization strategy with a panel of envelope glycoprotein (Env)-enriched virus-like partic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Teena Mohan, Zachary Berman, Sang-Moo Kang, Bao-Zhong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f3aac57d73f4e829272f39541f03f10
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2f3aac57d73f4e829272f39541f03f10
record_format	dspace
spelling	oai:doaj.org-article:2f3aac57d73f4e829272f39541f03f102021-12-02T15:09:06ZSequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies10.1038/s41598-018-25960-12045-2322https://doaj.org/article/2f3aac57d73f4e829272f39541f03f102018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25960-1https://doaj.org/toc/2045-2322Abstract Broadly neutralizing antibodies (bnAbs) are correlated with passive HIV/SHIV protection and are desirable components of a HIV protective immunity. In the current study, we have designed a sequential-immunization strategy with a panel of envelope glycoprotein (Env)-enriched virus-like particles (VLPs) from various HIV-1 clades (A-E) to elicit bnAbs with high breadth and potency of neutralization in rabbits. We have compared this regimen with repetitive immunizations of individual Env (subtype B) VLPs or a mixture of various Env VLPs. Our results demonstrate that the sequential immunization group of animals induced significantly higher IgG endpoint titers against respective HIV Env (autologous) antigen than other control groups. Animals vaccinated sequentially showed an increase in the antibody endpoint titers and IgG antibody secreting cells (ASCs) against Con-S Env protein. Sequential immunizations with various Env VLPs promoted antibody avidity indices and enhanced bnAb responses against a panel of HIV pseudotyped virions including some of the tier 3 pseudostrains. Sequential immunizations with various VLPs displaying “native-like” HIV-1 Envs elicited bnAb responses with increased breadth and potency of neutralization.Teena MohanZachary BermanSang-Moo KangBao-Zhong WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teena Mohan Zachary Berman Sang-Moo Kang Bao-Zhong Wang Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies
description	Abstract Broadly neutralizing antibodies (bnAbs) are correlated with passive HIV/SHIV protection and are desirable components of a HIV protective immunity. In the current study, we have designed a sequential-immunization strategy with a panel of envelope glycoprotein (Env)-enriched virus-like particles (VLPs) from various HIV-1 clades (A-E) to elicit bnAbs with high breadth and potency of neutralization in rabbits. We have compared this regimen with repetitive immunizations of individual Env (subtype B) VLPs or a mixture of various Env VLPs. Our results demonstrate that the sequential immunization group of animals induced significantly higher IgG endpoint titers against respective HIV Env (autologous) antigen than other control groups. Animals vaccinated sequentially showed an increase in the antibody endpoint titers and IgG antibody secreting cells (ASCs) against Con-S Env protein. Sequential immunizations with various Env VLPs promoted antibody avidity indices and enhanced bnAb responses against a panel of HIV pseudotyped virions including some of the tier 3 pseudostrains. Sequential immunizations with various VLPs displaying “native-like” HIV-1 Envs elicited bnAb responses with increased breadth and potency of neutralization.
format	article
author	Teena Mohan Zachary Berman Sang-Moo Kang Bao-Zhong Wang
author_facet	Teena Mohan Zachary Berman Sang-Moo Kang Bao-Zhong Wang
author_sort	Teena Mohan
title	Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies
title_short	Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies
title_full	Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies
title_fullStr	Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies
title_full_unstemmed	Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies
title_sort	sequential immunizations with a panel of hiv-1 env virus-like particles coach immune system to make broadly neutralizing antibodies
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/2f3aac57d73f4e829272f39541f03f10
work_keys_str_mv	AT teenamohan sequentialimmunizationswithapanelofhiv1envviruslikeparticlescoachimmunesystemtomakebroadlyneutralizingantibodies AT zacharyberman sequentialimmunizationswithapanelofhiv1envviruslikeparticlescoachimmunesystemtomakebroadlyneutralizingantibodies AT sangmookang sequentialimmunizationswithapanelofhiv1envviruslikeparticlescoachimmunesystemtomakebroadlyneutralizingantibodies AT baozhongwang sequentialimmunizationswithapanelofhiv1envviruslikeparticlescoachimmunesystemtomakebroadlyneutralizingantibodies
_version_	1718387928544051200

Sequential immunizations with a panel of HIV-1 Env virus-like particles coach immune system to make broadly neutralizing antibodies

Ejemplares similares