Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish

Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterizat...

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Autores principales:	Ludivine Raby, Pamela Völkel, Shaghayegh Hasanpour, Julien Cicero, Robert-Alain Toillon, Eric Adriaenssens, Isabelle Van Seuningen, Xuefen Le Bourhis, Pierre-Olivier Angrand
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	zebrafish epigenetics PRC2 EED polycomb repression Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2f3bf6f842ef4cf28ef29404595cd17a
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spelling	oai:doaj.org-article:2f3bf6f842ef4cf28ef29404595cd17a2021-11-25T17:11:54ZLoss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish10.3390/cells101131422073-4409https://doaj.org/article/2f3bf6f842ef4cf28ef29404595cd17a2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3142https://doaj.org/toc/2073-4409Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterization of a zebrafish line harboring a null allele of <i>eed</i>, a gene coding for an essential component of the PRC2. Homozygous <i>eed</i>-deficient mutants present a normal body plan development but display strong defects at the level of the digestive organs, such as reduced size of the pancreas, hepatic steatosis, and a loss of the intestinal structures, to die finally at around 10–12 days post fertilization. In addition, we found that PRC2 loss of function impairs neuronal differentiation in very specific and discrete areas of the brain and increases larval activity in locomotor assays. Our work highlights that zebrafish is a suited model to study human pathologies associated with PRC2 loss of function and H3K27me3 decrease.Ludivine RabyPamela VölkelShaghayegh HasanpourJulien CiceroRobert-Alain ToillonEric AdriaenssensIsabelle Van SeuningenXuefen Le BourhisPierre-Olivier AngrandMDPI AGarticlezebrafishepigeneticsPRC2EEDpolycomb repressionBiology (General)QH301-705.5ENCells, Vol 10, Iss 3142, p 3142 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zebrafish epigenetics PRC2 EED polycomb repression Biology (General) QH301-705.5
spellingShingle	zebrafish epigenetics PRC2 EED polycomb repression Biology (General) QH301-705.5 Ludivine Raby Pamela Völkel Shaghayegh Hasanpour Julien Cicero Robert-Alain Toillon Eric Adriaenssens Isabelle Van Seuningen Xuefen Le Bourhis Pierre-Olivier Angrand Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
description	Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterization of a zebrafish line harboring a null allele of <i>eed</i>, a gene coding for an essential component of the PRC2. Homozygous <i>eed</i>-deficient mutants present a normal body plan development but display strong defects at the level of the digestive organs, such as reduced size of the pancreas, hepatic steatosis, and a loss of the intestinal structures, to die finally at around 10–12 days post fertilization. In addition, we found that PRC2 loss of function impairs neuronal differentiation in very specific and discrete areas of the brain and increases larval activity in locomotor assays. Our work highlights that zebrafish is a suited model to study human pathologies associated with PRC2 loss of function and H3K27me3 decrease.
format	article
author	Ludivine Raby Pamela Völkel Shaghayegh Hasanpour Julien Cicero Robert-Alain Toillon Eric Adriaenssens Isabelle Van Seuningen Xuefen Le Bourhis Pierre-Olivier Angrand
author_facet	Ludivine Raby Pamela Völkel Shaghayegh Hasanpour Julien Cicero Robert-Alain Toillon Eric Adriaenssens Isabelle Van Seuningen Xuefen Le Bourhis Pierre-Olivier Angrand
author_sort	Ludivine Raby
title	Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_short	Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_full	Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_fullStr	Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_full_unstemmed	Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_sort	loss of polycomb repressive complex 2 function alters digestive organ homeostasis and neuronal differentiation in zebrafish
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2f3bf6f842ef4cf28ef29404595cd17a
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_version_	1718412639073206272

Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish

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