Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

Amitava Moulick,1,2 Vedran Milosavljevic,1,2 Jana Vlachova,1,2 Robert Podgajny,3 David Hynek,1,2 Pavel Kopel,1,2 Vojtech Adam1,2 1Department of Chemistry and Biochemistry, Mendel University, 2Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; 3Faculty of...

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Autores principales:	Moulick A, Milosavljevic V, Vlachova J, Podgajny R, Hynek D, Kopel P, Adam V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	quantum dots nucleobases biosensor fluorescence conjugate Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2f3d3edff8b24c58ab9c38896c8b3664
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spelling	oai:doaj.org-article:2f3d3edff8b24c58ab9c38896c8b36642021-12-02T00:54:54ZUsing CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA1178-2013https://doaj.org/article/2f3d3edff8b24c58ab9c38896c8b36642017-02-01T00:00:00Zhttps://www.dovepress.com/using-cdteznse-coreshell-quantum-dots-to-detect-dna-and-damage-to-dna-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Amitava Moulick,1,2 Vedran Milosavljevic,1,2 Jana Vlachova,1,2 Robert Podgajny,3 David Hynek,1,2 Pavel Kopel,1,2 Vojtech Adam1,2 1Department of Chemistry and Biochemistry, Mendel University, 2Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; 3Faculty of Chemistry, Jagiellonian University, Krakow, Poland Abstract: CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments. Keywords: nanoparticles, nucleobases, biosensor, fluorescence, mutationMoulick AMilosavljevic VVlachova JPodgajny RHynek DKopel PAdam VDove Medical Pressarticlequantum dotsnucleobasesbiosensorfluorescenceconjugateMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1277-1291 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	quantum dots nucleobases biosensor fluorescence conjugate Medicine (General) R5-920
spellingShingle	quantum dots nucleobases biosensor fluorescence conjugate Medicine (General) R5-920 Moulick A Milosavljevic V Vlachova J Podgajny R Hynek D Kopel P Adam V Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
description	Amitava Moulick,1,2 Vedran Milosavljevic,1,2 Jana Vlachova,1,2 Robert Podgajny,3 David Hynek,1,2 Pavel Kopel,1,2 Vojtech Adam1,2 1Department of Chemistry and Biochemistry, Mendel University, 2Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; 3Faculty of Chemistry, Jagiellonian University, Krakow, Poland Abstract: CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments. Keywords: nanoparticles, nucleobases, biosensor, fluorescence, mutation
format	article
author	Moulick A Milosavljevic V Vlachova J Podgajny R Hynek D Kopel P Adam V
author_facet	Moulick A Milosavljevic V Vlachova J Podgajny R Hynek D Kopel P Adam V
author_sort	Moulick A
title	Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
title_short	Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
title_full	Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
title_fullStr	Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
title_full_unstemmed	Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA
title_sort	using cdte/znse core/shell quantum dots to detect dna and damage to dna
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/2f3d3edff8b24c58ab9c38896c8b3664
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Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

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