Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.

<h4>Background</h4>Human mesenchymal stem cells (hMSCs) are multipotent by nature and are originally isolated from bone marrow. In light of a future application of hMSCs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacteria...

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Autores principales:	Katja Kriebel, Anne Biedermann, Bernd Kreikemeyer, Hermann Lang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f45b5892d7442c18a970b124a0b94cb
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spelling	oai:doaj.org-article:2f45b5892d7442c18a970b124a0b94cb2021-11-18T08:48:35ZAnaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.1932-620310.1371/journal.pone.0078226https://doaj.org/article/2f45b5892d7442c18a970b124a0b94cb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24223777/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Human mesenchymal stem cells (hMSCs) are multipotent by nature and are originally isolated from bone marrow. In light of a future application of hMSCs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study to gain information about the behavior of hMSC in an anaerobic system and the response in interaction with oral bacterial pathogens.<h4>Methodology/principal findings</h4>We established a model system with oral pathogenic bacterial species and eukaryotic cells cultured in anaerobic conditions. The facultative anaerobe bacteria Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were studied. Their effects on hMSCs and primary as well as permanent gingival epithelial cells (Ca9-22, HGPEC) were comparatively analyzed. We show that hMSCs cope with anoxic conditions, since 40% vital cells remain after 72 h of anaerobic culture. The Ca9-22 and HGPEC cells are significantly more sensitive to lack of oxygen. All bacterial species reveal a comparatively low adherence to and internalization into hMSCs (0.2% and 0.01% of the initial inoculum, respectively). In comparison, the Ca9-22 and HGPEC cells present better targets for bacterial adherence and internalization. The production of the pro-inflammatory chemokine IL-8 is higher in both gingival epithelial cell lines compared to hMSCs and Fusobacterium nucleatum induce a time-dependent cytokine secretion in both cell lines. Porphyromonas gingivalis is less effective in stimulating secretion of IL-8 in the co-cultivation experiments.<h4>Conclusions/significance</h4>HMSCs are suitable for use in anoxic regions of the oral cavity. The interaction with local pathogenic bacteria does not result in massive pro-inflammatory cytokine responses. The test system established in this study allowed further investigation of parameters prior to set up of oral hMSC in vivo studies.Katja KriebelAnne BiedermannBernd KreikemeyerHermann LangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e78226 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katja Kriebel Anne Biedermann Bernd Kreikemeyer Hermann Lang Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.
description	<h4>Background</h4>Human mesenchymal stem cells (hMSCs) are multipotent by nature and are originally isolated from bone marrow. In light of a future application of hMSCs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study to gain information about the behavior of hMSC in an anaerobic system and the response in interaction with oral bacterial pathogens.<h4>Methodology/principal findings</h4>We established a model system with oral pathogenic bacterial species and eukaryotic cells cultured in anaerobic conditions. The facultative anaerobe bacteria Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were studied. Their effects on hMSCs and primary as well as permanent gingival epithelial cells (Ca9-22, HGPEC) were comparatively analyzed. We show that hMSCs cope with anoxic conditions, since 40% vital cells remain after 72 h of anaerobic culture. The Ca9-22 and HGPEC cells are significantly more sensitive to lack of oxygen. All bacterial species reveal a comparatively low adherence to and internalization into hMSCs (0.2% and 0.01% of the initial inoculum, respectively). In comparison, the Ca9-22 and HGPEC cells present better targets for bacterial adherence and internalization. The production of the pro-inflammatory chemokine IL-8 is higher in both gingival epithelial cell lines compared to hMSCs and Fusobacterium nucleatum induce a time-dependent cytokine secretion in both cell lines. Porphyromonas gingivalis is less effective in stimulating secretion of IL-8 in the co-cultivation experiments.<h4>Conclusions/significance</h4>HMSCs are suitable for use in anoxic regions of the oral cavity. The interaction with local pathogenic bacteria does not result in massive pro-inflammatory cytokine responses. The test system established in this study allowed further investigation of parameters prior to set up of oral hMSC in vivo studies.
format	article
author	Katja Kriebel Anne Biedermann Bernd Kreikemeyer Hermann Lang
author_facet	Katja Kriebel Anne Biedermann Bernd Kreikemeyer Hermann Lang
author_sort	Katja Kriebel
title	Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.
title_short	Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.
title_full	Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.
title_fullStr	Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.
title_full_unstemmed	Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.
title_sort	anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/2f45b5892d7442c18a970b124a0b94cb
work_keys_str_mv	AT katjakriebel anaerobiccocultureofmesenchymalstemcellsandanaerobicpathogensanewinvitromodelsystem AT annebiedermann anaerobiccocultureofmesenchymalstemcellsandanaerobicpathogensanewinvitromodelsystem AT berndkreikemeyer anaerobiccocultureofmesenchymalstemcellsandanaerobicpathogensanewinvitromodelsystem AT hermannlang anaerobiccocultureofmesenchymalstemcellsandanaerobicpathogensanewinvitromodelsystem
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Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.

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