Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure
Alternative oxidase (AOX) is a key enzyme of the alternative respiration, known to be involved in plant development and in response to various stresses. To verify the role of <i>DcAOX1</i> and <i>DcAOX2a</i> genes in carrot tap root growth and in response to cold stress, thei...
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| Main Authors: | , , , |
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| Format: | article |
| Language: | EN |
| Published: |
MDPI AG
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/2f4d9ccefae346448e1a588d358b4a72 |
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| Summary: | Alternative oxidase (AOX) is a key enzyme of the alternative respiration, known to be involved in plant development and in response to various stresses. To verify the role of <i>DcAOX1</i> and <i>DcAOX2a</i> genes in carrot tap root growth and in response to cold stress, their expression was analyzed in two experiments: during root growth for 13 weeks and in response to a cold challenge trial of 7 days, in both cases using different carrot cultivars. Carrot root growth is initially characterized by an increase in length, followed by a strong increase in weight. <i>DcAOX2a</i> presented the highest expression levels during the initial stages of root growth for all cultivars, but <i>DcAOX1</i> showed no particular trend in expression. Cold stress had a negative impact on root growth, and generally up-regulated <i>DcAOX2a</i> with no consistent effect on <i>DcAOX1</i>. The identification of cis-acting regulatory elements (CAREs) located at the promoters of both genes showed putative sequences involved in cold stress responsiveness, as well as growth. However, <i>DcAOX2a</i> promoter presented more CAREs related to hormonal pathways, including abscisic acid and gibberellins synthesis, than <i>DcAOX1</i>. These results point to a dual role of <i>DcAOX2a</i> on carrot tap root secondary growth and cold stress response. |
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