Carrot AOX2a Transcript Profile Responds to Growth and Chilling Exposure

Alternative oxidase (AOX) is a key enzyme of the alternative respiration, known to be involved in plant development and in response to various stresses. To verify the role of DcAOX1 and DcAOX2a genes in carrot tap root growth and in response to cold stress, thei...

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Autores principales:	Maria Doroteia Campos, Catarina Campos, Amaia Nogales, Hélia Cardoso
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Daucus carota</i> alternative oxidase growth chilling stress <i>cis</i>-elements Botany QK1-989
Acceso en línea:	https://doaj.org/article/2f4d9ccefae346448e1a588d358b4a72
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spelling	oai:doaj.org-article:2f4d9ccefae346448e1a588d358b4a722021-11-25T18:45:54ZCarrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure10.3390/plants101123692223-7747https://doaj.org/article/2f4d9ccefae346448e1a588d358b4a722021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2369https://doaj.org/toc/2223-7747Alternative oxidase (AOX) is a key enzyme of the alternative respiration, known to be involved in plant development and in response to various stresses. To verify the role of <i>DcAOX1</i> and <i>DcAOX2a</i> genes in carrot tap root growth and in response to cold stress, their expression was analyzed in two experiments: during root growth for 13 weeks and in response to a cold challenge trial of 7 days, in both cases using different carrot cultivars. Carrot root growth is initially characterized by an increase in length, followed by a strong increase in weight. <i>DcAOX2a</i> presented the highest expression levels during the initial stages of root growth for all cultivars, but <i>DcAOX1</i> showed no particular trend in expression. Cold stress had a negative impact on root growth, and generally up-regulated <i>DcAOX2a</i> with no consistent effect on <i>DcAOX1</i>. The identification of cis-acting regulatory elements (CAREs) located at the promoters of both genes showed putative sequences involved in cold stress responsiveness, as well as growth. However, <i>DcAOX2a</i> promoter presented more CAREs related to hormonal pathways, including abscisic acid and gibberellins synthesis, than <i>DcAOX1</i>. These results point to a dual role of <i>DcAOX2a</i> on carrot tap root secondary growth and cold stress response.Maria Doroteia CamposCatarina CamposAmaia NogalesHélia CardosoMDPI AGarticle<i>Daucus carota</i>alternative oxidasegrowthchilling stress<i>cis</i>-elementsBotanyQK1-989ENPlants, Vol 10, Iss 2369, p 2369 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Daucus carota</i> alternative oxidase growth chilling stress <i>cis</i>-elements Botany QK1-989
spellingShingle	<i>Daucus carota</i> alternative oxidase growth chilling stress <i>cis</i>-elements Botany QK1-989 Maria Doroteia Campos Catarina Campos Amaia Nogales Hélia Cardoso Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure
description	Alternative oxidase (AOX) is a key enzyme of the alternative respiration, known to be involved in plant development and in response to various stresses. To verify the role of <i>DcAOX1</i> and <i>DcAOX2a</i> genes in carrot tap root growth and in response to cold stress, their expression was analyzed in two experiments: during root growth for 13 weeks and in response to a cold challenge trial of 7 days, in both cases using different carrot cultivars. Carrot root growth is initially characterized by an increase in length, followed by a strong increase in weight. <i>DcAOX2a</i> presented the highest expression levels during the initial stages of root growth for all cultivars, but <i>DcAOX1</i> showed no particular trend in expression. Cold stress had a negative impact on root growth, and generally up-regulated <i>DcAOX2a</i> with no consistent effect on <i>DcAOX1</i>. The identification of cis-acting regulatory elements (CAREs) located at the promoters of both genes showed putative sequences involved in cold stress responsiveness, as well as growth. However, <i>DcAOX2a</i> promoter presented more CAREs related to hormonal pathways, including abscisic acid and gibberellins synthesis, than <i>DcAOX1</i>. These results point to a dual role of <i>DcAOX2a</i> on carrot tap root secondary growth and cold stress response.
format	article
author	Maria Doroteia Campos Catarina Campos Amaia Nogales Hélia Cardoso
author_facet	Maria Doroteia Campos Catarina Campos Amaia Nogales Hélia Cardoso
author_sort	Maria Doroteia Campos
title	Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure
title_short	Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure
title_full	Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure
title_fullStr	Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure
title_full_unstemmed	Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure
title_sort	carrot <i>aox2a</i> transcript profile responds to growth and chilling exposure
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2f4d9ccefae346448e1a588d358b4a72
work_keys_str_mv	AT mariadoroteiacampos carrotiaox2aitranscriptprofilerespondstogrowthandchillingexposure AT catarinacampos carrotiaox2aitranscriptprofilerespondstogrowthandchillingexposure AT amaianogales carrotiaox2aitranscriptprofilerespondstogrowthandchillingexposure AT heliacardoso carrotiaox2aitranscriptprofilerespondstogrowthandchillingexposure
_version_	1718410702089093120

Carrot <i>AOX2a</i> Transcript Profile Responds to Growth and Chilling Exposure

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