Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors

Abstract The aim of this research is twofold: 1) to shed light on zika’s binding and entry mechanism while 2) demonstrating the effectiveness of our magnetic relaxation platform to achieve this goal. Magnetic relaxation-sensitive nanoparticles (MRNPs) are used in a novel fashion to analyze binding i...

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Autores principales:	Tyler Shelby, Tuhina Banerjee, Irene Zegar, Santimukul Santra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f50a8688b7244638129e5ef7c50007d
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spelling	oai:doaj.org-article:2f50a8688b7244638129e5ef7c50007d2021-12-02T15:05:04ZHighly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors10.1038/s41598-017-07620-y2045-2322https://doaj.org/article/2f50a8688b7244638129e5ef7c50007d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07620-yhttps://doaj.org/toc/2045-2322Abstract The aim of this research is twofold: 1) to shed light on zika’s binding and entry mechanism while 2) demonstrating the effectiveness of our magnetic relaxation platform to achieve this goal. Magnetic relaxation-sensitive nanoparticles (MRNPs) are used in a novel fashion to analyze binding interactions between the zika envelope protein (ZENV) and proposed host cell receptors: AXL, HSP70, and TIM-1. Computational analysis is also utilized to examine these binding interactions for the first time. In addition, the role of crizotinib as a potential binding inhibitor is demonstrated and the possibility of ligand-independent phosphatidylserine-mediated binding is explored. Our findings suggest that while the extracellular domain of AXL has the highest affinity for ZENV; HSP70, TIM-1, and phosphatidylserine might also play active roles in zika tropism, which offers a potential explanation for the variety of zika-associated symptoms. This is, to our knowledge, the first time that MRNPs have been used to examine and quantify host-zika interactions. Our magnetic relaxation platform allows for timely and sensitive analysis of these intricate binding relationships, and it is easily customizable for further examination of additional host-pathogen interactions.Tyler ShelbyTuhina BanerjeeIrene ZegarSantimukul SantraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tyler Shelby Tuhina Banerjee Irene Zegar Santimukul Santra Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors
description	Abstract The aim of this research is twofold: 1) to shed light on zika’s binding and entry mechanism while 2) demonstrating the effectiveness of our magnetic relaxation platform to achieve this goal. Magnetic relaxation-sensitive nanoparticles (MRNPs) are used in a novel fashion to analyze binding interactions between the zika envelope protein (ZENV) and proposed host cell receptors: AXL, HSP70, and TIM-1. Computational analysis is also utilized to examine these binding interactions for the first time. In addition, the role of crizotinib as a potential binding inhibitor is demonstrated and the possibility of ligand-independent phosphatidylserine-mediated binding is explored. Our findings suggest that while the extracellular domain of AXL has the highest affinity for ZENV; HSP70, TIM-1, and phosphatidylserine might also play active roles in zika tropism, which offers a potential explanation for the variety of zika-associated symptoms. This is, to our knowledge, the first time that MRNPs have been used to examine and quantify host-zika interactions. Our magnetic relaxation platform allows for timely and sensitive analysis of these intricate binding relationships, and it is easily customizable for further examination of additional host-pathogen interactions.
format	article
author	Tyler Shelby Tuhina Banerjee Irene Zegar Santimukul Santra
author_facet	Tyler Shelby Tuhina Banerjee Irene Zegar Santimukul Santra
author_sort	Tyler Shelby
title	Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors
title_short	Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors
title_full	Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors
title_fullStr	Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors
title_full_unstemmed	Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors
title_sort	highly sensitive, engineered magnetic nanosensors to investigate the ambiguous activity of zika virus and binding receptors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2f50a8688b7244638129e5ef7c50007d
work_keys_str_mv	AT tylershelby highlysensitiveengineeredmagneticnanosensorstoinvestigatetheambiguousactivityofzikavirusandbindingreceptors AT tuhinabanerjee highlysensitiveengineeredmagneticnanosensorstoinvestigatetheambiguousactivityofzikavirusandbindingreceptors AT irenezegar highlysensitiveengineeredmagneticnanosensorstoinvestigatetheambiguousactivityofzikavirusandbindingreceptors AT santimukulsantra highlysensitiveengineeredmagneticnanosensorstoinvestigatetheambiguousactivityofzikavirusandbindingreceptors
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Highly Sensitive, Engineered Magnetic Nanosensors to Investigate the Ambiguous Activity of Zika Virus and Binding Receptors

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