Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity

Abstract Introduction Chronic alcohol consumption (CAC) can induce several deleterious effects on the body, including the promotion of osteoporosis; however, the immunological mechanism underlying alcohol‐induced osteoporosis is still unclear. Methods We administered alcohol to mice for 4 weeks as t...

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Autores principales:	Munehiro Naruo, Yasuyuki Negishi, Takahisa Okuda, Midori Katsuyama, Ken Okazaki, Rimpei Morita
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	alcohol‐induced osteoporosis dendritic cells macrophages NKT‐like cells osteoclast Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/2f546ec108a74e0a9eeeb219efd7d8fe
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spelling	oai:doaj.org-article:2f546ec108a74e0a9eeeb219efd7d8fe2021-11-12T19:57:14ZAlcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity2050-452710.1002/iid3.485https://doaj.org/article/2f546ec108a74e0a9eeeb219efd7d8fe2021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.485https://doaj.org/toc/2050-4527Abstract Introduction Chronic alcohol consumption (CAC) can induce several deleterious effects on the body, including the promotion of osteoporosis; however, the immunological mechanism underlying alcohol‐induced osteoporosis is still unclear. Methods We administered alcohol to mice for 4 weeks as the experimental CAC model and analyzed the bone and immune cells that are located in the vicinity of a bone. Results IL‐4 is known to be a suppressive factor for osteoclastogenesis, and we found that natural killer T (NKT)‐like cells, which showed NK1.1‐positive, CD3‐positive, and α‐galactosylceramide‐loaded CD1d tetramer‐negative, produced IL‐4 more effectively than CD4+ T and natural killer (NK) cells. The alcohol consumption facilitated a significant decrease of bone mineral density with the upregulation of nuclear factor of activated T cells 1 and receptor activator of NF‐κB ligand expression. Meanwhile, we confirmed that alcohol consumption suppressed the activity of antigen‐presenting cells (APCs) and NKT‐like cells, leading to decreased IL‐4 secretion. Moreover, these harmful effects of alcohol consumption were reduced by simultaneous treatment with a glycolipid antigen OCH. Conclusions Our results indicate that the inactivation of innate immune cells, APCs, and NKT‐like cells are likely to be crucial for alcohol‐induced osteoporosis and provide a new therapeutic approach for preventing osteoporosis.Munehiro NaruoYasuyuki NegishiTakahisa OkudaMidori KatsuyamaKen OkazakiRimpei MoritaWileyarticlealcohol‐induced osteoporosisdendritic cellsmacrophagesNKT‐like cellsosteoclastImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1370-1382 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alcohol‐induced osteoporosis dendritic cells macrophages NKT‐like cells osteoclast Immunologic diseases. Allergy RC581-607
spellingShingle	alcohol‐induced osteoporosis dendritic cells macrophages NKT‐like cells osteoclast Immunologic diseases. Allergy RC581-607 Munehiro Naruo Yasuyuki Negishi Takahisa Okuda Midori Katsuyama Ken Okazaki Rimpei Morita Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity
description	Abstract Introduction Chronic alcohol consumption (CAC) can induce several deleterious effects on the body, including the promotion of osteoporosis; however, the immunological mechanism underlying alcohol‐induced osteoporosis is still unclear. Methods We administered alcohol to mice for 4 weeks as the experimental CAC model and analyzed the bone and immune cells that are located in the vicinity of a bone. Results IL‐4 is known to be a suppressive factor for osteoclastogenesis, and we found that natural killer T (NKT)‐like cells, which showed NK1.1‐positive, CD3‐positive, and α‐galactosylceramide‐loaded CD1d tetramer‐negative, produced IL‐4 more effectively than CD4+ T and natural killer (NK) cells. The alcohol consumption facilitated a significant decrease of bone mineral density with the upregulation of nuclear factor of activated T cells 1 and receptor activator of NF‐κB ligand expression. Meanwhile, we confirmed that alcohol consumption suppressed the activity of antigen‐presenting cells (APCs) and NKT‐like cells, leading to decreased IL‐4 secretion. Moreover, these harmful effects of alcohol consumption were reduced by simultaneous treatment with a glycolipid antigen OCH. Conclusions Our results indicate that the inactivation of innate immune cells, APCs, and NKT‐like cells are likely to be crucial for alcohol‐induced osteoporosis and provide a new therapeutic approach for preventing osteoporosis.
format	article
author	Munehiro Naruo Yasuyuki Negishi Takahisa Okuda Midori Katsuyama Ken Okazaki Rimpei Morita
author_facet	Munehiro Naruo Yasuyuki Negishi Takahisa Okuda Midori Katsuyama Ken Okazaki Rimpei Morita
author_sort	Munehiro Naruo
title	Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity
title_short	Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity
title_full	Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity
title_fullStr	Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity
title_full_unstemmed	Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity
title_sort	alcohol consumption induces murine osteoporosis by downregulation of natural killer t‐like cell activity
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/2f546ec108a74e0a9eeeb219efd7d8fe
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_version_	1718430306052079616

Alcohol consumption induces murine osteoporosis by downregulation of natural killer T‐like cell activity

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