An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells

Infection with the avian leukosis virus subgroup J (ALV-J) impairs host genes and facilitates the establishment of chronic infection and the viral life cycle. However, the involvement of long noncoding RNAs (lncRNAs) in ALV-J infection remains largely unknown. In this study, we identified a novel ch...

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Autores principales:	Shihao Chen, Ruihan Zhao, Ting Wu, Dedong Wang, Biao Wang, Shiyu Pan, Xuming Hu, Zhiming Pan, Hengmi Cui
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	ALV-J lncRNA lnc-LTR5B BiP interaction Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2f5c0643aa4d44e9b575d323c6bf3037
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spelling	oai:doaj.org-article:2f5c0643aa4d44e9b575d323c6bf30372021-12-01T13:46:45ZAn Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells1664-302X10.3389/fmicb.2021.788317https://doaj.org/article/2f5c0643aa4d44e9b575d323c6bf30372021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.788317/fullhttps://doaj.org/toc/1664-302XInfection with the avian leukosis virus subgroup J (ALV-J) impairs host genes and facilitates the establishment of chronic infection and the viral life cycle. However, the involvement of long noncoding RNAs (lncRNAs) in ALV-J infection remains largely unknown. In this study, we identified a novel chicken lncRNA derived from LTR5B of the ERV-L family (namely lnc-LTR5B), which is significantly downregulated in ALV-J infected cells. lnc-LTR5B was localized in the cytoplasm and was relatively high expressed in the chicken lung and liver. Notably, the replication of ALV-J was inhibited by the overexpression of lnc-LTR5B but enhanced when lnc-LTR5B expression was knocked down. We further confirmed that lnc-LTR5B could bind to the binding immunoglobulin protein (BiP), a master regulator of endoplasmic reticulum (ER) function. Mechanistically, lnc-LTR5B serves as a competing endogenous RNA for BiP, restricting its physical availability. Upon ALV-J infection, the reduction of lnc-LTR5B released BiP, which facilitated its translocation to the cell surface. This is crucial for ALV-J entry as well as pro-survival signaling. In conclusion, we identified an endogenous retroviral LTR-activated lnc-LTR5B that is involved in regulating the cell surface translocation of BiP, and such regulatory machinery can be exploited by ALV-J to complete its life cycle and propagate.Shihao ChenShihao ChenShihao ChenRuihan ZhaoRuihan ZhaoTing WuTing WuDedong WangDedong WangBiao WangBiao WangShiyu PanShiyu PanXuming HuXuming HuZhiming PanHengmi CuiHengmi CuiHengmi CuiFrontiers Media S.A.articleALV-JlncRNAlnc-LTR5BBiPinteractionMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ALV-J lncRNA lnc-LTR5B BiP interaction Microbiology QR1-502
spellingShingle	ALV-J lncRNA lnc-LTR5B BiP interaction Microbiology QR1-502 Shihao Chen Shihao Chen Shihao Chen Ruihan Zhao Ruihan Zhao Ting Wu Ting Wu Dedong Wang Dedong Wang Biao Wang Biao Wang Shiyu Pan Shiyu Pan Xuming Hu Xuming Hu Zhiming Pan Hengmi Cui Hengmi Cui Hengmi Cui An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
description	Infection with the avian leukosis virus subgroup J (ALV-J) impairs host genes and facilitates the establishment of chronic infection and the viral life cycle. However, the involvement of long noncoding RNAs (lncRNAs) in ALV-J infection remains largely unknown. In this study, we identified a novel chicken lncRNA derived from LTR5B of the ERV-L family (namely lnc-LTR5B), which is significantly downregulated in ALV-J infected cells. lnc-LTR5B was localized in the cytoplasm and was relatively high expressed in the chicken lung and liver. Notably, the replication of ALV-J was inhibited by the overexpression of lnc-LTR5B but enhanced when lnc-LTR5B expression was knocked down. We further confirmed that lnc-LTR5B could bind to the binding immunoglobulin protein (BiP), a master regulator of endoplasmic reticulum (ER) function. Mechanistically, lnc-LTR5B serves as a competing endogenous RNA for BiP, restricting its physical availability. Upon ALV-J infection, the reduction of lnc-LTR5B released BiP, which facilitated its translocation to the cell surface. This is crucial for ALV-J entry as well as pro-survival signaling. In conclusion, we identified an endogenous retroviral LTR-activated lnc-LTR5B that is involved in regulating the cell surface translocation of BiP, and such regulatory machinery can be exploited by ALV-J to complete its life cycle and propagate.
format	article
author	Shihao Chen Shihao Chen Shihao Chen Ruihan Zhao Ruihan Zhao Ting Wu Ting Wu Dedong Wang Dedong Wang Biao Wang Biao Wang Shiyu Pan Shiyu Pan Xuming Hu Xuming Hu Zhiming Pan Hengmi Cui Hengmi Cui Hengmi Cui
author_facet	Shihao Chen Shihao Chen Shihao Chen Ruihan Zhao Ruihan Zhao Ting Wu Ting Wu Dedong Wang Dedong Wang Biao Wang Biao Wang Shiyu Pan Shiyu Pan Xuming Hu Xuming Hu Zhiming Pan Hengmi Cui Hengmi Cui Hengmi Cui
author_sort	Shihao Chen
title	An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
title_short	An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
title_full	An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
title_fullStr	An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
title_full_unstemmed	An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
title_sort	endogenous retroviral ltr-derived long noncoding rna lnc-ltr5b interacts with bip to modulate alv-j replication in chicken cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2f5c0643aa4d44e9b575d323c6bf3037
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An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells

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