<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus

ABSTRACT Legionella pneumophila is the causative agent of a pneumonia termed Legionnaires’ disease. The facultative intracellular bacterium employs the Icm/Dot type IV secretion system (T4SS) and a plethora of translocated “effector” proteins to interfere with host vesicle trafficking pathways and e...

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Autores principales:	Stephen Weber, Bernhard Steiner, Amanda Welin, Hubert Hilbi
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Amoeba Dictyostelium Golgi apparatus Legionella effector protein host-pathogen interaction Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2f73646547b74bbc99afaeccf7064cc4
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spelling	oai:doaj.org-article:2f73646547b74bbc99afaeccf7064cc42021-11-15T15:52:19Z<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus10.1128/mBio.02420-182150-7511https://doaj.org/article/2f73646547b74bbc99afaeccf7064cc42018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02420-18https://doaj.org/toc/2150-7511ABSTRACT Legionella pneumophila is the causative agent of a pneumonia termed Legionnaires’ disease. The facultative intracellular bacterium employs the Icm/Dot type IV secretion system (T4SS) and a plethora of translocated “effector” proteins to interfere with host vesicle trafficking pathways and establish a replicative niche, the Legionella-containing vacuole (LCV). Internalization of the pathogen and the events immediately ensuing are accompanied by host cell-mediated phosphoinositide (PI) lipid changes and the Icm/Dot-controlled conversion of the LCV from a PtdIns(3)P-positive vacuole into a PtdIns(4)P-positive replication-permissive compartment, which tightly associates with the endoplasmic reticulum. The source and formation of PtdIns(4)P are ill-defined. Using dually labeled Dictyostelium discoideum amoebae and real-time high-resolution confocal laser scanning microscopy (CLSM), we show here that nascent LCVs continuously capture and accumulate PtdIns(4)P-positive vesicles from the host cell. Trafficking of these PtdIns(4)P-positive vesicles to LCVs occurs independently of the Icm/Dot system, but their sustained association requires a functional T4SS. During the infection, PtdIns(3)P-positive membranes become compacted and segregated from the LCV, and PtdIns(3)P-positive vesicles traffic to the LCV but do not fuse. Moreover, using eukaryotic and prokaryotic PtdIns(4)P probes (2×PHFAPP-green fluorescent protein [2×PHFAPP-GFP] and P4CSidC-GFP, respectively) along with Arf1-GFP, we show that PtdIns(4)P-rich membranes of the trans-Golgi network associate with the LCV. Intriguingly, the interaction dynamics of 2×PHFAPP-GFP and P4CSidC-GFP are spatially separable and reveal the specific PtdIns(4)P pool from which the LCV PI originates. These findings provide high-resolution real-time insights into how L. pneumophila exploits the cellular dynamics of membrane-bound PtdIns(4)P for LCV formation. IMPORTANCE The environmental bacterium Legionella pneumophila causes a life-threatening pneumonia termed Legionnaires’ disease. The bacteria grow intracellularly in free-living amoebae as well as in respiratory tract macrophages. To this end, L. pneumophila forms a distinct membrane-bound compartment called the Legionella-containing vacuole (LCV). Phosphoinositide (PI) lipids are crucial regulators of the identity and dynamics of host cell organelles. The PI lipid PtdIns(4)P is a hallmark of the host cell secretory pathway, and decoration of LCVs with this PI is required for pathogen vacuole maturation. The source, dynamics, and mode of accumulation of PtdIns(4)P on LCVs are largely unknown. Using Dictyostelium amoebae producing different fluorescent probes as host cells, we show here that LCVs rapidly acquire PtdIns(4)P through the continuous interaction with PtdIns(4)P-positive host vesicles derived from the Golgi apparatus. Thus, the PI lipid pattern of the secretory pathway contributes to the formation of the replication-permissive pathogen compartment.Stephen WeberBernhard SteinerAmanda WelinHubert HilbiAmerican Society for MicrobiologyarticleAmoebaDictyosteliumGolgi apparatusLegionellaeffector proteinhost-pathogen interactionMicrobiologyQR1-502ENmBio, Vol 9, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Amoeba Dictyostelium Golgi apparatus Legionella effector protein host-pathogen interaction Microbiology QR1-502
spellingShingle	Amoeba Dictyostelium Golgi apparatus Legionella effector protein host-pathogen interaction Microbiology QR1-502 Stephen Weber Bernhard Steiner Amanda Welin Hubert Hilbi <italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus
description	ABSTRACT Legionella pneumophila is the causative agent of a pneumonia termed Legionnaires’ disease. The facultative intracellular bacterium employs the Icm/Dot type IV secretion system (T4SS) and a plethora of translocated “effector” proteins to interfere with host vesicle trafficking pathways and establish a replicative niche, the Legionella-containing vacuole (LCV). Internalization of the pathogen and the events immediately ensuing are accompanied by host cell-mediated phosphoinositide (PI) lipid changes and the Icm/Dot-controlled conversion of the LCV from a PtdIns(3)P-positive vacuole into a PtdIns(4)P-positive replication-permissive compartment, which tightly associates with the endoplasmic reticulum. The source and formation of PtdIns(4)P are ill-defined. Using dually labeled Dictyostelium discoideum amoebae and real-time high-resolution confocal laser scanning microscopy (CLSM), we show here that nascent LCVs continuously capture and accumulate PtdIns(4)P-positive vesicles from the host cell. Trafficking of these PtdIns(4)P-positive vesicles to LCVs occurs independently of the Icm/Dot system, but their sustained association requires a functional T4SS. During the infection, PtdIns(3)P-positive membranes become compacted and segregated from the LCV, and PtdIns(3)P-positive vesicles traffic to the LCV but do not fuse. Moreover, using eukaryotic and prokaryotic PtdIns(4)P probes (2×PHFAPP-green fluorescent protein [2×PHFAPP-GFP] and P4CSidC-GFP, respectively) along with Arf1-GFP, we show that PtdIns(4)P-rich membranes of the trans-Golgi network associate with the LCV. Intriguingly, the interaction dynamics of 2×PHFAPP-GFP and P4CSidC-GFP are spatially separable and reveal the specific PtdIns(4)P pool from which the LCV PI originates. These findings provide high-resolution real-time insights into how L. pneumophila exploits the cellular dynamics of membrane-bound PtdIns(4)P for LCV formation. IMPORTANCE The environmental bacterium Legionella pneumophila causes a life-threatening pneumonia termed Legionnaires’ disease. The bacteria grow intracellularly in free-living amoebae as well as in respiratory tract macrophages. To this end, L. pneumophila forms a distinct membrane-bound compartment called the Legionella-containing vacuole (LCV). Phosphoinositide (PI) lipids are crucial regulators of the identity and dynamics of host cell organelles. The PI lipid PtdIns(4)P is a hallmark of the host cell secretory pathway, and decoration of LCVs with this PI is required for pathogen vacuole maturation. The source, dynamics, and mode of accumulation of PtdIns(4)P on LCVs are largely unknown. Using Dictyostelium amoebae producing different fluorescent probes as host cells, we show here that LCVs rapidly acquire PtdIns(4)P through the continuous interaction with PtdIns(4)P-positive host vesicles derived from the Golgi apparatus. Thus, the PI lipid pattern of the secretory pathway contributes to the formation of the replication-permissive pathogen compartment.
format	article
author	Stephen Weber Bernhard Steiner Amanda Welin Hubert Hilbi
author_facet	Stephen Weber Bernhard Steiner Amanda Welin Hubert Hilbi
author_sort	Stephen Weber
title	<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus
title_short	<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus
title_full	<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus
title_fullStr	<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus
title_full_unstemmed	<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus
title_sort	<italic toggle="yes">legionella</italic>-containing vacuoles capture ptdins(4)<italic toggle="yes">p</italic>-rich vesicles derived from the golgi apparatus
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/2f73646547b74bbc99afaeccf7064cc4
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<italic toggle="yes">Legionella</italic>-Containing Vacuoles Capture PtdIns(4)<italic toggle="yes">P</italic>-Rich Vesicles Derived from the Golgi Apparatus

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