Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp

Understanding how Mesenchymal Stem Cells (MSCs) form blood vessels is critical for creating mechanism-based approaches for the therapeutic use of these cells. In addition, understanding the determinants and factors involved in lineage hierarchy is fundamental to creating accurate and reliable techni...

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Autores principales:	Andrea Mantesso, Zhaocheng Zhang, Kristy A. Warner, Alexandra E. Herzog, Ajai J. Pulianmackal, Jacques E. Nör
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	vasculogenesis angiogenesis tissue regeneration endothelial cells cell fate stemness Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2f73ad61635447c5924ac54b3b159172
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spelling	oai:doaj.org-article:2f73ad61635447c5924ac54b3b1591722021-11-25T17:07:15ZPulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp10.3390/cells101128042073-4409https://doaj.org/article/2f73ad61635447c5924ac54b3b1591722021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2804https://doaj.org/toc/2073-4409Understanding how Mesenchymal Stem Cells (MSCs) form blood vessels is critical for creating mechanism-based approaches for the therapeutic use of these cells. In addition, understanding the determinants and factors involved in lineage hierarchy is fundamental to creating accurate and reliable techniques for the study of stem cells in tissue engineering and repair. Dental Pulp Stem Cells (DPSC) from permanent teeth and Stem cells from Human Exfoliated Deciduous teeth (SHED) are particularly interesting sources for tissue engineering as they are easily accessible and expandable. Previously, we have shown that DPSCs and SHEDs can differentiate into endothelial cells and form functional blood vessels through vasculogenesis. Here, we described how we created the “pulpbow” (pulp + rainbow), a multicolor tag experimental model that is stable, permanent, unique to each cell and passed through generations. We used the pulpbow to understand how dental pulp stem cells contributed to blood vessel formation in 3D models in in vitro and ex vivo live cell tracking, and in vivo transplantation assays. Simultaneous tracking of cells during sprout formation revealed that no single multicolor-tagged cell was more prone to vasculogenesis. During this process, there was intense cell motility with minimal proliferation in early time points. In later stages, when the availability of undifferentiated cells around the forming sprout decreased, there was local clonal proliferation mediated by proximity. These results unveiled that the vasculogenesis process mediated by dental pulp stem cells is dynamic and proximity to the sprouting area is critical for cell fate decisions.Andrea MantessoZhaocheng ZhangKristy A. WarnerAlexandra E. HerzogAjai J. PulianmackalJacques E. NörMDPI AGarticlevasculogenesisangiogenesistissue regenerationendothelial cellscell fatestemnessBiology (General)QH301-705.5ENCells, Vol 10, Iss 2804, p 2804 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vasculogenesis angiogenesis tissue regeneration endothelial cells cell fate stemness Biology (General) QH301-705.5
spellingShingle	vasculogenesis angiogenesis tissue regeneration endothelial cells cell fate stemness Biology (General) QH301-705.5 Andrea Mantesso Zhaocheng Zhang Kristy A. Warner Alexandra E. Herzog Ajai J. Pulianmackal Jacques E. Nör Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp
description	Understanding how Mesenchymal Stem Cells (MSCs) form blood vessels is critical for creating mechanism-based approaches for the therapeutic use of these cells. In addition, understanding the determinants and factors involved in lineage hierarchy is fundamental to creating accurate and reliable techniques for the study of stem cells in tissue engineering and repair. Dental Pulp Stem Cells (DPSC) from permanent teeth and Stem cells from Human Exfoliated Deciduous teeth (SHED) are particularly interesting sources for tissue engineering as they are easily accessible and expandable. Previously, we have shown that DPSCs and SHEDs can differentiate into endothelial cells and form functional blood vessels through vasculogenesis. Here, we described how we created the “pulpbow” (pulp + rainbow), a multicolor tag experimental model that is stable, permanent, unique to each cell and passed through generations. We used the pulpbow to understand how dental pulp stem cells contributed to blood vessel formation in 3D models in in vitro and ex vivo live cell tracking, and in vivo transplantation assays. Simultaneous tracking of cells during sprout formation revealed that no single multicolor-tagged cell was more prone to vasculogenesis. During this process, there was intense cell motility with minimal proliferation in early time points. In later stages, when the availability of undifferentiated cells around the forming sprout decreased, there was local clonal proliferation mediated by proximity. These results unveiled that the vasculogenesis process mediated by dental pulp stem cells is dynamic and proximity to the sprouting area is critical for cell fate decisions.
format	article
author	Andrea Mantesso Zhaocheng Zhang Kristy A. Warner Alexandra E. Herzog Ajai J. Pulianmackal Jacques E. Nör
author_facet	Andrea Mantesso Zhaocheng Zhang Kristy A. Warner Alexandra E. Herzog Ajai J. Pulianmackal Jacques E. Nör
author_sort	Andrea Mantesso
title	Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp
title_short	Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp
title_full	Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp
title_fullStr	Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp
title_full_unstemmed	Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp
title_sort	pulpbow: a method to study the vasculogenic potential of mesenchymal stem cells from the dental pulp
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2f73ad61635447c5924ac54b3b159172
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Pulpbow: A Method to Study the Vasculogenic Potential of Mesenchymal Stem Cells from the Dental Pulp

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