Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.

Geobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machin...

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Autores principales:	Adam M Feist, Harish Nagarajan, Amelia-Elena Rotaru, Pier-Luc Tremblay, Tian Zhang, Kelly P Nevin, Derek R Lovley, Karsten Zengler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2f7da3511c5a43a1a60fcf00f39f5dc2
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spelling	oai:doaj.org-article:2f7da3511c5a43a1a60fcf00f39f5dc22021-11-18T05:52:56ZConstraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.1553-734X1553-735810.1371/journal.pcbi.1003575https://doaj.org/article/2f7da3511c5a43a1a60fcf00f39f5dc22014-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24762737/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Geobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machinery of Geobacter metallireducens GS-15 to carry out CO2 fixation and direct electron transfer to iron. An updated metabolic reconstruction was generated, growth screens on targeted conditions of interest were performed, and constraint-based analysis was utilized to characterize and evaluate critical pathways and reactions in G. metallireducens. The novel capability of G. metallireducens to grow autotrophically with formate and Fe(III) was predicted and subsequently validated in vivo. Additionally, the energetic cost of transferring electrons to an external electron acceptor was determined through analysis of growth experiments carried out using three different electron acceptors (Fe(III), nitrate, and fumarate) by systematically isolating and examining different parts of the electron transport chain. The updated reconstruction will serve as a knowledgebase for understanding and engineering Geobacter and similar species.Adam M FeistHarish NagarajanAmelia-Elena RotaruPier-Luc TremblayTian ZhangKelly P NevinDerek R LovleyKarsten ZenglerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 10, Iss 4, p e1003575 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Adam M Feist Harish Nagarajan Amelia-Elena Rotaru Pier-Luc Tremblay Tian Zhang Kelly P Nevin Derek R Lovley Karsten Zengler Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.
description	Geobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machinery of Geobacter metallireducens GS-15 to carry out CO2 fixation and direct electron transfer to iron. An updated metabolic reconstruction was generated, growth screens on targeted conditions of interest were performed, and constraint-based analysis was utilized to characterize and evaluate critical pathways and reactions in G. metallireducens. The novel capability of G. metallireducens to grow autotrophically with formate and Fe(III) was predicted and subsequently validated in vivo. Additionally, the energetic cost of transferring electrons to an external electron acceptor was determined through analysis of growth experiments carried out using three different electron acceptors (Fe(III), nitrate, and fumarate) by systematically isolating and examining different parts of the electron transport chain. The updated reconstruction will serve as a knowledgebase for understanding and engineering Geobacter and similar species.
format	article
author	Adam M Feist Harish Nagarajan Amelia-Elena Rotaru Pier-Luc Tremblay Tian Zhang Kelly P Nevin Derek R Lovley Karsten Zengler
author_facet	Adam M Feist Harish Nagarajan Amelia-Elena Rotaru Pier-Luc Tremblay Tian Zhang Kelly P Nevin Derek R Lovley Karsten Zengler
author_sort	Adam M Feist
title	Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.
title_short	Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.
title_full	Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.
title_fullStr	Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.
title_full_unstemmed	Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.
title_sort	constraint-based modeling of carbon fixation and the energetics of electron transfer in geobacter metallireducens.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2f7da3511c5a43a1a60fcf00f39f5dc2
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Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.

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