Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses

ABSTRACT Pollution from nutrients in aquatic habitats has been linked to increases in disease vectors, including mosquitoes and other pestiferous insects. One possibility is that changes in mosquito microbiomes are impacted by nutrient enrichments and that these changes affect various traits, includ...

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Autores principales:	Dagne Duguma, Michael W. Hall, Chelsea T. Smartt, Josh D. Neufeld
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	aquatic chemistry bacteria disease vectors food web life stages microbiome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2f7f40084543442991e80252c26eed9c
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spelling	oai:doaj.org-article:2f7f40084543442991e80252c26eed9c2021-11-15T15:22:03ZEffects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses10.1128/mSphere.00387-162379-5042https://doaj.org/article/2f7f40084543442991e80252c26eed9c2017-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00387-16https://doaj.org/toc/2379-5042ABSTRACT Pollution from nutrients in aquatic habitats has been linked to increases in disease vectors, including mosquitoes and other pestiferous insects. One possibility is that changes in mosquito microbiomes are impacted by nutrient enrichments and that these changes affect various traits, including larval development, susceptibility to larval control agents, and susceptibility of the adult mosquitoes to pathogens. We tested this hypothesis using field mesocosms supplemented with low- and high-organic-nutrient regimens and then sampled microbial communities associated with the naturally colonizing Culex nigripalpus mosquito vector. By high-throughput sequencing of 16S rRNA gene sequences, we found no significant differences in overall microbial communities associated with sampled mosquitoes, despite detecting discernible differences in environmental variables, including pH, dissolved oxygen, and nutrient amendments. Nevertheless, indicator species analysis revealed that members of the Clostridiales were significantly associated with mosquitoes that originated from high-nutrient enrichments. In contrast, members of the Burkholderiales were associated with mosquitoes from the low-nutrient enrichment. High bacterial variability associated with the life stages of the C. nigripalpus was largely unaffected by levels of nutrient enrichments that impacted larval microbial resources, including bacteria, ciliates, and flagellates in the larval environments. IMPORTANCE Mosquito microbiota provide important physiological and ecological attributes to mosquitoes, including an impact on their susceptibility to pathogens, fitness, and sensitivity to mosquito control agents. Culex nigripalpus mosquito populations transmit various pathogens, including the Saint Louis and West Nile viruses, and proliferate in nutrient-rich environments, such as in wastewater treatment wetlands. Our study examined whether increases in nutrients within larval mosquito developmental habitats impact microbial communities associated with C. nigripalpus mosquitoes. We characterized the effects of organic enrichments on microbiomes associated with C. nigripalpus mosquitoes and identified potential bacterial microbiota that will be further investigated for whether they alter mosquito life history traits and for their potential role in the development of microbial-based control strategies.Dagne DugumaMichael W. HallChelsea T. SmarttJosh D. NeufeldAmerican Society for Microbiologyarticleaquatic chemistrybacteriadisease vectorsfood weblife stagesmicrobiomeMicrobiologyQR1-502ENmSphere, Vol 2, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	aquatic chemistry bacteria disease vectors food web life stages microbiome Microbiology QR1-502
spellingShingle	aquatic chemistry bacteria disease vectors food web life stages microbiome Microbiology QR1-502 Dagne Duguma Michael W. Hall Chelsea T. Smartt Josh D. Neufeld Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses
description	ABSTRACT Pollution from nutrients in aquatic habitats has been linked to increases in disease vectors, including mosquitoes and other pestiferous insects. One possibility is that changes in mosquito microbiomes are impacted by nutrient enrichments and that these changes affect various traits, including larval development, susceptibility to larval control agents, and susceptibility of the adult mosquitoes to pathogens. We tested this hypothesis using field mesocosms supplemented with low- and high-organic-nutrient regimens and then sampled microbial communities associated with the naturally colonizing Culex nigripalpus mosquito vector. By high-throughput sequencing of 16S rRNA gene sequences, we found no significant differences in overall microbial communities associated with sampled mosquitoes, despite detecting discernible differences in environmental variables, including pH, dissolved oxygen, and nutrient amendments. Nevertheless, indicator species analysis revealed that members of the Clostridiales were significantly associated with mosquitoes that originated from high-nutrient enrichments. In contrast, members of the Burkholderiales were associated with mosquitoes from the low-nutrient enrichment. High bacterial variability associated with the life stages of the C. nigripalpus was largely unaffected by levels of nutrient enrichments that impacted larval microbial resources, including bacteria, ciliates, and flagellates in the larval environments. IMPORTANCE Mosquito microbiota provide important physiological and ecological attributes to mosquitoes, including an impact on their susceptibility to pathogens, fitness, and sensitivity to mosquito control agents. Culex nigripalpus mosquito populations transmit various pathogens, including the Saint Louis and West Nile viruses, and proliferate in nutrient-rich environments, such as in wastewater treatment wetlands. Our study examined whether increases in nutrients within larval mosquito developmental habitats impact microbial communities associated with C. nigripalpus mosquitoes. We characterized the effects of organic enrichments on microbiomes associated with C. nigripalpus mosquitoes and identified potential bacterial microbiota that will be further investigated for whether they alter mosquito life history traits and for their potential role in the development of microbial-based control strategies.
format	article
author	Dagne Duguma Michael W. Hall Chelsea T. Smartt Josh D. Neufeld
author_facet	Dagne Duguma Michael W. Hall Chelsea T. Smartt Josh D. Neufeld
author_sort	Dagne Duguma
title	Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses
title_short	Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses
title_full	Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses
title_fullStr	Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses
title_full_unstemmed	Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses
title_sort	effects of organic amendments on microbiota associated with the <named-content content-type="genus-species">culex nigripalpus</named-content> mosquito vector of the saint louis encephalitis and west nile viruses
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/2f7f40084543442991e80252c26eed9c
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Effects of Organic Amendments on Microbiota Associated with the <named-content content-type="genus-species">Culex nigripalpus</named-content> Mosquito Vector of the Saint Louis Encephalitis and West Nile Viruses

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