Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo

Zhenguang Liu,1,2 Lin Yu,1,2 Pengfei Gu,1,2 Ruonan Bo,1,2 Shuwen Xu,1,2 Adelijiang Wusiman,1,2 Jiaguo Liu,1,2 Yuanliang Hu,1,2 Deyun Wang1,2 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Repu...

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Autores principales:	Liu Z, Yu L, Gu P, Bo R, Xu S, Wusiman A, Liu J, Hu Y, Wang D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cubosomes;ganoderma lucidum polysaccharide surface-engineering immune response rna-seq Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2f8a1c69149c4fb7ae572ad376591874
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spelling	oai:doaj.org-article:2f8a1c69149c4fb7ae572ad3765918742021-12-02T13:42:12ZSurface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo1178-2013https://doaj.org/article/2f8a1c69149c4fb7ae572ad3765918742020-11-01T00:00:00Zhttps://www.dovepress.com/surface-engineered-cubosomes-serve-as-a-novel-vaccine-adjuvant-to-modu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhenguang Liu,1,2 Lin Yu,1,2 Pengfei Gu,1,2 Ruonan Bo,1,2 Shuwen Xu,1,2 Adelijiang Wusiman,1,2 Jiaguo Liu,1,2 Yuanliang Hu,1,2 Deyun Wang1,2 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China; 2MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of ChinaCorrespondence: Deyun WangInstitute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of ChinaTel +86-25-84395203Fax +86-25-84398669Email dywang@njau.edu.cnObjective: Recent studies have revealed the adjuvant activity of cubosomes and their potential utility as an antigen delivery system. In this study, to further enhance the adjuvant activity of cubosomes, two cationic polymers are modified on the surface of cubosomes.Methods: Here, we exploit the effects of surface chemistry on the adjuvant activity of Ganoderma lucidum polysaccharide cubosomes (GLPC) by placing two kinds of molecules, that is, cetyltrimethylammonium bromide (CTAB) and poly(diallydimethyl ammonium chloride) (PDDAC), on their surface.Results: CTAB- or PDDAC-modified GLPC were found to significantly promote humoral and cellular immune responses, as well as the proliferation of CD3+ CD4+ or CD3+ CD8+T cells through the powerful activation of dendritic cells (DCs). The enhanced immune responses of PDDAC-modified GLPC might be attributed to the maturation of DCs into draining lymph nodes and the activation of spleen and cytokines in serum.Conclusion: PDDAC modification is beneficial for enhancing humoral and cellular immune response, suggesting that PDDAC-GLPC-OVA has the ability to be a potential adjuvant for vaccine.Keywords: cubosomes, Ganoderma lucidum polysaccharide, surface-engineering, immune response, RNA-seqLiu ZYu LGu PBo RXu SWusiman ALiu JHu YWang DDove Medical Pressarticlecubosomes;ganoderma lucidum polysaccharidesurface-engineeringimmune responserna-seqMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 8595-8608 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cubosomes;ganoderma lucidum polysaccharide surface-engineering immune response rna-seq Medicine (General) R5-920
spellingShingle	cubosomes;ganoderma lucidum polysaccharide surface-engineering immune response rna-seq Medicine (General) R5-920 Liu Z Yu L Gu P Bo R Xu S Wusiman A Liu J Hu Y Wang D Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo
description	Zhenguang Liu,1,2 Lin Yu,1,2 Pengfei Gu,1,2 Ruonan Bo,1,2 Shuwen Xu,1,2 Adelijiang Wusiman,1,2 Jiaguo Liu,1,2 Yuanliang Hu,1,2 Deyun Wang1,2 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China; 2MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of ChinaCorrespondence: Deyun WangInstitute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of ChinaTel +86-25-84395203Fax +86-25-84398669Email dywang@njau.edu.cnObjective: Recent studies have revealed the adjuvant activity of cubosomes and their potential utility as an antigen delivery system. In this study, to further enhance the adjuvant activity of cubosomes, two cationic polymers are modified on the surface of cubosomes.Methods: Here, we exploit the effects of surface chemistry on the adjuvant activity of Ganoderma lucidum polysaccharide cubosomes (GLPC) by placing two kinds of molecules, that is, cetyltrimethylammonium bromide (CTAB) and poly(diallydimethyl ammonium chloride) (PDDAC), on their surface.Results: CTAB- or PDDAC-modified GLPC were found to significantly promote humoral and cellular immune responses, as well as the proliferation of CD3+ CD4+ or CD3+ CD8+T cells through the powerful activation of dendritic cells (DCs). The enhanced immune responses of PDDAC-modified GLPC might be attributed to the maturation of DCs into draining lymph nodes and the activation of spleen and cytokines in serum.Conclusion: PDDAC modification is beneficial for enhancing humoral and cellular immune response, suggesting that PDDAC-GLPC-OVA has the ability to be a potential adjuvant for vaccine.Keywords: cubosomes, Ganoderma lucidum polysaccharide, surface-engineering, immune response, RNA-seq
format	article
author	Liu Z Yu L Gu P Bo R Xu S Wusiman A Liu J Hu Y Wang D
author_facet	Liu Z Yu L Gu P Bo R Xu S Wusiman A Liu J Hu Y Wang D
author_sort	Liu Z
title	Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo
title_short	Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo
title_full	Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo
title_fullStr	Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo
title_full_unstemmed	Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo
title_sort	surface-engineered cubosomes serve as a novel vaccine adjuvant to modulate innate immunity and improve adaptive immunity in vivo
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/2f8a1c69149c4fb7ae572ad376591874
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Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo

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