A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1

Candida glabrata is a prominent pathogenic yeast which exhibits a unique ability to survive the harsh environment of host immune cells. In this study, we describe the role of the transcription factor encoded by the gene CAGL0F09229g, here named CgTog1 after its Saccharomyces cerevisiae ortholog, as...

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Autores principales:	Pedro Pais, Susana Vagueiro, Dalila Mil-Homens, Andreia I. Pimenta, Romeu Viana, Michiyo Okamoto, Hiroji Chibana, Arsénio M. Fialho, Miguel C. Teixeira
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	candida glabrata virulence phagocytosis cgtog1 oxidative stress rna-seq based transcriptomics Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/2f9722627f254045840f9e3363b6af92
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spelling	oai:doaj.org-article:2f9722627f254045840f9e3363b6af922021-11-17T14:21:59ZA new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog12150-55942150-560810.1080/21505594.2020.1839231https://doaj.org/article/2f9722627f254045840f9e3363b6af922020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1839231https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Candida glabrata is a prominent pathogenic yeast which exhibits a unique ability to survive the harsh environment of host immune cells. In this study, we describe the role of the transcription factor encoded by the gene CAGL0F09229g, here named CgTog1 after its Saccharomyces cerevisiae ortholog, as a new determinant of C. glabrata virulence. Interestingly, Tog1 is absent in the other clinically relevant Candida species (C. albicans, C. parapsilosis, C. tropicalis, C. auris), being exclusive to C. glabrata. CgTog1 was found to be required for oxidative stress resistance and for the modulation of reactive oxygen species inside C. glabrata cells. Also, CgTog1 was observed to be a nuclear protein, whose activity up-regulates the expression of 147 genes and represses 112 genes in C. glabrata cells exposed to H2O2, as revealed through RNA-seq-based transcriptomics analysis. Given the importance of oxidative stress response in the resistance to host immune cells, the effect of CgTOG1 expression in yeast survival upon phagocytosis by Galleria mellonella hemocytes was evaluated, leading to the identification of CgTog1 as a determinant of yeast survival upon phagocytosis. Interestingly, CgTog1 targets include many whose expression changes in C. glabrata cells after engulfment by macrophages, including those involved in reprogrammed carbon metabolism, glyoxylate cycle and fatty acid degradation. In summary, CgTog1 is a new and specific regulator of virulence in C. glabrata, contributing to oxidative stress resistance and survival upon phagocytosis by host immune cells.Pedro PaisSusana VagueiroDalila Mil-HomensAndreia I. PimentaRomeu VianaMichiyo OkamotoHiroji ChibanaArsénio M. FialhoMiguel C. TeixeiraTaylor & Francis Grouparticlecandida glabratavirulencephagocytosiscgtog1oxidative stressrna-seq based transcriptomicsInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1522-1538 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	candida glabrata virulence phagocytosis cgtog1 oxidative stress rna-seq based transcriptomics Infectious and parasitic diseases RC109-216
spellingShingle	candida glabrata virulence phagocytosis cgtog1 oxidative stress rna-seq based transcriptomics Infectious and parasitic diseases RC109-216 Pedro Pais Susana Vagueiro Dalila Mil-Homens Andreia I. Pimenta Romeu Viana Michiyo Okamoto Hiroji Chibana Arsénio M. Fialho Miguel C. Teixeira A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
description	Candida glabrata is a prominent pathogenic yeast which exhibits a unique ability to survive the harsh environment of host immune cells. In this study, we describe the role of the transcription factor encoded by the gene CAGL0F09229g, here named CgTog1 after its Saccharomyces cerevisiae ortholog, as a new determinant of C. glabrata virulence. Interestingly, Tog1 is absent in the other clinically relevant Candida species (C. albicans, C. parapsilosis, C. tropicalis, C. auris), being exclusive to C. glabrata. CgTog1 was found to be required for oxidative stress resistance and for the modulation of reactive oxygen species inside C. glabrata cells. Also, CgTog1 was observed to be a nuclear protein, whose activity up-regulates the expression of 147 genes and represses 112 genes in C. glabrata cells exposed to H2O2, as revealed through RNA-seq-based transcriptomics analysis. Given the importance of oxidative stress response in the resistance to host immune cells, the effect of CgTOG1 expression in yeast survival upon phagocytosis by Galleria mellonella hemocytes was evaluated, leading to the identification of CgTog1 as a determinant of yeast survival upon phagocytosis. Interestingly, CgTog1 targets include many whose expression changes in C. glabrata cells after engulfment by macrophages, including those involved in reprogrammed carbon metabolism, glyoxylate cycle and fatty acid degradation. In summary, CgTog1 is a new and specific regulator of virulence in C. glabrata, contributing to oxidative stress resistance and survival upon phagocytosis by host immune cells.
format	article
author	Pedro Pais Susana Vagueiro Dalila Mil-Homens Andreia I. Pimenta Romeu Viana Michiyo Okamoto Hiroji Chibana Arsénio M. Fialho Miguel C. Teixeira
author_facet	Pedro Pais Susana Vagueiro Dalila Mil-Homens Andreia I. Pimenta Romeu Viana Michiyo Okamoto Hiroji Chibana Arsénio M. Fialho Miguel C. Teixeira
author_sort	Pedro Pais
title	A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_short	A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_full	A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_fullStr	A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_full_unstemmed	A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1
title_sort	new regulator in the crossroads of oxidative stress resistance and virulence in candida glabrata: the transcription factor cgtog1
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/2f9722627f254045840f9e3363b6af92
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A new regulator in the crossroads of oxidative stress resistance and virulence in Candida glabrata: The transcription factor CgTog1

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