Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.

The availability of the complete genome sequence of several Salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. This is the case of Salmonella enterica serovar Enteritidis (S. Enteritidis), a bacterium th...

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Autores principales:	Tania S Quiroz, Pamela A Nieto, Hugo E Tobar, Francisco J Salazar-Echegarai, Rodrigo J Lizana, Carolina P Quezada, Carlos A Santiviago, Daniela V Araya, Claudia A Riedel, Alexis M Kalergis, Susan M Bueno
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f98ca31db9043c0ae02f2ce0f0471c7
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spelling	oai:doaj.org-article:2f98ca31db9043c0ae02f2ce0f0471c72021-11-18T07:36:10ZExcision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.1932-620310.1371/journal.pone.0026031https://doaj.org/article/2f98ca31db9043c0ae02f2ce0f0471c72011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039432/?tool=EBIhttps://doaj.org/toc/1932-6203The availability of the complete genome sequence of several Salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. This is the case of Salmonella enterica serovar Enteritidis (S. Enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. The whole genome sequence analysis for S. Enteritidis unveiled the presence of several genetic regions that are absent in other Salmonella serovars. These regions have been denominated "regions of difference" (ROD). In this study we show that ROD21, one of such regions, behaves as an unstable pathogenicity island. We observed that ROD21 undergoes spontaneous excision by two independent recombination events, either under laboratory growth conditions or during infection of murine cells. Importantly, we also found that one type of excision occurred at higher rates when S. Enteritidis was residing inside murine phagocytic cells. These data suggest that ROD21 is an unstable pathogenicity island, whose frequency of excision depends on the environmental conditions found inside phagocytic cells.Tania S QuirozPamela A NietoHugo E TobarFrancisco J Salazar-EchegaraiRodrigo J LizanaCarolina P QuezadaCarlos A SantiviagoDaniela V ArayaClaudia A RiedelAlexis M KalergisSusan M BuenoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26031 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tania S Quiroz Pamela A Nieto Hugo E Tobar Francisco J Salazar-Echegarai Rodrigo J Lizana Carolina P Quezada Carlos A Santiviago Daniela V Araya Claudia A Riedel Alexis M Kalergis Susan M Bueno Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.
description	The availability of the complete genome sequence of several Salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. This is the case of Salmonella enterica serovar Enteritidis (S. Enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. The whole genome sequence analysis for S. Enteritidis unveiled the presence of several genetic regions that are absent in other Salmonella serovars. These regions have been denominated "regions of difference" (ROD). In this study we show that ROD21, one of such regions, behaves as an unstable pathogenicity island. We observed that ROD21 undergoes spontaneous excision by two independent recombination events, either under laboratory growth conditions or during infection of murine cells. Importantly, we also found that one type of excision occurred at higher rates when S. Enteritidis was residing inside murine phagocytic cells. These data suggest that ROD21 is an unstable pathogenicity island, whose frequency of excision depends on the environmental conditions found inside phagocytic cells.
format	article
author	Tania S Quiroz Pamela A Nieto Hugo E Tobar Francisco J Salazar-Echegarai Rodrigo J Lizana Carolina P Quezada Carlos A Santiviago Daniela V Araya Claudia A Riedel Alexis M Kalergis Susan M Bueno
author_facet	Tania S Quiroz Pamela A Nieto Hugo E Tobar Francisco J Salazar-Echegarai Rodrigo J Lizana Carolina P Quezada Carlos A Santiviago Daniela V Araya Claudia A Riedel Alexis M Kalergis Susan M Bueno
author_sort	Tania S Quiroz
title	Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.
title_short	Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.
title_full	Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.
title_fullStr	Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.
title_full_unstemmed	Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.
title_sort	excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/2f98ca31db9043c0ae02f2ce0f0471c7
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Excision of an unstable pathogenicity island in Salmonella enterica serovar Enteritidis is induced during infection of phagocytic cells.

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