Protective Effect of Catechin Hydrate on Spinal Cord Ischemia-reperfusion Injury in Rats
Background: Aortic artery stenosis leads to Ischemia-Reperfusion (I-R) injury, which can cause certain clinical expressions, such as paraplegia. Objectives: To appraise the effect of Catechin Hydrate (CH) against spinal cord I-R injury. Materials & Methods: A total of 35 male rats (250-300 g)...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Guilan University of Medical Sciences
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/2fa411e41f2d4035b6113081424868d9 |
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| Sumario: | Background: Aortic artery stenosis leads to Ischemia-Reperfusion (I-R) injury, which can cause certain clinical expressions, such as paraplegia.
Objectives: To appraise the effect of Catechin Hydrate (CH) against spinal cord I-R injury.
Materials & Methods: A total of 35 male rats (250-300 g) were divided randomly into five groups: intact, sham surgery, dimethyl sulfoxide (I-R+DMSO), low-dose CH (I-R+10 mg/kg CH), and high-dose CH (I-R+20 mg/kg CH). Abdominal aorta clamping was done for 60 min. Seventy-two hours after I-R, animals were evaluated for neurologic function, biochemical analysis, and histology. The data analysis was conducted by SPSS v. 16 using ANOVA and Kruskal-Wallis and Mann-Whitney U tests.
Results: The mean Motor Deficit Index (MDI) score and white matter damage in the CH (20 mg/kg) group were lower than in the DMSO group (P=0.032). The mean plasma levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the CH groups were lower than that of the DMSO group (P<0.05). The plasma level of Total Antioxidant Capacity (TAC) in the CH (20 mg/kg) group was higher than in the DMSO group (P=0.032). In addition, the plasma level of catalase in the CH (20 mg/kg) group was higher than in the DMSO and CH (10 mg/kg) groups (P<0.001). The average number of normal motor neurons in the experimental groups was lower than in the sham surgery group (P<0.001).
Conclusion: These results showed that CH may be effective in reducing spinal cord I-R injury. |
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