Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing

Abstract A suitcase laboratory was used for 16S rRNA amplicon sequencing to assess microbial water quality in the holy Bagmati River, Kathmandu, Nepal. SourceTracker analysis and Volcano plots revealed that microbial communities in the downstream part of the river were mainly contributed by untreate...

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Autores principales:	Kalyan Pantha, Kishor Acharya, Sanjeeb Mohapatra, Santosh Khanal, Niroj Amatya, Carolina Ospina-Betancourth, Giacomo Butte, Suman Dhun Shrestha, Prajwal Rajbhandari, David Werner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/2fa8820c34b34c029312b265fb1f787a
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spelling	oai:doaj.org-article:2fa8820c34b34c029312b265fb1f787a2021-12-02T14:03:43ZFaecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing10.1038/s41545-021-00099-12059-7037https://doaj.org/article/2fa8820c34b34c029312b265fb1f787a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41545-021-00099-1https://doaj.org/toc/2059-7037Abstract A suitcase laboratory was used for 16S rRNA amplicon sequencing to assess microbial water quality in the holy Bagmati River, Kathmandu, Nepal. SourceTracker analysis and Volcano plots revealed that microbial communities in the downstream part of the river were mainly contributed by untreated sewage. Seasonal variability in the sewage microbiome was reflected in the downstream river water quality. The bacterial genera Acidovorax, Geobacillus and Caulobacter predominated in the upstream sites, while genera containing putative human pathogens and gut bacteria, such as Clostridium, Prevotella, Arcobacter, Lactobacillus, Enterococcus and Streptococcus become prominent in the downstream sites. Marker gene qPCR assays for total bacteria, total coliforms, Human E. coli, Arcobacter butzleri and Vibrio cholerae confirmed the sequencing data trends. Even though basic sanitation provision is nowadays near universal in Nepal, our findings show how inadequate wastewater management may turn an urban river into an open sewer, which poses a public health risk.Kalyan PanthaKishor AcharyaSanjeeb MohapatraSantosh KhanalNiroj AmatyaCarolina Ospina-BetancourthGiacomo ButteSuman Dhun ShresthaPrajwal RajbhandariDavid WernerNature PortfolioarticleWater supply for domestic and industrial purposesTD201-500ENnpj Clean Water, Vol 4, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Water supply for domestic and industrial purposes TD201-500
spellingShingle	Water supply for domestic and industrial purposes TD201-500 Kalyan Pantha Kishor Acharya Sanjeeb Mohapatra Santosh Khanal Niroj Amatya Carolina Ospina-Betancourth Giacomo Butte Suman Dhun Shrestha Prajwal Rajbhandari David Werner Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
description	Abstract A suitcase laboratory was used for 16S rRNA amplicon sequencing to assess microbial water quality in the holy Bagmati River, Kathmandu, Nepal. SourceTracker analysis and Volcano plots revealed that microbial communities in the downstream part of the river were mainly contributed by untreated sewage. Seasonal variability in the sewage microbiome was reflected in the downstream river water quality. The bacterial genera Acidovorax, Geobacillus and Caulobacter predominated in the upstream sites, while genera containing putative human pathogens and gut bacteria, such as Clostridium, Prevotella, Arcobacter, Lactobacillus, Enterococcus and Streptococcus become prominent in the downstream sites. Marker gene qPCR assays for total bacteria, total coliforms, Human E. coli, Arcobacter butzleri and Vibrio cholerae confirmed the sequencing data trends. Even though basic sanitation provision is nowadays near universal in Nepal, our findings show how inadequate wastewater management may turn an urban river into an open sewer, which poses a public health risk.
format	article
author	Kalyan Pantha Kishor Acharya Sanjeeb Mohapatra Santosh Khanal Niroj Amatya Carolina Ospina-Betancourth Giacomo Butte Suman Dhun Shrestha Prajwal Rajbhandari David Werner
author_facet	Kalyan Pantha Kishor Acharya Sanjeeb Mohapatra Santosh Khanal Niroj Amatya Carolina Ospina-Betancourth Giacomo Butte Suman Dhun Shrestha Prajwal Rajbhandari David Werner
author_sort	Kalyan Pantha
title	Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
title_short	Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
title_full	Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
title_fullStr	Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
title_full_unstemmed	Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
title_sort	faecal pollution source tracking in the holy bagmati river by portable 16s rrna gene sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2fa8820c34b34c029312b265fb1f787a
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Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing

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