In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.

<h4>Objectives</h4>Thrombus and secondary thrombosis plays a key role in stroke. Recent molecular imaging provides in vivo imaging of activated factor XIII (FXIIIa), an important mediator of thrombosis or fibrinolytic resistance. The present study was to investigate the fibrin deposition...

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Autores principales:	Yi Zhang, Shufeng Fan, Yuyu Yao, Jie Ding, Yu Wang, Zhen Zhao, Lei Liao, Peicheng Li, Fengchao Zang, Gao-Jun Teng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2fc1e3dfe5704d03aab13f973d3a3f7d
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spelling	oai:doaj.org-article:2fc1e3dfe5704d03aab13f973d3a3f7d2021-11-18T07:30:06ZIn vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.1932-620310.1371/journal.pone.0030262https://doaj.org/article/2fc1e3dfe5704d03aab13f973d3a3f7d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22272319/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objectives</h4>Thrombus and secondary thrombosis plays a key role in stroke. Recent molecular imaging provides in vivo imaging of activated factor XIII (FXIIIa), an important mediator of thrombosis or fibrinolytic resistance. The present study was to investigate the fibrin deposition in a thromboembolic stroke mice model by FXIIIa-targeted near-infrared fluorescence (NIRF) imaging.<h4>Materials and methods</h4>The experimental protocol was approved by our institutional animal use committee. Seventy-six C57B/6J mice were subjected to thromboembolic middle cerebral artery occlusion or sham operation. Mice were either intravenously injected with the FXIIIa-targeted probe or control probe. In vivo and ex vivo NIRF imaging were performed thereafter. Probe distribution was assessed with fluorescence microscopy by spectral imaging and quantification system. MR scans were performed to measure lesion volumes in vivo, which were correlated with histology after animal euthanasia.<h4>Results</h4>In vivo significant higher fluorescence intensity over the ischemia-affected hemisphere, compared to the contralateral side, was detected in mice that received FXIIIa-targeted probe, but not in the controlled mice. Significantly NIRF signals showed time-dependent processes from 8 to 96 hours after injection of FXIIIa-targeted probes. Ex vivo NIRF image showed an intense fluorescence within the ischemic territory only in mice injected with FXIIIa-targeted probe. The fluorescence microscopy demonstrated distribution of FXIIIa-targeted probe in the ischemic region and nearby micro-vessels, and FXIIIa-targeted probe signals showed good overlap with immune-fluorescent fibrin staining images. There was a significant correlation between total targeted signal from in vivo or ex vivo NIRF images and lesion volume.<h4>Conclusion</h4>Non-invasive detection of fibrin deposition in ischemic mouse brain using NIRF imaging is feasible and this technique may provide an in vivo experimental tool in studying the role of fibrin in stroke.Yi ZhangShufeng FanYuyu YaoJie DingYu WangZhen ZhaoLei LiaoPeicheng LiFengchao ZangGao-Jun TengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30262 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Zhang Shufeng Fan Yuyu Yao Jie Ding Yu Wang Zhen Zhao Lei Liao Peicheng Li Fengchao Zang Gao-Jun Teng In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.
description	<h4>Objectives</h4>Thrombus and secondary thrombosis plays a key role in stroke. Recent molecular imaging provides in vivo imaging of activated factor XIII (FXIIIa), an important mediator of thrombosis or fibrinolytic resistance. The present study was to investigate the fibrin deposition in a thromboembolic stroke mice model by FXIIIa-targeted near-infrared fluorescence (NIRF) imaging.<h4>Materials and methods</h4>The experimental protocol was approved by our institutional animal use committee. Seventy-six C57B/6J mice were subjected to thromboembolic middle cerebral artery occlusion or sham operation. Mice were either intravenously injected with the FXIIIa-targeted probe or control probe. In vivo and ex vivo NIRF imaging were performed thereafter. Probe distribution was assessed with fluorescence microscopy by spectral imaging and quantification system. MR scans were performed to measure lesion volumes in vivo, which were correlated with histology after animal euthanasia.<h4>Results</h4>In vivo significant higher fluorescence intensity over the ischemia-affected hemisphere, compared to the contralateral side, was detected in mice that received FXIIIa-targeted probe, but not in the controlled mice. Significantly NIRF signals showed time-dependent processes from 8 to 96 hours after injection of FXIIIa-targeted probes. Ex vivo NIRF image showed an intense fluorescence within the ischemic territory only in mice injected with FXIIIa-targeted probe. The fluorescence microscopy demonstrated distribution of FXIIIa-targeted probe in the ischemic region and nearby micro-vessels, and FXIIIa-targeted probe signals showed good overlap with immune-fluorescent fibrin staining images. There was a significant correlation between total targeted signal from in vivo or ex vivo NIRF images and lesion volume.<h4>Conclusion</h4>Non-invasive detection of fibrin deposition in ischemic mouse brain using NIRF imaging is feasible and this technique may provide an in vivo experimental tool in studying the role of fibrin in stroke.
format	article
author	Yi Zhang Shufeng Fan Yuyu Yao Jie Ding Yu Wang Zhen Zhao Lei Liao Peicheng Li Fengchao Zang Gao-Jun Teng
author_facet	Yi Zhang Shufeng Fan Yuyu Yao Jie Ding Yu Wang Zhen Zhao Lei Liao Peicheng Li Fengchao Zang Gao-Jun Teng
author_sort	Yi Zhang
title	In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.
title_short	In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.
title_full	In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.
title_fullStr	In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.
title_full_unstemmed	In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.
title_sort	in vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2fc1e3dfe5704d03aab13f973d3a3f7d
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In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.

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