Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells

Modern therapeutic strategies for intervertebral disc repair mainly focus on targeting molecular pathways of extracellular matrix degeneration. Anabolic strategies for regeneration are aimed to increase production of major extracellular molecules. Members of TGF-b superfamily proteins, particularly...

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Autores principales:	L. A. Bardonova, E. G. Belykh, V. A. Byvaltsev
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	intervertebral disc degeneration bone morphogenetic protein proteoglycans Science Q
Acceso en línea:	https://doaj.org/article/2fc79432734c4c42af044574eaf9a657
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spelling	oai:doaj.org-article:2fc79432734c4c42af044574eaf9a6572021-11-23T06:14:35ZBone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells2541-94202587-959610.12737/22977https://doaj.org/article/2fc79432734c4c42af044574eaf9a6572016-07-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/250https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Modern therapeutic strategies for intervertebral disc repair mainly focus on targeting molecular pathways of extracellular matrix degeneration. Anabolic strategies for regeneration are aimed to increase production of major extracellular molecules. Members of TGF-b superfamily proteins, particularly the bone morphogenetic proteins (BMP) have a high regenerative potential regarding the mesenchymal cells. The goal of this study is to study production of proteoglycans by the intervertebral disc cells under the influence of bone morphogenetic protein 2. Material and methods. The experiment was carried out on the cell cultures derived from the annulus fibrosis cells and nucleus pulposus cells of the human intervertebral disc. We studied cell livability, metabolic activity and proteoglycan expression. Cell livability was assessed using the trypan blue staining. Alamar blue test was used for the estimation of metabolic activity. Amount of sulfated glycosaminoglycans was assessed using the assay based on the reaction with 1,9-Dimethylmethylene Blue. Results. Cultivation with bone morphogenetic protein 2 in different concentrations did not decrease livability of the cells. Study cell cultures with application of bone morphogenetic protein 2 in different concentrations showed significant increase in metabolic activity and proteoglycan synthesis by the annulus fibrosis cells. Despite the relative increase in the number of the nucleus pulposus cells treated with the bone morphogenetic protein 2, the differences in metabolic and synthetic activity compared with control group was not significant. Conclusion. The bone morphogenetic protein 2 has an anabolic effect towards the intervertebral disc cells, particularly in the production of proteoglycans by the annulus fibrosis cells.L. A. BardonovaE. G. BelykhV. A. ByvaltsevScientific Сentre for Family Health and Human Reproduction Problemsarticleintervertebral discdegenerationbone morphogenetic proteinproteoglycansScienceQRUActa Biomedica Scientifica, Vol 1, Iss 4, Pp 99-103 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	intervertebral disc degeneration bone morphogenetic protein proteoglycans Science Q
spellingShingle	intervertebral disc degeneration bone morphogenetic protein proteoglycans Science Q L. A. Bardonova E. G. Belykh V. A. Byvaltsev Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells
description	Modern therapeutic strategies for intervertebral disc repair mainly focus on targeting molecular pathways of extracellular matrix degeneration. Anabolic strategies for regeneration are aimed to increase production of major extracellular molecules. Members of TGF-b superfamily proteins, particularly the bone morphogenetic proteins (BMP) have a high regenerative potential regarding the mesenchymal cells. The goal of this study is to study production of proteoglycans by the intervertebral disc cells under the influence of bone morphogenetic protein 2. Material and methods. The experiment was carried out on the cell cultures derived from the annulus fibrosis cells and nucleus pulposus cells of the human intervertebral disc. We studied cell livability, metabolic activity and proteoglycan expression. Cell livability was assessed using the trypan blue staining. Alamar blue test was used for the estimation of metabolic activity. Amount of sulfated glycosaminoglycans was assessed using the assay based on the reaction with 1,9-Dimethylmethylene Blue. Results. Cultivation with bone morphogenetic protein 2 in different concentrations did not decrease livability of the cells. Study cell cultures with application of bone morphogenetic protein 2 in different concentrations showed significant increase in metabolic activity and proteoglycan synthesis by the annulus fibrosis cells. Despite the relative increase in the number of the nucleus pulposus cells treated with the bone morphogenetic protein 2, the differences in metabolic and synthetic activity compared with control group was not significant. Conclusion. The bone morphogenetic protein 2 has an anabolic effect towards the intervertebral disc cells, particularly in the production of proteoglycans by the annulus fibrosis cells.
format	article
author	L. A. Bardonova E. G. Belykh V. A. Byvaltsev
author_facet	L. A. Bardonova E. G. Belykh V. A. Byvaltsev
author_sort	L. A. Bardonova
title	Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells
title_short	Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells
title_full	Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells
title_fullStr	Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells
title_full_unstemmed	Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells
title_sort	bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/2fc79432734c4c42af044574eaf9a657
work_keys_str_mv	AT labardonova bonemorphogeneticprotein2influenceonmetabolicactivityandproteoglycansynthesisbyintervertebraldisccells AT egbelykh bonemorphogeneticprotein2influenceonmetabolicactivityandproteoglycansynthesisbyintervertebraldisccells AT vabyvaltsev bonemorphogeneticprotein2influenceonmetabolicactivityandproteoglycansynthesisbyintervertebraldisccells
_version_	1718417060650811392

Bone morphogenetic protein-2 influence on metabolic activity and proteoglycan synthesis by intervertebral disc cells

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