Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts

Abstract Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariam Y. El-Hattab, Yoshiaki Nagumo, Francoise A. Gourronc, Aloysius J. Klingelhutz, James A. Ankrum, Edward A. Sander
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2fcb313c25764006b3e282c1e69f58e8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2fcb313c25764006b3e282c1e69f58e8
record_format	dspace
spelling	oai:doaj.org-article:2fcb313c25764006b3e282c1e69f58e82021-12-02T17:45:04ZHuman Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts10.1038/s41598-020-67175-32045-2322https://doaj.org/article/2fcb313c25764006b3e282c1e69f58e82020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67175-3https://doaj.org/toc/2045-2322Abstract Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we investigated whether adipocytes and their precursors (pre-adipocytes) secrete factors that affect human dermal fibroblast behavior. We found that both adipocyte and pre-adipocyte conditioned medium induced the migration of fibroblasts, but only adipocyte conditioned medium induced fibroblast differentiation into a highly contractile, collagen producing myofibroblast phenotype. Furthermore, adipocyte mediated myofibroblast induction occurred through a TGF-β independent mechanism. Our findings contribute to a better understanding on the involvement of adipose tissue in wound healing, and may help to uncover and develop fat-related wound healing treatments.Mariam Y. El-HattabYoshiaki NagumoFrancoise A. GourroncAloysius J. KlingelhutzJames A. AnkrumEdward A. SanderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariam Y. El-Hattab Yoshiaki Nagumo Francoise A. Gourronc Aloysius J. Klingelhutz James A. Ankrum Edward A. Sander Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
description	Abstract Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we investigated whether adipocytes and their precursors (pre-adipocytes) secrete factors that affect human dermal fibroblast behavior. We found that both adipocyte and pre-adipocyte conditioned medium induced the migration of fibroblasts, but only adipocyte conditioned medium induced fibroblast differentiation into a highly contractile, collagen producing myofibroblast phenotype. Furthermore, adipocyte mediated myofibroblast induction occurred through a TGF-β independent mechanism. Our findings contribute to a better understanding on the involvement of adipose tissue in wound healing, and may help to uncover and develop fat-related wound healing treatments.
format	article
author	Mariam Y. El-Hattab Yoshiaki Nagumo Francoise A. Gourronc Aloysius J. Klingelhutz James A. Ankrum Edward A. Sander
author_facet	Mariam Y. El-Hattab Yoshiaki Nagumo Francoise A. Gourronc Aloysius J. Klingelhutz James A. Ankrum Edward A. Sander
author_sort	Mariam Y. El-Hattab
title	Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_short	Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_full	Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_fullStr	Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_full_unstemmed	Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_sort	human adipocyte conditioned medium promotes in vitro fibroblast conversion to myofibroblasts
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2fcb313c25764006b3e282c1e69f58e8
work_keys_str_mv	AT mariamyelhattab humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts AT yoshiakinagumo humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts AT francoiseagourronc humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts AT aloysiusjklingelhutz humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts AT jamesaankrum humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts AT edwardasander humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts
_version_	1718379577264308224

Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts

Ejemplares similares