PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI

Abstract Parahydrogen-induced polarization of 13C nuclei by side-arm hydrogenation (PHIP-SAH) for [1-13C]acetate and [1-13C]pyruvate esters with application of PH-INEPT-type pulse sequences for 1H to 13C polarization transfer is reported, and its efficiency is compared with that of polarization tran...

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Autores principales:	Alexandra Svyatova, Vitaly P. Kozinenko, Nikita V. Chukanov, Dudari B. Burueva, Eduard Y. Chekmenev, Yu-Wen Chen, Dennis W. Hwang, Kirill V. Kovtunov, Igor V. Koptyug
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2fcdf7748c404a658d390d7cb3f25b84
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spelling	oai:doaj.org-article:2fcdf7748c404a658d390d7cb3f25b842021-12-02T13:30:11ZPHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI10.1038/s41598-021-85136-22045-2322https://doaj.org/article/2fcdf7748c404a658d390d7cb3f25b842021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85136-2https://doaj.org/toc/2045-2322Abstract Parahydrogen-induced polarization of 13C nuclei by side-arm hydrogenation (PHIP-SAH) for [1-13C]acetate and [1-13C]pyruvate esters with application of PH-INEPT-type pulse sequences for 1H to 13C polarization transfer is reported, and its efficiency is compared with that of polarization transfer based on magnetic field cycling (MFC). The pulse-sequence transfer approach may have its merits in some applications because the entire hyperpolarization procedure is implemented directly in an NMR or MRI instrument, whereas MFC requires a controlled field variation at low magnetic fields. Optimization of the PH-INEPT-type transfer sequences resulted in 13C polarization values of 0.66 ± 0.04% and 0.19 ± 0.02% for allyl [1-13C]pyruvate and ethyl [1-13C]acetate, respectively, which is lower than the corresponding polarization levels obtained with MFC for 1H to 13C polarization transfer (3.95 ± 0.05% and 0.65 ± 0.05% for allyl [1-13C]pyruvate and ethyl [1-13C]acetate, respectively). Nevertheless, a significant 13C NMR signal enhancement with respect to thermal polarization allowed us to perform 13C MR imaging of both biologically relevant hyperpolarized molecules which can be used to produce useful contrast agents for the in vivo imaging applications.Alexandra SvyatovaVitaly P. KozinenkoNikita V. ChukanovDudari B. BuruevaEduard Y. ChekmenevYu-Wen ChenDennis W. HwangKirill V. KovtunovIgor V. KoptyugNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra Svyatova Vitaly P. Kozinenko Nikita V. Chukanov Dudari B. Burueva Eduard Y. Chekmenev Yu-Wen Chen Dennis W. Hwang Kirill V. Kovtunov Igor V. Koptyug PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI
description	Abstract Parahydrogen-induced polarization of 13C nuclei by side-arm hydrogenation (PHIP-SAH) for [1-13C]acetate and [1-13C]pyruvate esters with application of PH-INEPT-type pulse sequences for 1H to 13C polarization transfer is reported, and its efficiency is compared with that of polarization transfer based on magnetic field cycling (MFC). The pulse-sequence transfer approach may have its merits in some applications because the entire hyperpolarization procedure is implemented directly in an NMR or MRI instrument, whereas MFC requires a controlled field variation at low magnetic fields. Optimization of the PH-INEPT-type transfer sequences resulted in 13C polarization values of 0.66 ± 0.04% and 0.19 ± 0.02% for allyl [1-13C]pyruvate and ethyl [1-13C]acetate, respectively, which is lower than the corresponding polarization levels obtained with MFC for 1H to 13C polarization transfer (3.95 ± 0.05% and 0.65 ± 0.05% for allyl [1-13C]pyruvate and ethyl [1-13C]acetate, respectively). Nevertheless, a significant 13C NMR signal enhancement with respect to thermal polarization allowed us to perform 13C MR imaging of both biologically relevant hyperpolarized molecules which can be used to produce useful contrast agents for the in vivo imaging applications.
format	article
author	Alexandra Svyatova Vitaly P. Kozinenko Nikita V. Chukanov Dudari B. Burueva Eduard Y. Chekmenev Yu-Wen Chen Dennis W. Hwang Kirill V. Kovtunov Igor V. Koptyug
author_facet	Alexandra Svyatova Vitaly P. Kozinenko Nikita V. Chukanov Dudari B. Burueva Eduard Y. Chekmenev Yu-Wen Chen Dennis W. Hwang Kirill V. Kovtunov Igor V. Koptyug
author_sort	Alexandra Svyatova
title	PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI
title_short	PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI
title_full	PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI
title_fullStr	PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI
title_full_unstemmed	PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI
title_sort	phip hyperpolarized [1-13c]pyruvate and [1-13c]acetate esters via ph-inept polarization transfer monitored by 13c nmr and mri
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2fcdf7748c404a658d390d7cb3f25b84
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PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI

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