Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro

Franziska Koch,1–3 Anne Wolff,2 Stephanie Mathes,4 Uwe Pieles,1 Sina S Saxer,1 Bernd Kreikemeyer,3 Kirsten Peters2 1Institute for Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland; 2Department of Cel...

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Autores principales:	Koch F, Wolff A, Mathes S, Pieles U, Saxer SS, Kreikemeyer B, Peters K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Self-assembling peptides (SAPs) P11-SAP hydrogels surface charge protein adsorption cell proliferation osteogenic differentiation (periodontal) tissue regeneration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2fcfae9ac9a3481c8ced4becba2b845c
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spelling	oai:doaj.org-article:2fcfae9ac9a3481c8ced4becba2b845c2021-12-02T06:57:12ZAmino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro1178-2013https://doaj.org/article/2fcfae9ac9a3481c8ced4becba2b845c2018-10-01T00:00:00Zhttps://www.dovepress.com/amino-acid-composition-of-nanofibrillar-self-assembling-peptide-hydrog-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Franziska Koch,1–3 Anne Wolff,2 Stephanie Mathes,4 Uwe Pieles,1 Sina S Saxer,1 Bernd Kreikemeyer,3 Kirsten Peters2 1Institute for Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland; 2Department of Cell Biology, University Medicine Rostock, Rostock, Germany; 3Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany; 4Department for Chemistry and Biotechnology, Tissue Engineering, Zurich University of Applied Sciences, Wädenswil, Switzerland Background: The regeneration of tissue defects at the interface between soft and hard tissue, eg, in the periodontium, poses a challenge due to the divergent tissue requirements. A class of biomaterials that may support the regeneration at the soft-to-hard tissue interface are self-assembling peptides (SAPs), as their physicochemical and mechanical properties can be rationally designed to meet tissue requirements. Materials and methods: In this work, we investigated the effect of two single-component and two complementary β-sheet forming SAP systems on their hydrogel properties such as nanofibrillar architecture, surface charge, and protein adsorption as well as their influence on cell adhesion, morphology, growth, and differentiation. Results: We showed that these four 11-amino acid SAP (P11-SAP) hydrogels possessed physicochemical characteristics dependent on their amino acid composition that allowed variabilities in nanofibrillar network architecture, surface charge, and protein adsorption (eg, the single-component systems demonstrated an ~30% higher porosity and an almost 2-fold higher protein adsorption compared with the complementary systems). Cytocompatibility studies revealed similar results for cells cultured on the four P11-SAP hydrogels compared with cells on standard cell culture surfaces. The single-component P11-SAP systems showed a 1.7-fold increase in cell adhesion and cellular growth compared with the complementary P11-SAP systems. Moreover, significantly enhanced osteogenic differentiation of human calvarial osteoblasts was detected for the single-component P11-SAP system hydrogels compared with standard cell cultures. Conclusion: Thus, single-component system P11-SAP hydrogels can be assessed as suitable scaffolds for periodontal regeneration therapy, as they provide adjustable, extracellular matrix-mimetic nanofibrillar architecture and favorable cellular interaction with periodontal cells. Keywords: self-assembling peptides, SAPs, P11-SAP hydrogels, surface charge, protein adsorption, cell proliferation, osteogenic differentiation, periodontal tissue regenerationKoch FWolff AMathes SPieles USaxer SSKreikemeyer BPeters KDove Medical PressarticleSelf-assembling peptides (SAPs)P11-SAP hydrogelssurface chargeprotein adsorptioncell proliferationosteogenic differentiation(periodontal) tissue regenerationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6717-6733 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Self-assembling peptides (SAPs) P11-SAP hydrogels surface charge protein adsorption cell proliferation osteogenic differentiation (periodontal) tissue regeneration Medicine (General) R5-920
spellingShingle	Self-assembling peptides (SAPs) P11-SAP hydrogels surface charge protein adsorption cell proliferation osteogenic differentiation (periodontal) tissue regeneration Medicine (General) R5-920 Koch F Wolff A Mathes S Pieles U Saxer SS Kreikemeyer B Peters K Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro
description	Franziska Koch,1–3 Anne Wolff,2 Stephanie Mathes,4 Uwe Pieles,1 Sina S Saxer,1 Bernd Kreikemeyer,3 Kirsten Peters2 1Institute for Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland; 2Department of Cell Biology, University Medicine Rostock, Rostock, Germany; 3Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany; 4Department for Chemistry and Biotechnology, Tissue Engineering, Zurich University of Applied Sciences, Wädenswil, Switzerland Background: The regeneration of tissue defects at the interface between soft and hard tissue, eg, in the periodontium, poses a challenge due to the divergent tissue requirements. A class of biomaterials that may support the regeneration at the soft-to-hard tissue interface are self-assembling peptides (SAPs), as their physicochemical and mechanical properties can be rationally designed to meet tissue requirements. Materials and methods: In this work, we investigated the effect of two single-component and two complementary β-sheet forming SAP systems on their hydrogel properties such as nanofibrillar architecture, surface charge, and protein adsorption as well as their influence on cell adhesion, morphology, growth, and differentiation. Results: We showed that these four 11-amino acid SAP (P11-SAP) hydrogels possessed physicochemical characteristics dependent on their amino acid composition that allowed variabilities in nanofibrillar network architecture, surface charge, and protein adsorption (eg, the single-component systems demonstrated an ~30% higher porosity and an almost 2-fold higher protein adsorption compared with the complementary systems). Cytocompatibility studies revealed similar results for cells cultured on the four P11-SAP hydrogels compared with cells on standard cell culture surfaces. The single-component P11-SAP systems showed a 1.7-fold increase in cell adhesion and cellular growth compared with the complementary P11-SAP systems. Moreover, significantly enhanced osteogenic differentiation of human calvarial osteoblasts was detected for the single-component P11-SAP system hydrogels compared with standard cell cultures. Conclusion: Thus, single-component system P11-SAP hydrogels can be assessed as suitable scaffolds for periodontal regeneration therapy, as they provide adjustable, extracellular matrix-mimetic nanofibrillar architecture and favorable cellular interaction with periodontal cells. Keywords: self-assembling peptides, SAPs, P11-SAP hydrogels, surface charge, protein adsorption, cell proliferation, osteogenic differentiation, periodontal tissue regeneration
format	article
author	Koch F Wolff A Mathes S Pieles U Saxer SS Kreikemeyer B Peters K
author_facet	Koch F Wolff A Mathes S Pieles U Saxer SS Kreikemeyer B Peters K
author_sort	Koch F
title	Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro
title_short	Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro
title_full	Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro
title_fullStr	Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro
title_full_unstemmed	Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro
title_sort	amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/2fcfae9ac9a3481c8ced4becba2b845c
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Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro

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