Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms

Hao Guo,1 Ziming Wang,1 Quanyin Du,1 Pan Li,2 Zhigang Wang,2 Aimin Wang1 1Department of Orthopedics, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China; 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, Institute of Ultrasound Imaging, Secon...

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Autores principales:	Guo H, Wang Z, Du Q, Li P, Wang A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	nanoscale droplets MRSA biofilm matrix ultrasound phase change cavitation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2fd67ed025f14e5b896d6fe7d2adf1ea
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spelling	oai:doaj.org-article:2fd67ed025f14e5b896d6fe7d2adf1ea2021-12-02T05:36:20ZStimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms1178-2013https://doaj.org/article/2fd67ed025f14e5b896d6fe7d2adf1ea2017-06-01T00:00:00Zhttps://www.dovepress.com/stimulated-phase-shift-acoustic-nanodroplets-enhance-vancomycin-effica-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hao Guo,1 Ziming Wang,1 Quanyin Du,1 Pan Li,2 Zhigang Wang,2 Aimin Wang1 1Department of Orthopedics, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China; 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China Purpose: Bacterial biofilms on the surface of prostheses are becoming a rising concern in managing prosthetic joint infections. The inherent resistant features of biofilms render traditional antimicrobial therapy unproductive and revision surgery outcomes uncertain. This situation has prompted the exploration of novel antimicrobial strategies. The synergy of ultrasound microbubbles and vancomycin has been proposed as an efficient alternative for biofilm eradication. The purpose of this study was to evaluate the anti-biofilm effect of stimulated phase-shift acoustic nanodroplets (NDs) combined with vancomycin.Materials and methods: We fabricated lipid phase-shift NDs with a core of liquid perfluoropentane. A new phase change mode for NDs incorporating an initial unfocused low-intensity pulsed ultrasound for 5 minutes and a subsequent incubation at 37°C into a 24-hour duration was developed. Methicillin-resistant Staphylococcus aureus (MRSA) biofilms were incubated with vancomycin and NDs under the hybrid stimulation. Biofilm morphology following treatment was determined using confocal laser scanning microscopy and scanning electron microscopy. Resazurin assay was used to quantify bactericidal efficacy against MRSA biofilm bacteria.Results: NDs treated sequentially with ultrasound and heating at 37°C achieved gradual and substantial ND vaporization and cavitation in a successive process. NDs after stimulation were capable of generating stronger destruction on biofilm structure which was best characterized by residual circular arc margins and more dead bacteria. Furthermore, NDs combined with vancomycin contributed to significantly decreasing the metabolic activity of bacteria in MRSA biofilms (P<0.05).Conclusion: Phase-shift acoustic NDs could exert a significant bactericidal effect against MRSA biofilms through a new stimulation mode. Acoustic NDs present advantages over microbubbles for biofilm damage. This anti-biofilm strategy could be used either alone or as an enhancer of traditional antibiotics in the control of prosthetic joint infections. Keywords: nanodroplets, MRSA, biofilm matrix, ultrasound, phase change, cavitationGuo HWang ZDu QLi PWang ZWang ADove Medical Pressarticlenanoscale dropletsMRSAbiofilm matrixultrasoundphase changecavitationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4679-4690 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoscale droplets MRSA biofilm matrix ultrasound phase change cavitation Medicine (General) R5-920
spellingShingle	nanoscale droplets MRSA biofilm matrix ultrasound phase change cavitation Medicine (General) R5-920 Guo H Wang Z Du Q Li P Wang Z Wang A Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms
description	Hao Guo,1 Ziming Wang,1 Quanyin Du,1 Pan Li,2 Zhigang Wang,2 Aimin Wang1 1Department of Orthopedics, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China; 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China Purpose: Bacterial biofilms on the surface of prostheses are becoming a rising concern in managing prosthetic joint infections. The inherent resistant features of biofilms render traditional antimicrobial therapy unproductive and revision surgery outcomes uncertain. This situation has prompted the exploration of novel antimicrobial strategies. The synergy of ultrasound microbubbles and vancomycin has been proposed as an efficient alternative for biofilm eradication. The purpose of this study was to evaluate the anti-biofilm effect of stimulated phase-shift acoustic nanodroplets (NDs) combined with vancomycin.Materials and methods: We fabricated lipid phase-shift NDs with a core of liquid perfluoropentane. A new phase change mode for NDs incorporating an initial unfocused low-intensity pulsed ultrasound for 5 minutes and a subsequent incubation at 37°C into a 24-hour duration was developed. Methicillin-resistant Staphylococcus aureus (MRSA) biofilms were incubated with vancomycin and NDs under the hybrid stimulation. Biofilm morphology following treatment was determined using confocal laser scanning microscopy and scanning electron microscopy. Resazurin assay was used to quantify bactericidal efficacy against MRSA biofilm bacteria.Results: NDs treated sequentially with ultrasound and heating at 37°C achieved gradual and substantial ND vaporization and cavitation in a successive process. NDs after stimulation were capable of generating stronger destruction on biofilm structure which was best characterized by residual circular arc margins and more dead bacteria. Furthermore, NDs combined with vancomycin contributed to significantly decreasing the metabolic activity of bacteria in MRSA biofilms (P<0.05).Conclusion: Phase-shift acoustic NDs could exert a significant bactericidal effect against MRSA biofilms through a new stimulation mode. Acoustic NDs present advantages over microbubbles for biofilm damage. This anti-biofilm strategy could be used either alone or as an enhancer of traditional antibiotics in the control of prosthetic joint infections. Keywords: nanodroplets, MRSA, biofilm matrix, ultrasound, phase change, cavitation
format	article
author	Guo H Wang Z Du Q Li P Wang Z Wang A
author_facet	Guo H Wang Z Du Q Li P Wang Z Wang A
author_sort	Guo H
title	Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms
title_short	Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms
title_full	Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms
title_fullStr	Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms
title_full_unstemmed	Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms
title_sort	stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant staphylococcus aureus biofilms
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/2fd67ed025f14e5b896d6fe7d2adf1ea
work_keys_str_mv	AT guoh stimulatedphaseshiftacousticnanodropletsenhancevancomycinefficacyagainstmethicillinresistantstaphylococcusaureusbiofilms AT wangz stimulatedphaseshiftacousticnanodropletsenhancevancomycinefficacyagainstmethicillinresistantstaphylococcusaureusbiofilms AT duq stimulatedphaseshiftacousticnanodropletsenhancevancomycinefficacyagainstmethicillinresistantstaphylococcusaureusbiofilms AT lip stimulatedphaseshiftacousticnanodropletsenhancevancomycinefficacyagainstmethicillinresistantstaphylococcusaureusbiofilms AT wangz stimulatedphaseshiftacousticnanodropletsenhancevancomycinefficacyagainstmethicillinresistantstaphylococcusaureusbiofilms AT wanga stimulatedphaseshiftacousticnanodropletsenhancevancomycinefficacyagainstmethicillinresistantstaphylococcusaureusbiofilms
_version_	1718400286373969920

Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms

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