LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex

Gastric cancer (GC) is one of the deadliest cancers, and long noncoding RNAs (lncRNAs) have been reported to be the important regulators during the occurrence and development of GC. The present study identified a novel and functional lncRNA in GC, named NR038975, which was confirmed to be markedly u...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sisi Wei, Suli Dai, Cong Zhang, Ruinian Zhao, Zitong Zhao, Yongmei Song, Baoen Shan, Lianmei Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	gastric cancer lncRNA NR038975 NF90/NF45 tumorigenesis exosome Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/2fde21c3b1ce42cd953714476603b971
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2fde21c3b1ce42cd953714476603b971
record_format	dspace
spelling	oai:doaj.org-article:2fde21c3b1ce42cd953714476603b9712021-11-17T04:27:31ZLncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex2234-943X10.3389/fonc.2021.721604https://doaj.org/article/2fde21c3b1ce42cd953714476603b9712021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.721604/fullhttps://doaj.org/toc/2234-943XGastric cancer (GC) is one of the deadliest cancers, and long noncoding RNAs (lncRNAs) have been reported to be the important regulators during the occurrence and development of GC. The present study identified a novel and functional lncRNA in GC, named NR038975, which was confirmed to be markedly upregulated in the Gene Expression Profiling Interactive Analysis (GEPIA) dataset and our independent cohort of GC tissues. We firstly characterized the full-length sequence and subcellular location of NR038975 in GC cells. Our data demonstrated that upregulated NR038975 expression was significantly related to lymph node metastasis and TNM stage. In addition, knockdown of NR038975 inhibited GC cell proliferation, migration, invasion, and clonogenicity and vice versa. Mechanistically, RNA pull-down and mass spectrometry assays identified the NR038975-binding proteins and NF90/NF45 complex, and the binding was also confirmed by RNA immunoprecipitation and confocal experiments. We further demonstrated that genetic deficiency of NR038975 abrogated the interaction between NF45 and NF90. Moreover, NF90 increased the stability of NR038975. Thus, NR038975-NF90/NF45 will be an important combinational target of GC. Finally, we detected NR038975 in serum exosomes and serum of GC patients. Our results indicated that NR038975 was a biomarker for gastric tumorigenesis. The current study demonstrated that NR038975 is a novel lncRNA that is clinically and functionally engaged in GC progression and might be a novel diagnostic marker and potential therapeutic target.Sisi WeiSuli DaiCong ZhangRuinian ZhaoZitong ZhaoYongmei SongBaoen ShanLianmei ZhaoFrontiers Media S.A.articlegastric cancerlncRNA NR038975NF90/NF45tumorigenesisexosomeNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gastric cancer lncRNA NR038975 NF90/NF45 tumorigenesis exosome Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	gastric cancer lncRNA NR038975 NF90/NF45 tumorigenesis exosome Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Sisi Wei Suli Dai Cong Zhang Ruinian Zhao Zitong Zhao Yongmei Song Baoen Shan Lianmei Zhao LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex
description	Gastric cancer (GC) is one of the deadliest cancers, and long noncoding RNAs (lncRNAs) have been reported to be the important regulators during the occurrence and development of GC. The present study identified a novel and functional lncRNA in GC, named NR038975, which was confirmed to be markedly upregulated in the Gene Expression Profiling Interactive Analysis (GEPIA) dataset and our independent cohort of GC tissues. We firstly characterized the full-length sequence and subcellular location of NR038975 in GC cells. Our data demonstrated that upregulated NR038975 expression was significantly related to lymph node metastasis and TNM stage. In addition, knockdown of NR038975 inhibited GC cell proliferation, migration, invasion, and clonogenicity and vice versa. Mechanistically, RNA pull-down and mass spectrometry assays identified the NR038975-binding proteins and NF90/NF45 complex, and the binding was also confirmed by RNA immunoprecipitation and confocal experiments. We further demonstrated that genetic deficiency of NR038975 abrogated the interaction between NF45 and NF90. Moreover, NF90 increased the stability of NR038975. Thus, NR038975-NF90/NF45 will be an important combinational target of GC. Finally, we detected NR038975 in serum exosomes and serum of GC patients. Our results indicated that NR038975 was a biomarker for gastric tumorigenesis. The current study demonstrated that NR038975 is a novel lncRNA that is clinically and functionally engaged in GC progression and might be a novel diagnostic marker and potential therapeutic target.
format	article
author	Sisi Wei Suli Dai Cong Zhang Ruinian Zhao Zitong Zhao Yongmei Song Baoen Shan Lianmei Zhao
author_facet	Sisi Wei Suli Dai Cong Zhang Ruinian Zhao Zitong Zhao Yongmei Song Baoen Shan Lianmei Zhao
author_sort	Sisi Wei
title	LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex
title_short	LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex
title_full	LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex
title_fullStr	LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex
title_full_unstemmed	LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex
title_sort	lncrna nr038975, a serum-based biomarker, promotes gastric tumorigenesis by interacting with nf90/nf45 complex
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2fde21c3b1ce42cd953714476603b971
work_keys_str_mv	AT sisiwei lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex AT sulidai lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex AT congzhang lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex AT ruinianzhao lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex AT zitongzhao lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex AT yongmeisong lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex AT baoenshan lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex AT lianmeizhao lncrnanr038975aserumbasedbiomarkerpromotesgastrictumorigenesisbyinteractingwithnf90nf45complex
_version_	1718425979265744896

LncRNA NR038975, A Serum-Based Biomarker, Promotes Gastric Tumorigenesis by Interacting With NF90/NF45 Complex

Ejemplares similares