Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals

Rice grass has been reported to contain bioactive compounds that possess antioxidant and free-radical scavenging activities. We aimed to assess rice grass extract (RGE) drink by determining catechin content, free-radical scavenging and iron-binding properties, as well as toxicity in cells and animal...

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Autores principales:	Suthaya Phimphilai, Pimpisid Koonyosying, Nuntouchaporn Hutachok, Tanyaluk Kampoun, Rufus Daw, Chaiyavat Chaiyasut, Vanli Prasartthong-osoth, Somdet Srichairatanakool
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidant catechins iron-chelating <i>Oryza sativa</i> rice toxicity Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/2fe1dd6d820f4dd49c6bcfe116978c49
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spelling	oai:doaj.org-article:2fe1dd6d820f4dd49c6bcfe116978c492021-11-25T18:28:09ZIdentifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals10.3390/molecules262268871420-3049https://doaj.org/article/2fe1dd6d820f4dd49c6bcfe116978c492021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6887https://doaj.org/toc/1420-3049Rice grass has been reported to contain bioactive compounds that possess antioxidant and free-radical scavenging activities. We aimed to assess rice grass extract (RGE) drink by determining catechin content, free-radical scavenging and iron-binding properties, as well as toxicity in cells and animals. Young rice grass (Sukhothai-1 strain) was dried, extracted with hot water and lyophilized in a vacuum chamber. The resulting extract was reconstituted with deionized water (260 mg/40 mL) and served as Sukhothai-1 rice grass extract drink (ST1-RGE). HPLC results revealed at least eight phenolic compounds, for which the major catechins were catechin, epicatechin and epigallocatechin-3-gallate (EGCG) (2.71–3.57, 0.98–1.85 and 25.47–27.55 mg/40 mL serving, respectively). Elements (As, Cu, Pb, Sn and Zn) and aflatoxin (B1, B2, G1 and G2) contents did not exceed the relevant limits when compared with WHO guideline values. Importantly, ST1-RGE drink exerted radical-scavenging, iron-chelating and anti-lipid peroxidation properties in aqueous and biological environments in a concentration-dependent manner. The drink was not toxic to cells and animals. Thus, Sukhothai-1 rice grass product is an edible drink that is rich in catechins, particularly EGCG, and exhibited antioxidant, free radical scavenging and iron-binding/chelating properties. The product represents a functional drink that is capable of alleviating conditions of oxidative stress and iron overload.Suthaya PhimphilaiPimpisid KoonyosyingNuntouchaporn HutachokTanyaluk KampounRufus DawChaiyavat ChaiyasutVanli Prasartthong-osothSomdet SrichairatanakoolMDPI AGarticleantioxidantcatechinsiron-chelating<i>Oryza sativa</i>ricetoxicityOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6887, p 6887 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant catechins iron-chelating <i>Oryza sativa</i> rice toxicity Organic chemistry QD241-441
spellingShingle	antioxidant catechins iron-chelating <i>Oryza sativa</i> rice toxicity Organic chemistry QD241-441 Suthaya Phimphilai Pimpisid Koonyosying Nuntouchaporn Hutachok Tanyaluk Kampoun Rufus Daw Chaiyavat Chaiyasut Vanli Prasartthong-osoth Somdet Srichairatanakool Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals
description	Rice grass has been reported to contain bioactive compounds that possess antioxidant and free-radical scavenging activities. We aimed to assess rice grass extract (RGE) drink by determining catechin content, free-radical scavenging and iron-binding properties, as well as toxicity in cells and animals. Young rice grass (Sukhothai-1 strain) was dried, extracted with hot water and lyophilized in a vacuum chamber. The resulting extract was reconstituted with deionized water (260 mg/40 mL) and served as Sukhothai-1 rice grass extract drink (ST1-RGE). HPLC results revealed at least eight phenolic compounds, for which the major catechins were catechin, epicatechin and epigallocatechin-3-gallate (EGCG) (2.71–3.57, 0.98–1.85 and 25.47–27.55 mg/40 mL serving, respectively). Elements (As, Cu, Pb, Sn and Zn) and aflatoxin (B1, B2, G1 and G2) contents did not exceed the relevant limits when compared with WHO guideline values. Importantly, ST1-RGE drink exerted radical-scavenging, iron-chelating and anti-lipid peroxidation properties in aqueous and biological environments in a concentration-dependent manner. The drink was not toxic to cells and animals. Thus, Sukhothai-1 rice grass product is an edible drink that is rich in catechins, particularly EGCG, and exhibited antioxidant, free radical scavenging and iron-binding/chelating properties. The product represents a functional drink that is capable of alleviating conditions of oxidative stress and iron overload.
format	article
author	Suthaya Phimphilai Pimpisid Koonyosying Nuntouchaporn Hutachok Tanyaluk Kampoun Rufus Daw Chaiyavat Chaiyasut Vanli Prasartthong-osoth Somdet Srichairatanakool
author_facet	Suthaya Phimphilai Pimpisid Koonyosying Nuntouchaporn Hutachok Tanyaluk Kampoun Rufus Daw Chaiyavat Chaiyasut Vanli Prasartthong-osoth Somdet Srichairatanakool
author_sort	Suthaya Phimphilai
title	Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals
title_short	Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals
title_full	Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals
title_fullStr	Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals
title_full_unstemmed	Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals
title_sort	identifying chemical composition, safety and bioactivity of thai rice grass extract drink in cells and animals
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2fe1dd6d820f4dd49c6bcfe116978c49
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Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals

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