Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

Biological control is a promising approach to suppress diseases caused by Pythium spp. such as Pythium soft rot of ginger caused by P. myriotylum. Unusually for a single genus, it also includes species that can antagonize Pythium plant pathogens, such as Pythium oligandrum. We investigated if a new...

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Autores principales:	Paul Daly, Siqiao Chen, Taiqiang Xue, Jingjing Li, Taha Majid Mahmood Sheikh, Qimeng Zhang, Xuehai Wang, Jinfeng Zhang, David A. Fitzpatrick, Jamie McGowan, Xiujuan Shi, Sheng Deng, Min Jiu, Dongmei Zhou, Irina S. Druzhinina, Lihui Wei
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Pythium oligandrum bioeffector dual RNAseq antagonism ginger Pythium myriotylum Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2fe9b8c4b1d9439a94e14e8dac9e75dd
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spelling	oai:doaj.org-article:2fe9b8c4b1d9439a94e14e8dac9e75dd2021-11-16T07:46:45ZDual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum1664-302X10.3389/fmicb.2021.765872https://doaj.org/article/2fe9b8c4b1d9439a94e14e8dac9e75dd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.765872/fullhttps://doaj.org/toc/1664-302XBiological control is a promising approach to suppress diseases caused by Pythium spp. such as Pythium soft rot of ginger caused by P. myriotylum. Unusually for a single genus, it also includes species that can antagonize Pythium plant pathogens, such as Pythium oligandrum. We investigated if a new isolate of P. oligandrum could antagonize P. myriotylum, what changes occurred in gene expression when P. oligandrum (antagonist) and P. myriotylum (host) interacted, and whether P. oligandrum could control soft-rot of ginger caused by P. myriotylum. An isolate of P. oligandrum, GAQ1, recovered from soil could antagonize P. myriotylum in a plate-based confrontation assay whereby P. myriotylum became non-viable. The loss of viability of P. myriotylum coupled with how P. oligandrum hyphae could coil around and penetrate the hyphae of P. myriotylum, indicated a predatory interaction. We investigated the transcriptional responses of P. myriotylum and P. oligandrum using dual-RNAseq at a stage in the confrontation where similar levels of total transcripts were measured from each species. As part of the transcriptional response of P. myriotylum to the presence of P. oligandrum, genes including a subset of putative Kazal-type protease inhibitors were strongly upregulated along with cellulases, elicitin-like proteins and genes involved in the repair of DNA double-strand breaks. In P. oligandrum, proteases, cellulases, and peroxidases featured prominently in the upregulated genes. The upregulation along with constitutive expression of P. oligandrum proteases appeared to be responded to by the upregulation of putative protease inhibitors from P. myriotylum, suggesting a P. myriotylum defensive strategy. Notwithstanding this P. myriotylum defensive strategy, P. oligandrum had a strong disease control effect on soft-rot of ginger caused by P. myriotylum. The newly isolated strain of P. oligandrum is a promising biocontrol agent for suppressing the soft-rot of ginger. The dual-RNAseq approach highlights responses of P. myriotylum that suggests features of a defensive strategy, and are perhaps another factor that may contribute to the variable success and durability of biological attempts to control diseases caused by Pythium spp.Paul DalySiqiao ChenSiqiao ChenTaiqiang XueJingjing LiTaha Majid Mahmood SheikhQimeng ZhangXuehai WangJinfeng ZhangDavid A. FitzpatrickJamie McGowanXiujuan ShiSheng DengMin JiuDongmei ZhouIrina S. DruzhininaLihui WeiLihui WeiFrontiers Media S.A.articlePythium oligandrumbioeffectordual RNAseqantagonismgingerPythium myriotylumMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pythium oligandrum bioeffector dual RNAseq antagonism ginger Pythium myriotylum Microbiology QR1-502
spellingShingle	Pythium oligandrum bioeffector dual RNAseq antagonism ginger Pythium myriotylum Microbiology QR1-502 Paul Daly Siqiao Chen Siqiao Chen Taiqiang Xue Jingjing Li Taha Majid Mahmood Sheikh Qimeng Zhang Xuehai Wang Jinfeng Zhang David A. Fitzpatrick Jamie McGowan Xiujuan Shi Sheng Deng Min Jiu Dongmei Zhou Irina S. Druzhinina Lihui Wei Lihui Wei Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum
description	Biological control is a promising approach to suppress diseases caused by Pythium spp. such as Pythium soft rot of ginger caused by P. myriotylum. Unusually for a single genus, it also includes species that can antagonize Pythium plant pathogens, such as Pythium oligandrum. We investigated if a new isolate of P. oligandrum could antagonize P. myriotylum, what changes occurred in gene expression when P. oligandrum (antagonist) and P. myriotylum (host) interacted, and whether P. oligandrum could control soft-rot of ginger caused by P. myriotylum. An isolate of P. oligandrum, GAQ1, recovered from soil could antagonize P. myriotylum in a plate-based confrontation assay whereby P. myriotylum became non-viable. The loss of viability of P. myriotylum coupled with how P. oligandrum hyphae could coil around and penetrate the hyphae of P. myriotylum, indicated a predatory interaction. We investigated the transcriptional responses of P. myriotylum and P. oligandrum using dual-RNAseq at a stage in the confrontation where similar levels of total transcripts were measured from each species. As part of the transcriptional response of P. myriotylum to the presence of P. oligandrum, genes including a subset of putative Kazal-type protease inhibitors were strongly upregulated along with cellulases, elicitin-like proteins and genes involved in the repair of DNA double-strand breaks. In P. oligandrum, proteases, cellulases, and peroxidases featured prominently in the upregulated genes. The upregulation along with constitutive expression of P. oligandrum proteases appeared to be responded to by the upregulation of putative protease inhibitors from P. myriotylum, suggesting a P. myriotylum defensive strategy. Notwithstanding this P. myriotylum defensive strategy, P. oligandrum had a strong disease control effect on soft-rot of ginger caused by P. myriotylum. The newly isolated strain of P. oligandrum is a promising biocontrol agent for suppressing the soft-rot of ginger. The dual-RNAseq approach highlights responses of P. myriotylum that suggests features of a defensive strategy, and are perhaps another factor that may contribute to the variable success and durability of biological attempts to control diseases caused by Pythium spp.
format	article
author	Paul Daly Siqiao Chen Siqiao Chen Taiqiang Xue Jingjing Li Taha Majid Mahmood Sheikh Qimeng Zhang Xuehai Wang Jinfeng Zhang David A. Fitzpatrick Jamie McGowan Xiujuan Shi Sheng Deng Min Jiu Dongmei Zhou Irina S. Druzhinina Lihui Wei Lihui Wei
author_facet	Paul Daly Siqiao Chen Siqiao Chen Taiqiang Xue Jingjing Li Taha Majid Mahmood Sheikh Qimeng Zhang Xuehai Wang Jinfeng Zhang David A. Fitzpatrick Jamie McGowan Xiujuan Shi Sheng Deng Min Jiu Dongmei Zhou Irina S. Druzhinina Lihui Wei Lihui Wei
author_sort	Paul Daly
title	Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum
title_short	Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum
title_full	Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum
title_fullStr	Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum
title_full_unstemmed	Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum
title_sort	dual-transcriptomic, microscopic, and biocontrol analyses of the interaction between the bioeffector pythium oligandrum and the pythium soft-rot of ginger pathogen pythium myriotylum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2fe9b8c4b1d9439a94e14e8dac9e75dd
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Dual-Transcriptomic, Microscopic, and Biocontrol Analyses of the Interaction Between the Bioeffector Pythium oligandrum and the Pythium Soft-Rot of Ginger Pathogen Pythium myriotylum

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