Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction

Abstract Escherichia coli is both a harmless commensal in the intestines of many mammals, as well as a dangerous pathogen. The evolutionary paths taken by strains of this species in the commensal-to-pathogen transition are complex and can involve changes both in the core genome, as well in the pan-g...

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Autores principales:	João T. Proença, Duarte C. Barral, Isabel Gordo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2feb55e305f543d4ae03a8c8c92fefd4
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spelling	oai:doaj.org-article:2feb55e305f543d4ae03a8c8c92fefd42021-12-02T16:06:42ZCommensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction10.1038/s41598-017-04081-12045-2322https://doaj.org/article/2feb55e305f543d4ae03a8c8c92fefd42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04081-1https://doaj.org/toc/2045-2322Abstract Escherichia coli is both a harmless commensal in the intestines of many mammals, as well as a dangerous pathogen. The evolutionary paths taken by strains of this species in the commensal-to-pathogen transition are complex and can involve changes both in the core genome, as well in the pan-genome. One way to understand the likely paths that a commensal strain of E. coli takes when evolving pathogenicity is through experimentally evolving the strain under the selective pressures that it will have to withstand as a pathogen. Here, we report that a commensal strain, under continuous pressure from macrophages, recurrently acquired a transposable element insertion, which resulted in two key phenotypic changes: increased intracellular survival, through the delay of phagosome maturation and increased ability to escape macrophages. We further show that the acquisition of the pathoadaptive traits was accompanied by small but significant changes in the transcriptome of macrophages upon infection. These results show that under constant pressures from a key component of the host immune system, namely macrophage phagocytosis, commensal E. coli rapidly acquires pathoadaptive mutations that cause transcriptome changes associated to the host-microbe duet.João T. ProençaDuarte C. BarralIsabel GordoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q João T. Proença Duarte C. Barral Isabel Gordo Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction
description	Abstract Escherichia coli is both a harmless commensal in the intestines of many mammals, as well as a dangerous pathogen. The evolutionary paths taken by strains of this species in the commensal-to-pathogen transition are complex and can involve changes both in the core genome, as well in the pan-genome. One way to understand the likely paths that a commensal strain of E. coli takes when evolving pathogenicity is through experimentally evolving the strain under the selective pressures that it will have to withstand as a pathogen. Here, we report that a commensal strain, under continuous pressure from macrophages, recurrently acquired a transposable element insertion, which resulted in two key phenotypic changes: increased intracellular survival, through the delay of phagosome maturation and increased ability to escape macrophages. We further show that the acquisition of the pathoadaptive traits was accompanied by small but significant changes in the transcriptome of macrophages upon infection. These results show that under constant pressures from a key component of the host immune system, namely macrophage phagocytosis, commensal E. coli rapidly acquires pathoadaptive mutations that cause transcriptome changes associated to the host-microbe duet.
format	article
author	João T. Proença Duarte C. Barral Isabel Gordo
author_facet	João T. Proença Duarte C. Barral Isabel Gordo
author_sort	João T. Proença
title	Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction
title_short	Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction
title_full	Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction
title_fullStr	Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction
title_full_unstemmed	Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction
title_sort	commensal-to-pathogen transition: one-single transposon insertion results in two pathoadaptive traits in escherichia coli -macrophage interaction
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2feb55e305f543d4ae03a8c8c92fefd4
work_keys_str_mv	AT joaotproenca commensaltopathogentransitiononesingletransposoninsertionresultsintwopathoadaptivetraitsinescherichiacolimacrophageinteraction AT duartecbarral commensaltopathogentransitiononesingletransposoninsertionresultsintwopathoadaptivetraitsinescherichiacolimacrophageinteraction AT isabelgordo commensaltopathogentransitiononesingletransposoninsertionresultsintwopathoadaptivetraitsinescherichiacolimacrophageinteraction
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Commensal-to-pathogen transition: One-single transposon insertion results in two pathoadaptive traits in Escherichia coli -macrophage interaction

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