Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome.
<h4>Background</h4>Particulate matter (PM) is a key pollutant in ambient air that has been associated with negative health conditions in urban environments. The aim of this study was to examine the effects of orally administered PM on the gut microbiome and immune function under normal a...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/2fec43c10063492f956905991114cd82 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:2fec43c10063492f956905991114cd82 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:2fec43c10063492f956905991114cd822021-11-18T07:47:59ZEnvironmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome.1932-620310.1371/journal.pone.0062220https://doaj.org/article/2fec43c10063492f956905991114cd822013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638009/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Particulate matter (PM) is a key pollutant in ambient air that has been associated with negative health conditions in urban environments. The aim of this study was to examine the effects of orally administered PM on the gut microbiome and immune function under normal and inflammatory conditions.<h4>Methods</h4>Wild-type 129/SvEv mice were gavaged with Ottawa urban PM10 (EHC-93) for 7-14 days and mucosal gene expression analyzed using Ingenuity Pathways software. Intestinal permeability was measured by lactulose/mannitol excretion in urine. At sacrifice, segments of small and large intestine were cultured and cytokine secretion measured. Splenocytes were isolated and incubated with PM10 for measurement of proliferation. Long-term effects of exposure (35 days) on intestinal cytokine expression were measured in wild-type and IL-10 deficient (IL-10(-/-)) mice. Microbial composition of stool samples was assessed using terminal restriction fragment length polymorphism. Short chain fatty acids were measured in caecum.<h4>Results</h4>Short-term treatment of wild-type mice with PM10 altered immune gene expression, enhanced pro-inflammatory cytokine secretion in the small intestine, increased gut permeability, and induced hyporesponsiveness in splenocytes. Long-term treatment of wild-type and IL-10(-/-) mice increased pro-inflammatory cytokine expression in the colon and altered short chain fatty acid concentrations and microbial composition. IL-10(-/-) mice had increased disease as evidenced by enhanced histological damage.<h4>Conclusions</h4>Ingestion of airborne particulate matter alters the gut microbiome and induces acute and chronic inflammatory responses in the intestine.Lisa KishNaomi HotteGilaad G KaplanRenaud VincentRobert TsoMichael GänzleKevin P RiouxAducio ThiesenHerman W BarkemaEytan WineKaren L MadsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62220 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Lisa Kish Naomi Hotte Gilaad G Kaplan Renaud Vincent Robert Tso Michael Gänzle Kevin P Rioux Aducio Thiesen Herman W Barkema Eytan Wine Karen L Madsen Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. |
| description |
<h4>Background</h4>Particulate matter (PM) is a key pollutant in ambient air that has been associated with negative health conditions in urban environments. The aim of this study was to examine the effects of orally administered PM on the gut microbiome and immune function under normal and inflammatory conditions.<h4>Methods</h4>Wild-type 129/SvEv mice were gavaged with Ottawa urban PM10 (EHC-93) for 7-14 days and mucosal gene expression analyzed using Ingenuity Pathways software. Intestinal permeability was measured by lactulose/mannitol excretion in urine. At sacrifice, segments of small and large intestine were cultured and cytokine secretion measured. Splenocytes were isolated and incubated with PM10 for measurement of proliferation. Long-term effects of exposure (35 days) on intestinal cytokine expression were measured in wild-type and IL-10 deficient (IL-10(-/-)) mice. Microbial composition of stool samples was assessed using terminal restriction fragment length polymorphism. Short chain fatty acids were measured in caecum.<h4>Results</h4>Short-term treatment of wild-type mice with PM10 altered immune gene expression, enhanced pro-inflammatory cytokine secretion in the small intestine, increased gut permeability, and induced hyporesponsiveness in splenocytes. Long-term treatment of wild-type and IL-10(-/-) mice increased pro-inflammatory cytokine expression in the colon and altered short chain fatty acid concentrations and microbial composition. IL-10(-/-) mice had increased disease as evidenced by enhanced histological damage.<h4>Conclusions</h4>Ingestion of airborne particulate matter alters the gut microbiome and induces acute and chronic inflammatory responses in the intestine. |
| format |
article |
| author |
Lisa Kish Naomi Hotte Gilaad G Kaplan Renaud Vincent Robert Tso Michael Gänzle Kevin P Rioux Aducio Thiesen Herman W Barkema Eytan Wine Karen L Madsen |
| author_facet |
Lisa Kish Naomi Hotte Gilaad G Kaplan Renaud Vincent Robert Tso Michael Gänzle Kevin P Rioux Aducio Thiesen Herman W Barkema Eytan Wine Karen L Madsen |
| author_sort |
Lisa Kish |
| title |
Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. |
| title_short |
Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. |
| title_full |
Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. |
| title_fullStr |
Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. |
| title_full_unstemmed |
Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. |
| title_sort |
environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/2fec43c10063492f956905991114cd82 |
| work_keys_str_mv |
AT lisakish environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT naomihotte environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT gilaadgkaplan environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT renaudvincent environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT roberttso environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT michaelganzle environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT kevinprioux environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT aduciothiesen environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT hermanwbarkema environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT eytanwine environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome AT karenlmadsen environmentalparticulatematterinducesmurineintestinalinflammatoryresponsesandaltersthegutmicrobiome |
| _version_ |
1718422902535094272 |