Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.

Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports th...

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Autores principales:	Elvira Pelosi, Germana Castelli, Ines Martin-Padura, Veronica Bordoni, Simona Santoro, Alice Conigliaro, Anna Maria Cerio, Marco De Santis Puzzonia, Paola Marighetti, Mauro Biffoni, Tonino Alonzi, Laura Amicone, Myriam Alcalay, Francesco Bertolini, Ugo Testa, Marco Tripodi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2fefab18abe148faad5d7ff90302b185
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spelling	oai:doaj.org-article:2fefab18abe148faad5d7ff90302b1852021-11-18T08:06:24ZHuman haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.1932-620310.1371/journal.pone.0051109https://doaj.org/article/2fefab18abe148faad5d7ff90302b1852012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226561/?tool=EBIhttps://doaj.org/toc/1932-6203Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level.Elvira PelosiGermana CastelliInes Martin-PaduraVeronica BordoniSimona SantoroAlice ConigliaroAnna Maria CerioMarco De Santis PuzzoniaPaola MarighettiMauro BiffoniTonino AlonziLaura AmiconeMyriam AlcalayFrancesco BertoliniUgo TestaMarco TripodiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51109 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elvira Pelosi Germana Castelli Ines Martin-Padura Veronica Bordoni Simona Santoro Alice Conigliaro Anna Maria Cerio Marco De Santis Puzzonia Paola Marighetti Mauro Biffoni Tonino Alonzi Laura Amicone Myriam Alcalay Francesco Bertolini Ugo Testa Marco Tripodi Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
description	Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level.
format	article
author	Elvira Pelosi Germana Castelli Ines Martin-Padura Veronica Bordoni Simona Santoro Alice Conigliaro Anna Maria Cerio Marco De Santis Puzzonia Paola Marighetti Mauro Biffoni Tonino Alonzi Laura Amicone Myriam Alcalay Francesco Bertolini Ugo Testa Marco Tripodi
author_facet	Elvira Pelosi Germana Castelli Ines Martin-Padura Veronica Bordoni Simona Santoro Alice Conigliaro Anna Maria Cerio Marco De Santis Puzzonia Paola Marighetti Mauro Biffoni Tonino Alonzi Laura Amicone Myriam Alcalay Francesco Bertolini Ugo Testa Marco Tripodi
author_sort	Elvira Pelosi
title	Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
title_short	Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
title_full	Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
title_fullStr	Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
title_full_unstemmed	Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
title_sort	human haemato-endothelial precursors: cord blood cd34+ cells produce haemogenic endothelium.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2fefab18abe148faad5d7ff90302b185
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Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.

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