Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche

Abstract The failure of the osteosarcoma conventional therapies leads to the growing need for novel therapeutic strategies. The lack of specificity for the Cancer Stem Cells (CSCs) population has been recently identified as the main limitation in the current therapies. Moreover, the traditional two-...

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Autores principales:	Giada Bassi, Silvia Panseri, Samuele Maria Dozio, Monica Sandri, Elisabetta Campodoni, Massimiliano Dapporto, Simone Sprio, Anna Tampieri, Monica Montesi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ff1087cf6a24bccb16e8bbaf7023c1a
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spelling	oai:doaj.org-article:2ff1087cf6a24bccb16e8bbaf7023c1a2021-12-02T13:58:13ZScaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche10.1038/s41598-020-79448-y2045-2322https://doaj.org/article/2ff1087cf6a24bccb16e8bbaf7023c1a2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79448-yhttps://doaj.org/toc/2045-2322Abstract The failure of the osteosarcoma conventional therapies leads to the growing need for novel therapeutic strategies. The lack of specificity for the Cancer Stem Cells (CSCs) population has been recently identified as the main limitation in the current therapies. Moreover, the traditional two-dimensional (2D) in vitro models, employed in the drug testing and screening as well as in the study of cell and molecular biology, are affected by a poor in vitro-in vivo translation ability. To overcome these limitations, this work provides two tumour engineering approaches as new tools to address osteosarcoma and improve therapy outcomes. In detail, two different hydroxyapatite-based bone-mimicking scaffolds were used to recapitulate aspects of the in vivo tumour microenvironment, focusing on CSCs niche. The biological performance of human osteosarcoma cell lines (MG63 and SAOS-2) and enriched-CSCs were deeply analysed in these complex cell culture models. The results highlight the fundamental role of the tumour microenvironment proving the mimicry of osteosarcoma stem cell niche by the use of CSCs together with the biomimetic scaffolds, compared to conventional 2D culture systems. These advanced 3D cell culture in vitro tumour models could improve the predictivity of preclinical studies and strongly enhance the clinical translation.Giada BassiSilvia PanseriSamuele Maria DozioMonica SandriElisabetta CampodoniMassimiliano DapportoSimone SprioAnna TampieriMonica MontesiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giada Bassi Silvia Panseri Samuele Maria Dozio Monica Sandri Elisabetta Campodoni Massimiliano Dapporto Simone Sprio Anna Tampieri Monica Montesi Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche
description	Abstract The failure of the osteosarcoma conventional therapies leads to the growing need for novel therapeutic strategies. The lack of specificity for the Cancer Stem Cells (CSCs) population has been recently identified as the main limitation in the current therapies. Moreover, the traditional two-dimensional (2D) in vitro models, employed in the drug testing and screening as well as in the study of cell and molecular biology, are affected by a poor in vitro-in vivo translation ability. To overcome these limitations, this work provides two tumour engineering approaches as new tools to address osteosarcoma and improve therapy outcomes. In detail, two different hydroxyapatite-based bone-mimicking scaffolds were used to recapitulate aspects of the in vivo tumour microenvironment, focusing on CSCs niche. The biological performance of human osteosarcoma cell lines (MG63 and SAOS-2) and enriched-CSCs were deeply analysed in these complex cell culture models. The results highlight the fundamental role of the tumour microenvironment proving the mimicry of osteosarcoma stem cell niche by the use of CSCs together with the biomimetic scaffolds, compared to conventional 2D culture systems. These advanced 3D cell culture in vitro tumour models could improve the predictivity of preclinical studies and strongly enhance the clinical translation.
format	article
author	Giada Bassi Silvia Panseri Samuele Maria Dozio Monica Sandri Elisabetta Campodoni Massimiliano Dapporto Simone Sprio Anna Tampieri Monica Montesi
author_facet	Giada Bassi Silvia Panseri Samuele Maria Dozio Monica Sandri Elisabetta Campodoni Massimiliano Dapporto Simone Sprio Anna Tampieri Monica Montesi
author_sort	Giada Bassi
title	Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche
title_short	Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche
title_full	Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche
title_fullStr	Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche
title_full_unstemmed	Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche
title_sort	scaffold-based 3d cellular models mimicking the heterogeneity of osteosarcoma stem cell niche
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2ff1087cf6a24bccb16e8bbaf7023c1a
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Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche

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