Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)

Abstract Chinese mitten crab (Eriocheir sinensis) as a commercially important species is widely cultured in China. However, E. sinensis is prone to agonistic behavior, which causes physical damage and wastes energy resources, negatively impacting their growth and survival. Therefore, understanding t...

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Autores principales:	Yang-Yang Pang, Gen-Yong Huang, Ya-Meng Song, Xiao- Zhe Song, Jia-Huan Lv, Long He, Chao Niu, Ao-Ya Shi, Xing-Liang Shi, Yong-Xu Cheng, Xiao-Zhen Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ff344a3a04f4a8baa97febac2f417a6
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spelling	oai:doaj.org-article:2ff344a3a04f4a8baa97febac2f417a62021-12-02T13:20:02ZEffects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)10.1038/s41598-021-83984-62045-2322https://doaj.org/article/2ff344a3a04f4a8baa97febac2f417a62021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83984-6https://doaj.org/toc/2045-2322Abstract Chinese mitten crab (Eriocheir sinensis) as a commercially important species is widely cultured in China. However, E. sinensis is prone to agonistic behavior, which causes physical damage and wastes energy resources, negatively impacting their growth and survival. Therefore, understanding the regulatory mechanisms that underlie the switching of such behavior is essential for ensuring the efficient and cost-effective aquaculture of E. sinensis. The 5-HT2B receptor is a key downstream target of serotonin (5-HT), which is involved in regulating animal behavior. In this study, the full-length sequence of 5-HT2B gene was cloned. The total length of the 5-HT2B gene was found to be 3127 bp with a 236 bp 5′-UTR (untranslated region), a 779 bp 3′-UTR, and a 2112 bp open reading frame encoding 703 amino acids. Phylogenetic tree analysis revealed that the 5-HT2B amino acid sequence of E. sinensis is highly conserved with that of Cancer borealis. Using in vitro co-culture and luciferase assays, the miR-143 targets the 5-HT2B 3′-UTR and inhibits 5-HT2B expression was confirmed. Furthermore, RT-qPCR and Western blotting analyses revealed that the miR-143 mimic significantly inhibits 5-HT2B mRNA and protein expression. However, injection of miR-143 did not decrease agonistic behavior, indicating that 5-HT2B is not involved in the regulation of such behavior in E. sinensis.Yang-Yang PangGen-Yong HuangYa-Meng SongXiao- Zhe SongJia-Huan LvLong HeChao NiuAo-Ya ShiXing-Liang ShiYong-Xu ChengXiao-Zhen YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang-Yang Pang Gen-Yong Huang Ya-Meng Song Xiao- Zhe Song Jia-Huan Lv Long He Chao Niu Ao-Ya Shi Xing-Liang Shi Yong-Xu Cheng Xiao-Zhen Yang Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)
description	Abstract Chinese mitten crab (Eriocheir sinensis) as a commercially important species is widely cultured in China. However, E. sinensis is prone to agonistic behavior, which causes physical damage and wastes energy resources, negatively impacting their growth and survival. Therefore, understanding the regulatory mechanisms that underlie the switching of such behavior is essential for ensuring the efficient and cost-effective aquaculture of E. sinensis. The 5-HT2B receptor is a key downstream target of serotonin (5-HT), which is involved in regulating animal behavior. In this study, the full-length sequence of 5-HT2B gene was cloned. The total length of the 5-HT2B gene was found to be 3127 bp with a 236 bp 5′-UTR (untranslated region), a 779 bp 3′-UTR, and a 2112 bp open reading frame encoding 703 amino acids. Phylogenetic tree analysis revealed that the 5-HT2B amino acid sequence of E. sinensis is highly conserved with that of Cancer borealis. Using in vitro co-culture and luciferase assays, the miR-143 targets the 5-HT2B 3′-UTR and inhibits 5-HT2B expression was confirmed. Furthermore, RT-qPCR and Western blotting analyses revealed that the miR-143 mimic significantly inhibits 5-HT2B mRNA and protein expression. However, injection of miR-143 did not decrease agonistic behavior, indicating that 5-HT2B is not involved in the regulation of such behavior in E. sinensis.
format	article
author	Yang-Yang Pang Gen-Yong Huang Ya-Meng Song Xiao- Zhe Song Jia-Huan Lv Long He Chao Niu Ao-Ya Shi Xing-Liang Shi Yong-Xu Cheng Xiao-Zhen Yang
author_facet	Yang-Yang Pang Gen-Yong Huang Ya-Meng Song Xiao- Zhe Song Jia-Huan Lv Long He Chao Niu Ao-Ya Shi Xing-Liang Shi Yong-Xu Cheng Xiao-Zhen Yang
author_sort	Yang-Yang Pang
title	Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)
title_short	Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)
title_full	Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)
title_fullStr	Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)
title_full_unstemmed	Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)
title_sort	effects of mir-143 and its target receptor 5-ht2b on agonistic behavior in the chinese mitten crab (eriocheir sinensis)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2ff344a3a04f4a8baa97febac2f417a6
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Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)

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