Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress

Background: Cell toxicity due to diethanolamine (DEA) is well known but no data are available regarding its mechanism. The present study investigated the cell viability and proliferation ability of rat bone marrow mesenchymal stem cells (BMSCs) treated with DEA. Methods: At 3rd passages, BMSCs we...

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Autores principales:	Mohammad Hussein Abnosi, Setarehsadat Hosseini
Formato:	article
Lenguaje:	EN
Publicado:	Hamadan University of Medical Sciences 2020
Materias:	bone marrow mesenchymal stem cells di-ethanolamine cell viability cell proliferation oxidative stress Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/2ff527cbbfa9477fa46841a101273092
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spelling	oai:doaj.org-article:2ff527cbbfa9477fa46841a1012730922021-11-20T09:19:57ZDi-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress10.34172/ajmb.2020.052345-4113https://doaj.org/article/2ff527cbbfa9477fa46841a1012730922020-06-01T00:00:00Zhttp://ajmb.umsha.ac.ir/PDF/ajmb-8-35.pdfhttps://doaj.org/toc/2345-4113Background: Cell toxicity due to diethanolamine (DEA) is well known but no data are available regarding its mechanism. The present study investigated the cell viability and proliferation ability of rat bone marrow mesenchymal stem cells (BMSCs) treated with DEA. Methods: At 3rd passages, BMSCs were treated for 12, 24, and 48 hours with 0.025 to 16 mM of bis(2- ethylhexyl) phthalate. The cell viability was estimated using the trypan blue and MTT, then 1, 4, and 16 mM, and 48 hours were selected as well. Next, other parameters were determined, including proliferation ability, cell morphology, sodium and potassium levels, as well as the concentration of calcium, total protein, and the activity of metabolic enzymes (i.e., alanine transaminase [ALT], aspartate transaminase [AST], and lactate dehydrogenase [LDH]). Finally, malondialdehyde (MDA), total antioxidant capacity (TAC), and the activity of antioxidant enzymes such as superoxide dismutase and catalase were measured based on the aim of the study. Results: Based on the results, the viability reduced significantly from 0.6 mM at 12 hours and 0.2 for 24 and 48 hours (P<0.05). In addition, the proliferation ability showed a significant reduction (P<0.05) while the activity of LDH, ALT, and AST increased significantly (P<0.05). The level of electrolytes at 1 mM treatment demonstrated no change (P>0.05) whereas 4 mM concentration caused a decline in the calcium level while increased the sodium significantly (P<0.05). The results further revealed that the level of MDA increased although the activity of antioxidant enzymes and TAC represented a significant reduction (P<0.05). No change was detected in the morphology of nuclei while cytoplasm shrinkage and roundness were observable. Conclusion: In general, the findings of this study showed that DEA reduced the viability and proliferation of BMSCs via metabolic change, electrolyte imbalance, and the induction of oxidative stress. Mohammad Hussein Abnosi Setarehsadat HosseiniHamadan University of Medical Sciencesarticlebone marrow mesenchymal stem cellsdi-ethanolaminecell viabilitycell proliferationoxidative stressMedical technologyR855-855.5ENAvicenna Journal of Medical Biochemistry, Vol 8, Iss 1, Pp 35-43 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone marrow mesenchymal stem cells di-ethanolamine cell viability cell proliferation oxidative stress Medical technology R855-855.5
spellingShingle	bone marrow mesenchymal stem cells di-ethanolamine cell viability cell proliferation oxidative stress Medical technology R855-855.5 Mohammad Hussein Abnosi Setarehsadat Hosseini Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress
description	Background: Cell toxicity due to diethanolamine (DEA) is well known but no data are available regarding its mechanism. The present study investigated the cell viability and proliferation ability of rat bone marrow mesenchymal stem cells (BMSCs) treated with DEA. Methods: At 3rd passages, BMSCs were treated for 12, 24, and 48 hours with 0.025 to 16 mM of bis(2- ethylhexyl) phthalate. The cell viability was estimated using the trypan blue and MTT, then 1, 4, and 16 mM, and 48 hours were selected as well. Next, other parameters were determined, including proliferation ability, cell morphology, sodium and potassium levels, as well as the concentration of calcium, total protein, and the activity of metabolic enzymes (i.e., alanine transaminase [ALT], aspartate transaminase [AST], and lactate dehydrogenase [LDH]). Finally, malondialdehyde (MDA), total antioxidant capacity (TAC), and the activity of antioxidant enzymes such as superoxide dismutase and catalase were measured based on the aim of the study. Results: Based on the results, the viability reduced significantly from 0.6 mM at 12 hours and 0.2 for 24 and 48 hours (P<0.05). In addition, the proliferation ability showed a significant reduction (P<0.05) while the activity of LDH, ALT, and AST increased significantly (P<0.05). The level of electrolytes at 1 mM treatment demonstrated no change (P>0.05) whereas 4 mM concentration caused a decline in the calcium level while increased the sodium significantly (P<0.05). The results further revealed that the level of MDA increased although the activity of antioxidant enzymes and TAC represented a significant reduction (P<0.05). No change was detected in the morphology of nuclei while cytoplasm shrinkage and roundness were observable. Conclusion: In general, the findings of this study showed that DEA reduced the viability and proliferation of BMSCs via metabolic change, electrolyte imbalance, and the induction of oxidative stress.
format	article
author	Mohammad Hussein Abnosi Setarehsadat Hosseini
author_facet	Mohammad Hussein Abnosi Setarehsadat Hosseini
author_sort	Mohammad Hussein Abnosi
title	Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress
title_short	Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress
title_full	Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress
title_fullStr	Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress
title_full_unstemmed	Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress
title_sort	di-ethanolamine causes the mortality of bone marrow mesenchymal stem cell due to metabolic imbalance and oxidative stress
publisher	Hamadan University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/2ff527cbbfa9477fa46841a101273092
work_keys_str_mv	AT mohammadhusseinabnosi diethanolaminecausesthemortalityofbonemarrowmesenchymalstemcellduetometabolicimbalanceandoxidativestress AT setarehsadathosseini diethanolaminecausesthemortalityofbonemarrowmesenchymalstemcellduetometabolicimbalanceandoxidativestress
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Di-ethanolamine Causes the Mortality of Bone Marrow Mesenchymal Stem Cell Due to Metabolic Imbalance and Oxidative Stress

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