Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model

Abstract This paper presents two water-soluble fullerene nanomaterials (HexakisaminoC60 and monoglucosamineC60, which is called here JK39) that were developed and synthesized as non-viral siRNA transfection nanosystems. The developed two-step Bingel–Hirsch reaction enables the chemical modification...

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Autores principales:	Julia Korzuch, Monika Rak, Katarzyna Balin, Maciej Zubko, Olga Głowacka, Mateusz Dulski, Robert Musioł, Zbigniew Madeja, Maciej Serda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ff5f227aeb34945a7460424bb027439
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spelling	oai:doaj.org-article:2ff5f227aeb34945a7460424bb0274392021-12-02T16:51:04ZTowards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model10.1038/s41598-021-89943-52045-2322https://doaj.org/article/2ff5f227aeb34945a7460424bb0274392021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89943-5https://doaj.org/toc/2045-2322Abstract This paper presents two water-soluble fullerene nanomaterials (HexakisaminoC60 and monoglucosamineC60, which is called here JK39) that were developed and synthesized as non-viral siRNA transfection nanosystems. The developed two-step Bingel–Hirsch reaction enables the chemical modification of the fullerene scaffold with the desired bioactive fragments such as d-glucosamine while keeping the crucial positive charged ethylenediamine based malonate. The ESI–MS and 13C-NMR analyses of JK39 confirmed its high T h symmetry, while X-ray photoelectron spectroscopy revealed the presence of nitrogen and oxygen-containing C–O or C–N bonds. The efficiency of both fullerenes as siRNA vehicles was tested in vitro using the prostate cancer cell line DU145 expressing the GFP protein. The HexakisaminoC60 fullerene was an efficient siRNA transfection agent, and decreased the GFP fluorescence signal significantly in the DU145 cells. Surprisingly, the glycofullerene JK39 was inactive in the transfection experiments, probably due to its high zeta potential and the formation of an extremely stable complex with siRNA.Julia KorzuchMonika RakKatarzyna BalinMaciej ZubkoOlga GłowackaMateusz DulskiRobert MusiołZbigniew MadejaMaciej SerdaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julia Korzuch Monika Rak Katarzyna Balin Maciej Zubko Olga Głowacka Mateusz Dulski Robert Musioł Zbigniew Madeja Maciej Serda Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
description	Abstract This paper presents two water-soluble fullerene nanomaterials (HexakisaminoC60 and monoglucosamineC60, which is called here JK39) that were developed and synthesized as non-viral siRNA transfection nanosystems. The developed two-step Bingel–Hirsch reaction enables the chemical modification of the fullerene scaffold with the desired bioactive fragments such as d-glucosamine while keeping the crucial positive charged ethylenediamine based malonate. The ESI–MS and 13C-NMR analyses of JK39 confirmed its high T h symmetry, while X-ray photoelectron spectroscopy revealed the presence of nitrogen and oxygen-containing C–O or C–N bonds. The efficiency of both fullerenes as siRNA vehicles was tested in vitro using the prostate cancer cell line DU145 expressing the GFP protein. The HexakisaminoC60 fullerene was an efficient siRNA transfection agent, and decreased the GFP fluorescence signal significantly in the DU145 cells. Surprisingly, the glycofullerene JK39 was inactive in the transfection experiments, probably due to its high zeta potential and the formation of an extremely stable complex with siRNA.
format	article
author	Julia Korzuch Monika Rak Katarzyna Balin Maciej Zubko Olga Głowacka Mateusz Dulski Robert Musioł Zbigniew Madeja Maciej Serda
author_facet	Julia Korzuch Monika Rak Katarzyna Balin Maciej Zubko Olga Głowacka Mateusz Dulski Robert Musioł Zbigniew Madeja Maciej Serda
author_sort	Julia Korzuch
title	Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
title_short	Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
title_full	Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
title_fullStr	Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
title_full_unstemmed	Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model
title_sort	towards water-soluble [60]fullerenes for the delivery of sirna in a prostate cancer model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2ff5f227aeb34945a7460424bb027439
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Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model

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