Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection

Abstract Rapid and specific diagnostic techniques are needed to expedite specific treatment of bacterial skin infections with narrow-spectrum antibiotics, rather than broad-spectrum. Through this work a device was developed to determine the presence of and species responsible for a bacterial skin in...

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Autores principales:	Robin E. Sweeney, Elizabeth Budiman, Jeong-Yeol Yoon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ffc24fdbffe49a297cc82feb96bb86f
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spelling	oai:doaj.org-article:2ffc24fdbffe49a297cc82feb96bb86f2021-12-02T16:06:49ZMie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection10.1038/s41598-017-05061-12045-2322https://doaj.org/article/2ffc24fdbffe49a297cc82feb96bb86f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05061-1https://doaj.org/toc/2045-2322Abstract Rapid and specific diagnostic techniques are needed to expedite specific treatment of bacterial skin infections with narrow-spectrum antibiotics, rather than broad-spectrum. Through this work a device was developed to determine the presence of and species responsible for a bacterial skin infection using differences in Mie scatter spectra created by different bacterial species. A 650 nm LED at five different incident angles is used to illuminate the tissue, with Mie scatter being detected by PIN photodiodes at eight different detection angles. Mie scatter patterns are collected at all photodiode angles for each of the incident light angles, resulting in a Mie scatter spectra. Detectable differences in Mie scatter spectra were found using the device developed between commensal bacteria (no infection) and bacteria inoculated (infection) on the surface of both porcine and human cadaveric epidermis. Detectable differences were found between species of infection, specifically Escherichia coli and Staphylococcus aureus, with differences summarized through principle component analysis. Mie scatter spectra can be detected within a few seconds without skin contact. This device is the first to rapidly and specifically diagnose bacterial skin infections in a contact-less manner, allowing for initial treatment with narrow spectrum antibiotics, and helping to reduce the likelihood of resistance.Robin E. SweeneyElizabeth BudimanJeong-Yeol YoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robin E. Sweeney Elizabeth Budiman Jeong-Yeol Yoon Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection
description	Abstract Rapid and specific diagnostic techniques are needed to expedite specific treatment of bacterial skin infections with narrow-spectrum antibiotics, rather than broad-spectrum. Through this work a device was developed to determine the presence of and species responsible for a bacterial skin infection using differences in Mie scatter spectra created by different bacterial species. A 650 nm LED at five different incident angles is used to illuminate the tissue, with Mie scatter being detected by PIN photodiodes at eight different detection angles. Mie scatter patterns are collected at all photodiode angles for each of the incident light angles, resulting in a Mie scatter spectra. Detectable differences in Mie scatter spectra were found using the device developed between commensal bacteria (no infection) and bacteria inoculated (infection) on the surface of both porcine and human cadaveric epidermis. Detectable differences were found between species of infection, specifically Escherichia coli and Staphylococcus aureus, with differences summarized through principle component analysis. Mie scatter spectra can be detected within a few seconds without skin contact. This device is the first to rapidly and specifically diagnose bacterial skin infections in a contact-less manner, allowing for initial treatment with narrow spectrum antibiotics, and helping to reduce the likelihood of resistance.
format	article
author	Robin E. Sweeney Elizabeth Budiman Jeong-Yeol Yoon
author_facet	Robin E. Sweeney Elizabeth Budiman Jeong-Yeol Yoon
author_sort	Robin E. Sweeney
title	Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection
title_short	Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection
title_full	Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection
title_fullStr	Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection
title_full_unstemmed	Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection
title_sort	mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2ffc24fdbffe49a297cc82feb96bb86f
work_keys_str_mv	AT robinesweeney miescatterspectrabaseddeviceforinstantcontactfreeandspecificdiagnosisofbacterialskininfection AT elizabethbudiman miescatterspectrabaseddeviceforinstantcontactfreeandspecificdiagnosisofbacterialskininfection AT jeongyeolyoon miescatterspectrabaseddeviceforinstantcontactfreeandspecificdiagnosisofbacterialskininfection
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Mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection

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