Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan

Syeda Ayesha Ali,1,2 Muhammad Kamran Taj,1 Syeda Hafsa Ali3 1Center for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, Pakistan; 2Department of Biochemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan; 3Department of Microbiology,...

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Autores principales:	Ali SA, Taj MK, Ali SH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	bacterial meningitis cerebrospinal fluid 16s rrna incidence rate drug resistance Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/2ffec77b764047e4a869aa2bbb315158
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spelling	oai:doaj.org-article:2ffec77b764047e4a869aa2bbb3151582021-11-30T18:50:36ZAntimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan1178-6973https://doaj.org/article/2ffec77b764047e4a869aa2bbb3151582021-12-01T00:00:00Zhttps://www.dovepress.com/antimicrobial-resistance-pattern-of-bacterial-meningitis-among-patient-peer-reviewed-fulltext-article-IDRhttps://doaj.org/toc/1178-6973Syeda Ayesha Ali,1,2 Muhammad Kamran Taj,1 Syeda Hafsa Ali3 1Center for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, Pakistan; 2Department of Biochemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan; 3Department of Microbiology, Balochistan University of Information Technology, Engineering, and Management Sciences (BUITEMS), Quetta, PakistanCorrespondence: Muhammad Kamran TajCenter for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, 87300, PakistanEmail kamrancasvab@yahoo.comIntroduction: Bacterial meningitis (BM) is a lethal infectious disease that persists as a substantial cause of morbidity and mortality. The antimicrobial drug resistance in BM pathogens poses a major threat to the community while raising challenges for clinicians. For the first time, we elucidate the incidence rate of BM and its drug resistance among the population of Quetta, Balochistan.Methods: A cross-sectional study was conducted (from January 2018 to March 2021) among meningitis patients admitted to government hospitals in Quetta. The cerebrospinal fluid (CSF) specimen was collected and processed for microbiological and cytological analysis. Bacterial isolates were identified and confirmed using phylogenetic analysis of the 16S rRNA gene.Results: We found 35.9% (321/894) confirmed cases of BM among the population of Quetta, Balochistan. The identified bacterial isolates comprised Streptococcus pneumoniae 14.7% (130/894), Staphylococcus aureus 6.9% (61/894), Neisseria meningitidis 5.7% (51/894), Haemophilus influenzae 2.5% (22/894), Escherichia coli 4.5% (40/894), and Klebsiella pneumoniae 1.9% (17/894). A high percentage in male patients of 20.8% (186/894) was identified as compared to female patients of 15.1% (135/894). Extreme age groups such as infants (age range: 1 month to 1 year) and adults (age range: 61 years to 99 years) with low immunity were critically affected by BM. Clinical parameters such as abnormal CSF appearance, pH, and high WBCs in BM patients with endocarditis as the underlying disease were significantly at elevated risk of fatal outcome. 16S rRNA gene phylogenetic analysis confirmed the evolutionary similarity of isolated strains with prevailing pathogenic strains in Asia. Furthermore, age was identified as a significant risk factor for clinical outcomes.Conclusion: The majority of the identified BM patients belonged to rural areas with limited health-care facilities and paramedic staff. The high case fatality rate 11% demonstrates increased antibiotic resistance among BM isolates, thereby stimulating its devastation in underdeveloped regions. Timely detection and intensive treatment of BM may improve critical outcomes in patients.Keywords: bacterial meningitis, cerebrospinal fluid, 16S rRNA, incidence rate, drug resistanceAli SATaj MKAli SHDove Medical Pressarticlebacterial meningitiscerebrospinal fluid16s rrnaincidence ratedrug resistanceInfectious and parasitic diseasesRC109-216ENInfection and Drug Resistance, Vol Volume 14, Pp 5107-5120 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial meningitis cerebrospinal fluid 16s rrna incidence rate drug resistance Infectious and parasitic diseases RC109-216
spellingShingle	bacterial meningitis cerebrospinal fluid 16s rrna incidence rate drug resistance Infectious and parasitic diseases RC109-216 Ali SA Taj MK Ali SH Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan
description	Syeda Ayesha Ali,1,2 Muhammad Kamran Taj,1 Syeda Hafsa Ali3 1Center for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, Pakistan; 2Department of Biochemistry, Sardar Bahadur Khan Women’s University (SBKWU), Quetta, Pakistan; 3Department of Microbiology, Balochistan University of Information Technology, Engineering, and Management Sciences (BUITEMS), Quetta, PakistanCorrespondence: Muhammad Kamran TajCenter for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, 87300, PakistanEmail kamrancasvab@yahoo.comIntroduction: Bacterial meningitis (BM) is a lethal infectious disease that persists as a substantial cause of morbidity and mortality. The antimicrobial drug resistance in BM pathogens poses a major threat to the community while raising challenges for clinicians. For the first time, we elucidate the incidence rate of BM and its drug resistance among the population of Quetta, Balochistan.Methods: A cross-sectional study was conducted (from January 2018 to March 2021) among meningitis patients admitted to government hospitals in Quetta. The cerebrospinal fluid (CSF) specimen was collected and processed for microbiological and cytological analysis. Bacterial isolates were identified and confirmed using phylogenetic analysis of the 16S rRNA gene.Results: We found 35.9% (321/894) confirmed cases of BM among the population of Quetta, Balochistan. The identified bacterial isolates comprised Streptococcus pneumoniae 14.7% (130/894), Staphylococcus aureus 6.9% (61/894), Neisseria meningitidis 5.7% (51/894), Haemophilus influenzae 2.5% (22/894), Escherichia coli 4.5% (40/894), and Klebsiella pneumoniae 1.9% (17/894). A high percentage in male patients of 20.8% (186/894) was identified as compared to female patients of 15.1% (135/894). Extreme age groups such as infants (age range: 1 month to 1 year) and adults (age range: 61 years to 99 years) with low immunity were critically affected by BM. Clinical parameters such as abnormal CSF appearance, pH, and high WBCs in BM patients with endocarditis as the underlying disease were significantly at elevated risk of fatal outcome. 16S rRNA gene phylogenetic analysis confirmed the evolutionary similarity of isolated strains with prevailing pathogenic strains in Asia. Furthermore, age was identified as a significant risk factor for clinical outcomes.Conclusion: The majority of the identified BM patients belonged to rural areas with limited health-care facilities and paramedic staff. The high case fatality rate 11% demonstrates increased antibiotic resistance among BM isolates, thereby stimulating its devastation in underdeveloped regions. Timely detection and intensive treatment of BM may improve critical outcomes in patients.Keywords: bacterial meningitis, cerebrospinal fluid, 16S rRNA, incidence rate, drug resistance
format	article
author	Ali SA Taj MK Ali SH
author_facet	Ali SA Taj MK Ali SH
author_sort	Ali SA
title	Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan
title_short	Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan
title_full	Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan
title_fullStr	Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan
title_full_unstemmed	Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan
title_sort	antimicrobial resistance pattern of bacterial meningitis among patients in quetta, pakistan
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/2ffec77b764047e4a869aa2bbb315158
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Antimicrobial Resistance Pattern of Bacterial Meningitis Among Patients in Quetta, Pakistan

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