Anti-Hyperglycaemic Evaluation of Buddleia indica Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents

Buddleia indica Lam. is an ornamental evergreen shrub with few reports concerning its phytoconstituents and biological activities. Herein, the antihyperglycaemic activity of B. indica leaves methanol extract (BIT) was evaluated for the first time using in vitro...

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Autores principales:	Sameh S. Elhady, Fadia S. Youssef, Abdulrahman M. Alahdal, Diena M. Almasri, Mohamed L. Ashour
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	anti-hyperglycaemic activity <i>α</i>-amylase <i>Buddleia indica</i> <i>α</i>-glucosidase Scrophulariaceae streptozotocin-induced diabetes Botany QK1-989
Acceso en línea:	https://doaj.org/article/300158a962a443f0a3bb671a62bd8912
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spelling	oai:doaj.org-article:300158a962a443f0a3bb671a62bd89122021-11-25T18:45:41ZAnti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents10.3390/plants101123512223-7747https://doaj.org/article/300158a962a443f0a3bb671a62bd89122021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2351https://doaj.org/toc/2223-7747<i>Buddleia indica</i> Lam. is an ornamental evergreen shrub with few reports concerning its phytoconstituents and biological activities. Herein, the antihyperglycaemic activity of <i>B. indica</i> leaves methanol extract (BIT) was evaluated for the first time using in vitro and in vivo studies. Molecular modelling was performed for its major phytoconstituents that were further subjected to ADME/TOPAKT (absorption, distribution, metabolism, excretion and toxicity) prediction. BIT revealed considerable reduction in glucose concentration by 9.93% at 50 μg/mL using 3T3-L1 adipocyte culture. It displayed substantial inhibition versus <i>α</i>-glucosidase and <i>α</i>-amylase with IC<sub>50</sub> 205.2 and 385.06 μg/mL, respectively. In vivo antihyperglycaemic activity of BIT and the ethyl acetate fraction (BIE) was performed using streptozotocin-induced diabetes in rat model. BIT and BIE effectively ameliorate oxidative stress markers in addition to reducing serum blood glucose by 56.08 and 54.00%, respectively, and are associated with a substantial increase in serum insulin by 4.1 and 12.7%, respectively. This can be attributed to its richness with polyphenolic compounds comprising flavonoids, phenolic acids, phenyl propanoids and iridoids. Molecular docking showed that verbascoside and kaempferol displayed the highest fitting within human <i>α</i>-amylase (HA) and human <i>α</i>-glucosidase (HG) active sites, respectively. They showed reasonable pharmacokinetic, pharmacodynamic and toxicity properties, as revealed by ADME/TOPKAT study.Sameh S. ElhadyFadia S. YoussefAbdulrahman M. AlahdalDiena M. AlmasriMohamed L. AshourMDPI AGarticleanti-hyperglycaemic activity<i>α</i>-amylase<i>Buddleia indica</i><i>α</i>-glucosidaseScrophulariaceaestreptozotocin-induced diabetesBotanyQK1-989ENPlants, Vol 10, Iss 2351, p 2351 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-hyperglycaemic activity <i>α</i>-amylase <i>Buddleia indica</i> <i>α</i>-glucosidase Scrophulariaceae streptozotocin-induced diabetes Botany QK1-989
spellingShingle	anti-hyperglycaemic activity <i>α</i>-amylase <i>Buddleia indica</i> <i>α</i>-glucosidase Scrophulariaceae streptozotocin-induced diabetes Botany QK1-989 Sameh S. Elhady Fadia S. Youssef Abdulrahman M. Alahdal Diena M. Almasri Mohamed L. Ashour Anti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents
description	<i>Buddleia indica</i> Lam. is an ornamental evergreen shrub with few reports concerning its phytoconstituents and biological activities. Herein, the antihyperglycaemic activity of <i>B. indica</i> leaves methanol extract (BIT) was evaluated for the first time using in vitro and in vivo studies. Molecular modelling was performed for its major phytoconstituents that were further subjected to ADME/TOPAKT (absorption, distribution, metabolism, excretion and toxicity) prediction. BIT revealed considerable reduction in glucose concentration by 9.93% at 50 μg/mL using 3T3-L1 adipocyte culture. It displayed substantial inhibition versus <i>α</i>-glucosidase and <i>α</i>-amylase with IC<sub>50</sub> 205.2 and 385.06 μg/mL, respectively. In vivo antihyperglycaemic activity of BIT and the ethyl acetate fraction (BIE) was performed using streptozotocin-induced diabetes in rat model. BIT and BIE effectively ameliorate oxidative stress markers in addition to reducing serum blood glucose by 56.08 and 54.00%, respectively, and are associated with a substantial increase in serum insulin by 4.1 and 12.7%, respectively. This can be attributed to its richness with polyphenolic compounds comprising flavonoids, phenolic acids, phenyl propanoids and iridoids. Molecular docking showed that verbascoside and kaempferol displayed the highest fitting within human <i>α</i>-amylase (HA) and human <i>α</i>-glucosidase (HG) active sites, respectively. They showed reasonable pharmacokinetic, pharmacodynamic and toxicity properties, as revealed by ADME/TOPKAT study.
format	article
author	Sameh S. Elhady Fadia S. Youssef Abdulrahman M. Alahdal Diena M. Almasri Mohamed L. Ashour
author_facet	Sameh S. Elhady Fadia S. Youssef Abdulrahman M. Alahdal Diena M. Almasri Mohamed L. Ashour
author_sort	Sameh S. Elhady
title	Anti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents
title_short	Anti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents
title_full	Anti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents
title_fullStr	Anti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents
title_full_unstemmed	Anti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents
title_sort	anti-hyperglycaemic evaluation of <i>buddleia indica</i> leaves using in vitro, in vivo and in silico studies and its correlation with the major phytoconstituents
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/300158a962a443f0a3bb671a62bd8912
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Anti-Hyperglycaemic Evaluation of <i>Buddleia indica</i> Leaves Using In Vitro, In Vivo and In Silico Studies and Its Correlation with the Major Phytoconstituents

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