Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives

The analysis of viral nucleic acids (NA), DNA or RNA, is a crucial issue in the diagnosis of infections and the treatment and prevention of related human diseases. Conventional nucleic acid tests (NATs) require multistep approaches starting from the purification of the pathogen genetic material in b...

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Autores principales:	Emanuele Luigi Sciuto, Antonio Alessio Leonardi, Giovanna Calabrese, Giovanna De Luca, Maria Anna Coniglio, Alessia Irrera, Sabrina Conoci
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	infections diagnosis viral NA analysis point-of-care silicon-based technologies microfluidics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/30016ccd40d940ed9dc72e19e001cb80
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spelling	oai:doaj.org-article:30016ccd40d940ed9dc72e19e001cb802021-11-25T16:52:23ZNucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives10.3390/biom111115852218-273Xhttps://doaj.org/article/30016ccd40d940ed9dc72e19e001cb802021-10-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1585https://doaj.org/toc/2218-273XThe analysis of viral nucleic acids (NA), DNA or RNA, is a crucial issue in the diagnosis of infections and the treatment and prevention of related human diseases. Conventional nucleic acid tests (NATs) require multistep approaches starting from the purification of the pathogen genetic material in biological samples to the end of its detection, basically performed by the consolidated polymerase chain reaction (PCR), by the use of specialized instruments and dedicated laboratories. However, since the current NATs are too constraining and time and cost consuming, the research is evolving towards more integrated, decentralized, user-friendly, and low-cost methods. These will allow the implementation of massive diagnoses addressing the growing demand of fast and accurate viral analysis facing such global alerts as the pandemic of coronavirus disease of the recent period. Silicon-based technology and microfluidics, in this sense, brought an important step up, leading to the introduction of the genetic point-of-care (PoC) systems. This review goes through the evolution of the analytical methods for the viral NA diagnosis of infection diseases, highlighting both advantages and drawbacks of the innovative emerging technologies versus the conventional approaches.Emanuele Luigi SciutoAntonio Alessio LeonardiGiovanna CalabreseGiovanna De LucaMaria Anna ConiglioAlessia IrreraSabrina ConociMDPI AGarticleinfections diagnosisviral NA analysispoint-of-caresilicon-based technologiesmicrofluidicsMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1585, p 1585 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	infections diagnosis viral NA analysis point-of-care silicon-based technologies microfluidics Microbiology QR1-502
spellingShingle	infections diagnosis viral NA analysis point-of-care silicon-based technologies microfluidics Microbiology QR1-502 Emanuele Luigi Sciuto Antonio Alessio Leonardi Giovanna Calabrese Giovanna De Luca Maria Anna Coniglio Alessia Irrera Sabrina Conoci Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives
description	The analysis of viral nucleic acids (NA), DNA or RNA, is a crucial issue in the diagnosis of infections and the treatment and prevention of related human diseases. Conventional nucleic acid tests (NATs) require multistep approaches starting from the purification of the pathogen genetic material in biological samples to the end of its detection, basically performed by the consolidated polymerase chain reaction (PCR), by the use of specialized instruments and dedicated laboratories. However, since the current NATs are too constraining and time and cost consuming, the research is evolving towards more integrated, decentralized, user-friendly, and low-cost methods. These will allow the implementation of massive diagnoses addressing the growing demand of fast and accurate viral analysis facing such global alerts as the pandemic of coronavirus disease of the recent period. Silicon-based technology and microfluidics, in this sense, brought an important step up, leading to the introduction of the genetic point-of-care (PoC) systems. This review goes through the evolution of the analytical methods for the viral NA diagnosis of infection diseases, highlighting both advantages and drawbacks of the innovative emerging technologies versus the conventional approaches.
format	article
author	Emanuele Luigi Sciuto Antonio Alessio Leonardi Giovanna Calabrese Giovanna De Luca Maria Anna Coniglio Alessia Irrera Sabrina Conoci
author_facet	Emanuele Luigi Sciuto Antonio Alessio Leonardi Giovanna Calabrese Giovanna De Luca Maria Anna Coniglio Alessia Irrera Sabrina Conoci
author_sort	Emanuele Luigi Sciuto
title	Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives
title_short	Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives
title_full	Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives
title_fullStr	Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives
title_full_unstemmed	Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives
title_sort	nucleic acids analytical methods for viral infection diagnosis: state-of-the-art and future perspectives
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/30016ccd40d940ed9dc72e19e001cb80
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Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives

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