Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods

BACKGROUND AND OBJECTIVE: Caries is a multifactorial disease with a microbial nature. Thus, more concentration on its operative treatment will lead to failure in annihilating its origin. On the other hand, because of known side effects of antibiotics and world attraction to traditional treatment and...

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Autores principales:	H Kermanshah, S Hashemi Kamangar, S Arami, A Mirsalehian, M Kamalinegad, M Karimi, F Jabalameli
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2011
Materias:	dental caries streptococcus mutans lactobacillus rhamnosus actinomyces viscosus salvia officinalis mentha longifolia achillea millefolium pimpinella anisum antibacterial effect Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3002872ae871485db35b56f7f0a93320
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spelling	oai:doaj.org-article:3002872ae871485db35b56f7f0a933202021-11-10T08:56:58ZComparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods1561-41072251-7170https://doaj.org/article/3002872ae871485db35b56f7f0a933202011-11-01T00:00:00Zhttp://jbums.org/article-1-3921-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Caries is a multifactorial disease with a microbial nature. Thus, more concentration on its operative treatment will lead to failure in annihilating its origin. On the other hand, because of known side effects of antibiotics and world attraction to traditional treatment and importance of drug extraction of natural material and plants, this study was done to compare the antibacterial effect of hydroalcoholic extract of Salvia officinalis, Pimpinella anisum, Mentha longifolia and Achillea millefolium against Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces viscosus with two in vitro methods.METHODS: In this experimental study, hydroalcoholic extracts have been prepared from Salvia officinalis, Pimpinella anisum, Mentha longifolia and Achillea millefolium with maceration method. Their antibacterial activity against Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces viscosus have been evaluated with Broth macrodilution (the method in which amounts of antimicrobial agents is diluted in broth environment serially) (0.18-200 µg/ml) and Agar diffusion (the method in which antimicrobial effect is assessed in solid environment) (0.78-400 µg/ml) methods.FINDINGS: In Broth macrodilution method MIC (Minimum Inhibitory Concentration) for Pimpinella anisum, Salvia officinalis, Mentha longifolia and Achillea millefolium for Streptococcus mutans were respectively 12.5, 6.25, 12.5 and 50 µg/ml, for Lactobacillus rhamnosus 12.5,1.56, 3.12 and 12.5 µg/ml and for Actinomyces viscosus 50, 12.5, 100 and 50 µg/ml. In Agar diffusion method Pimpinella anisum and Salvia officinalis against Streptococcus mutans, Pimpinella anisum against Lactobacillus rhamnosus and Mentha longifolia against Actinomyces viscosus had antibacterial effect.CONCLUSION: According to the results of this study, four extracts had growth inhibitory effect on all three bacteria. Salvia officinalis had greater effect on inhibition of growth of all three bacteria. All of the extracts had bactericidal effect in the range of concentration.H KermanshahS Hashemi Kamangar,S AramiA Mirsalehian,M KamalinegadM Karimi,F JabalameliBabol University of Medical Sciencesarticledental cariesstreptococcus mutanslactobacillus rhamnosusactinomyces viscosussalvia officinalismentha longifoliaachillea millefoliumpimpinella anisumantibacterial effectMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 13, Iss 6, Pp 21-29 (2011)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	dental caries streptococcus mutans lactobacillus rhamnosus actinomyces viscosus salvia officinalis mentha longifolia achillea millefolium pimpinella anisum antibacterial effect Medicine R Medicine (General) R5-920
spellingShingle	dental caries streptococcus mutans lactobacillus rhamnosus actinomyces viscosus salvia officinalis mentha longifolia achillea millefolium pimpinella anisum antibacterial effect Medicine R Medicine (General) R5-920 H Kermanshah S Hashemi Kamangar, S Arami A Mirsalehian, M Kamalinegad M Karimi, F Jabalameli Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods
description	BACKGROUND AND OBJECTIVE: Caries is a multifactorial disease with a microbial nature. Thus, more concentration on its operative treatment will lead to failure in annihilating its origin. On the other hand, because of known side effects of antibiotics and world attraction to traditional treatment and importance of drug extraction of natural material and plants, this study was done to compare the antibacterial effect of hydroalcoholic extract of Salvia officinalis, Pimpinella anisum, Mentha longifolia and Achillea millefolium against Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces viscosus with two in vitro methods.METHODS: In this experimental study, hydroalcoholic extracts have been prepared from Salvia officinalis, Pimpinella anisum, Mentha longifolia and Achillea millefolium with maceration method. Their antibacterial activity against Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces viscosus have been evaluated with Broth macrodilution (the method in which amounts of antimicrobial agents is diluted in broth environment serially) (0.18-200 µg/ml) and Agar diffusion (the method in which antimicrobial effect is assessed in solid environment) (0.78-400 µg/ml) methods.FINDINGS: In Broth macrodilution method MIC (Minimum Inhibitory Concentration) for Pimpinella anisum, Salvia officinalis, Mentha longifolia and Achillea millefolium for Streptococcus mutans were respectively 12.5, 6.25, 12.5 and 50 µg/ml, for Lactobacillus rhamnosus 12.5,1.56, 3.12 and 12.5 µg/ml and for Actinomyces viscosus 50, 12.5, 100 and 50 µg/ml. In Agar diffusion method Pimpinella anisum and Salvia officinalis against Streptococcus mutans, Pimpinella anisum against Lactobacillus rhamnosus and Mentha longifolia against Actinomyces viscosus had antibacterial effect.CONCLUSION: According to the results of this study, four extracts had growth inhibitory effect on all three bacteria. Salvia officinalis had greater effect on inhibition of growth of all three bacteria. All of the extracts had bactericidal effect in the range of concentration.
format	article
author	H Kermanshah S Hashemi Kamangar, S Arami A Mirsalehian, M Kamalinegad M Karimi, F Jabalameli
author_facet	H Kermanshah S Hashemi Kamangar, S Arami A Mirsalehian, M Kamalinegad M Karimi, F Jabalameli
author_sort	H Kermanshah
title	Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods
title_short	Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods
title_full	Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods
title_fullStr	Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods
title_full_unstemmed	Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods
title_sort	comparison of antibacterial effect of hydroalcoholic extract of four plants against cariogenic microorganisms by two in vitro methods
publisher	Babol University of Medical Sciences
publishDate	2011
url	https://doaj.org/article/3002872ae871485db35b56f7f0a93320
work_keys_str_mv	AT hkermanshah comparisonofantibacterialeffectofhydroalcoholicextractoffourplantsagainstcariogenicmicroorganismsbytwoinvitromethods AT shashemikamangar comparisonofantibacterialeffectofhydroalcoholicextractoffourplantsagainstcariogenicmicroorganismsbytwoinvitromethods AT sarami comparisonofantibacterialeffectofhydroalcoholicextractoffourplantsagainstcariogenicmicroorganismsbytwoinvitromethods AT amirsalehian comparisonofantibacterialeffectofhydroalcoholicextractoffourplantsagainstcariogenicmicroorganismsbytwoinvitromethods AT mkamalinegad comparisonofantibacterialeffectofhydroalcoholicextractoffourplantsagainstcariogenicmicroorganismsbytwoinvitromethods AT mkarimi comparisonofantibacterialeffectofhydroalcoholicextractoffourplantsagainstcariogenicmicroorganismsbytwoinvitromethods AT fjabalameli comparisonofantibacterialeffectofhydroalcoholicextractoffourplantsagainstcariogenicmicroorganismsbytwoinvitromethods
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Comparison of Antibacterial Effect of Hydroalcoholic Extract of Four Plants against Cariogenic Microorganisms by two in Vitro Methods

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