Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort

Previous studies have reported abnormal muscle morphology and functions in patients with adolescent idiopathic scoliosis (AIS). To answer whether such abnormalities could be reflected in their circulation and their clinical implication for predicting curve progression to the surgical threshold, this...

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Autores principales:	Yujia Wang, Huanxiong Chen, Jiajun Zhang, Tsz-ping Lam, A.L.H. Hung, J.C.Y. Cheng, W.Y.W. Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	scoliosis iTRAQ α-actin progressive differentially expressed proteins Medicine R
Acceso en línea:	https://doaj.org/article/30041926c89848e68bc39a40ec1faa98
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spelling	oai:doaj.org-article:30041926c89848e68bc39a40ec1faa982021-11-11T17:33:55ZPotential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort10.3390/jcm102149272077-0383https://doaj.org/article/30041926c89848e68bc39a40ec1faa982021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/4927https://doaj.org/toc/2077-0383Previous studies have reported abnormal muscle morphology and functions in patients with adolescent idiopathic scoliosis (AIS). To answer whether such abnormalities could be reflected in their circulation and their clinical implication for predicting curve progression to the surgical threshold, this preliminary study explored the presence of baseline muscle-related proteins and their association with curve progression. Plasma samples were collected at the first clinical visit for AIS, with patients divided into non-progressive or progressive groups (<i>N</i> = four and four) according to their Cobb angle in six-year follow-ups, with age- and sex-matched healthy subjects (<i>N</i> = 50). Then, the samples were subjected to isobaric tags for relative and absolute quantitation (iTRAQ) for global comparison of untargeted protein expression. Seventy-one differentially expressed proteins (DEPs) were found elevated in progressive AIS. Functional analysis showed that 18 of these are expressed in muscles and play an essential role in muscle activities. Among the muscle-related DEPs, α-actin had the highest fold change in progressive/non-progressive groups. This preliminary study firstly suggested higher circulating levels of muscle structural proteins in progressive AIS, indicating the likelihood of structural damage at the microscopic level and its association with progression to the surgical threshold. Further studies with larger sample sizes are warranted to validate these novel candidates for early diagnosis and predicting progression.Yujia WangHuanxiong ChenJiajun ZhangTsz-ping LamA.L.H. HungJ.C.Y. ChengW.Y.W. LeeMDPI AGarticlescoliosisiTRAQα-actinprogressivedifferentially expressed proteinsMedicineRENJournal of Clinical Medicine, Vol 10, Iss 4927, p 4927 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	scoliosis iTRAQ α-actin progressive differentially expressed proteins Medicine R
spellingShingle	scoliosis iTRAQ α-actin progressive differentially expressed proteins Medicine R Yujia Wang Huanxiong Chen Jiajun Zhang Tsz-ping Lam A.L.H. Hung J.C.Y. Cheng W.Y.W. Lee Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort
description	Previous studies have reported abnormal muscle morphology and functions in patients with adolescent idiopathic scoliosis (AIS). To answer whether such abnormalities could be reflected in their circulation and their clinical implication for predicting curve progression to the surgical threshold, this preliminary study explored the presence of baseline muscle-related proteins and their association with curve progression. Plasma samples were collected at the first clinical visit for AIS, with patients divided into non-progressive or progressive groups (<i>N</i> = four and four) according to their Cobb angle in six-year follow-ups, with age- and sex-matched healthy subjects (<i>N</i> = 50). Then, the samples were subjected to isobaric tags for relative and absolute quantitation (iTRAQ) for global comparison of untargeted protein expression. Seventy-one differentially expressed proteins (DEPs) were found elevated in progressive AIS. Functional analysis showed that 18 of these are expressed in muscles and play an essential role in muscle activities. Among the muscle-related DEPs, α-actin had the highest fold change in progressive/non-progressive groups. This preliminary study firstly suggested higher circulating levels of muscle structural proteins in progressive AIS, indicating the likelihood of structural damage at the microscopic level and its association with progression to the surgical threshold. Further studies with larger sample sizes are warranted to validate these novel candidates for early diagnosis and predicting progression.
format	article
author	Yujia Wang Huanxiong Chen Jiajun Zhang Tsz-ping Lam A.L.H. Hung J.C.Y. Cheng W.Y.W. Lee
author_facet	Yujia Wang Huanxiong Chen Jiajun Zhang Tsz-ping Lam A.L.H. Hung J.C.Y. Cheng W.Y.W. Lee
author_sort	Yujia Wang
title	Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort
title_short	Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort
title_full	Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort
title_fullStr	Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort
title_full_unstemmed	Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort
title_sort	potential muscle-related biomarkers in predicting curve progression to the surgical threshold in adolescent idiopathic scoliosis—a pilot proteomic study comparing four non-progressive vs. four progressive patients vs. a control cohort
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/30041926c89848e68bc39a40ec1faa98
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Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis—A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort

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