Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes

The aim of the study was to determine the effect of probiotics, prebiotics and synbiotics administered in ovo on selected morphological parameters of the small intestine (duodenum, jejunum, ileum) in broiler chickens (Ross 308) and native chickens (Green-legged Partridge, GP). On the 12th day of emb...

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Autores principales:	Monika Bogusławska-Tryk, Ewa Ziółkowska, Anna Sławińska, Maria Siwek, Joanna Bogucka
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bioactive substances in ovo administration performance indices intestinal microstructure broiler chicken native chicken Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/3008890740a64799aec1d06d64ff53ea
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spelling	oai:doaj.org-article:3008890740a64799aec1d06d64ff53ea2021-11-25T16:20:41ZModulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes10.3390/ani111132932076-2615https://doaj.org/article/3008890740a64799aec1d06d64ff53ea2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3293https://doaj.org/toc/2076-2615The aim of the study was to determine the effect of probiotics, prebiotics and synbiotics administered in ovo on selected morphological parameters of the small intestine (duodenum, jejunum, ileum) in broiler chickens (Ross 308) and native chickens (Green-legged Partridge, GP). On the 12th day of embryonic development (the incubation period), an aqueous solution of a suitable bioactive substance was supplied in ovo to the egg’s air cell: probiotic—<i>Lactococcus lactis</i> subsp. <i>cremoris</i> (PRO), prebiotic—GOS, galacto-oligosaccharides (PRE) or symbiotic—GOS + <i>Lactococcus lactis</i> subsp. <i>cremoris</i> (SYN). Sterile saline was injected into control (CON) eggs. After hatching, the chicks were placed in pens (8 birds/pen, 4 replicates/group) and housed for 42 days. On the last day of the experiment, all birds were individually weighed and slaughtered. Samples for histological analysis were taken directly after slaughter from three sections of the small intestine. In samples from the duodenum, jejunum and ileum, the height and width of the intestinal villi (VH) were measured and their area (VA) was calculated, the depth of the intestinal crypts (CD) was determined, the thickness of the muscularis was measured and the ratio of the villus height to the crypt depth (V/C) was calculated. On the basis of the obtained data, it can be concluded that the applied substances administered in ovo affect the production parameters and intestinal morphology in broiler chickens and GP. The experiment showed a beneficial effect of in ovo stimulation with a prebiotic on the final body weight of Ross 308 compared to CON, while the effect of the administered substances on the intestinal microstructure is not unequivocal. In GP, the best effect in terms of villi height and V/C ratio was found in the in ovo synbiotic group. Taking into account the obtained results, it can be concluded that chickens of different genotypes react differently to a given substance; therefore, the substances should be adapted to the genotype.Monika Bogusławska-TrykEwa ZiółkowskaAnna SławińskaMaria SiwekJoanna BoguckaMDPI AGarticlebioactive substancesin ovo administrationperformance indicesintestinal microstructurebroiler chickennative chickenVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3293, p 3293 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioactive substances in ovo administration performance indices intestinal microstructure broiler chicken native chicken Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	bioactive substances in ovo administration performance indices intestinal microstructure broiler chicken native chicken Veterinary medicine SF600-1100 Zoology QL1-991 Monika Bogusławska-Tryk Ewa Ziółkowska Anna Sławińska Maria Siwek Joanna Bogucka Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes
description	The aim of the study was to determine the effect of probiotics, prebiotics and synbiotics administered in ovo on selected morphological parameters of the small intestine (duodenum, jejunum, ileum) in broiler chickens (Ross 308) and native chickens (Green-legged Partridge, GP). On the 12th day of embryonic development (the incubation period), an aqueous solution of a suitable bioactive substance was supplied in ovo to the egg’s air cell: probiotic—<i>Lactococcus lactis</i> subsp. <i>cremoris</i> (PRO), prebiotic—GOS, galacto-oligosaccharides (PRE) or symbiotic—GOS + <i>Lactococcus lactis</i> subsp. <i>cremoris</i> (SYN). Sterile saline was injected into control (CON) eggs. After hatching, the chicks were placed in pens (8 birds/pen, 4 replicates/group) and housed for 42 days. On the last day of the experiment, all birds were individually weighed and slaughtered. Samples for histological analysis were taken directly after slaughter from three sections of the small intestine. In samples from the duodenum, jejunum and ileum, the height and width of the intestinal villi (VH) were measured and their area (VA) was calculated, the depth of the intestinal crypts (CD) was determined, the thickness of the muscularis was measured and the ratio of the villus height to the crypt depth (V/C) was calculated. On the basis of the obtained data, it can be concluded that the applied substances administered in ovo affect the production parameters and intestinal morphology in broiler chickens and GP. The experiment showed a beneficial effect of in ovo stimulation with a prebiotic on the final body weight of Ross 308 compared to CON, while the effect of the administered substances on the intestinal microstructure is not unequivocal. In GP, the best effect in terms of villi height and V/C ratio was found in the in ovo synbiotic group. Taking into account the obtained results, it can be concluded that chickens of different genotypes react differently to a given substance; therefore, the substances should be adapted to the genotype.
format	article
author	Monika Bogusławska-Tryk Ewa Ziółkowska Anna Sławińska Maria Siwek Joanna Bogucka
author_facet	Monika Bogusławska-Tryk Ewa Ziółkowska Anna Sławińska Maria Siwek Joanna Bogucka
author_sort	Monika Bogusławska-Tryk
title	Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes
title_short	Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes
title_full	Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes
title_fullStr	Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes
title_full_unstemmed	Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes
title_sort	modulation of intestinal histology by probiotics, prebiotics and synbiotics delivered in ovo in distinct chicken genotypes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3008890740a64799aec1d06d64ff53ea
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Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes

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