Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.

<h4>Background</h4>A Chikungunya (CHIK) outbreak hit La Réunion Island in 2005-2006. The implicated vector was Aedes albopictus. Here, we present the first study on the susceptibility of Ae. albopictus populations to sympatric CHIKV isolates from La Réunion Island and compare it to other...

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Autores principales:	Marie Vazeille, Sara Moutailler, Daniel Coudrier, Claudine Rousseaux, Huot Khun, Michel Huerre, Julien Thiria, Jean-Sébastien Dehecq, Didier Fontenille, Isabelle Schuffenecker, Philippe Despres, Anna-Bella Failloux
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/300d842aab124a9b9801984a4d4c8236
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spelling	oai:doaj.org-article:300d842aab124a9b9801984a4d4c82362021-11-25T06:10:29ZTwo Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.1932-620310.1371/journal.pone.0001168https://doaj.org/article/300d842aab124a9b9801984a4d4c82362007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001168https://doaj.org/toc/1932-6203<h4>Background</h4>A Chikungunya (CHIK) outbreak hit La Réunion Island in 2005-2006. The implicated vector was Aedes albopictus. Here, we present the first study on the susceptibility of Ae. albopictus populations to sympatric CHIKV isolates from La Réunion Island and compare it to other virus/vector combinations.<h4>Methodology and findings</h4>We orally infected 8 Ae. albopictus collections from La Réunion and 3 from Mayotte collected in March 2006 with two Chikungunya virus (CHIKV) from La Réunion: (i) strain 05.115 collected in June 2005 with an Alanine at the position 226 of the glycoprotein E1 and (ii) strain 06.21 collected in November 2005 with a substitution A226V. Two other CHIKV isolates and four additional mosquito strains/species were also tested. The viral titer of the infectious blood-meal was 10(7) plaque forming units (pfu)/mL. Dissemination rates were assessed by immunofluorescent staining on head squashes of surviving females 14 days after infection. Rates were at least two times higher with CHIKV 06.21 compared to CHIKV 05.115. In addition, 10 individuals were analyzed every day by quantitative RT-PCR. Viral RNA was quantified on (i) whole females and (ii) midguts and salivary glands of infected females. When comparing profiles, CHIKV 06.21 produced nearly 2 log more viral RNA copies than CHIKV 05.115. Furthermore, females infected with CHIKV 05.115 could be divided in two categories: weakly susceptible or strongly susceptible, comparable to those infected by CHIKV 06.21. Histological analysis detected the presence of CHIKV in salivary glands two days after infection. In addition, Ae. albopictus from La Réunion was as efficient vector as Ae. aegypti and Ae. albopictus from Vietnam when infected with the CHIKV 06.21.<h4>Conclusions</h4>Our findings support the hypothesis that the CHIK outbreak in La Réunion Island was due to a highly competent vector Ae. albopictus which allowed an efficient replication and dissemination of CHIKV 06.21.Marie VazeilleSara MoutaillerDaniel CoudrierClaudine RousseauxHuot KhunMichel HuerreJulien ThiriaJean-Sébastien DehecqDidier FontenilleIsabelle SchuffeneckerPhilippe DespresAnna-Bella FaillouxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 11, p e1168 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie Vazeille Sara Moutailler Daniel Coudrier Claudine Rousseaux Huot Khun Michel Huerre Julien Thiria Jean-Sébastien Dehecq Didier Fontenille Isabelle Schuffenecker Philippe Despres Anna-Bella Failloux Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
description	<h4>Background</h4>A Chikungunya (CHIK) outbreak hit La Réunion Island in 2005-2006. The implicated vector was Aedes albopictus. Here, we present the first study on the susceptibility of Ae. albopictus populations to sympatric CHIKV isolates from La Réunion Island and compare it to other virus/vector combinations.<h4>Methodology and findings</h4>We orally infected 8 Ae. albopictus collections from La Réunion and 3 from Mayotte collected in March 2006 with two Chikungunya virus (CHIKV) from La Réunion: (i) strain 05.115 collected in June 2005 with an Alanine at the position 226 of the glycoprotein E1 and (ii) strain 06.21 collected in November 2005 with a substitution A226V. Two other CHIKV isolates and four additional mosquito strains/species were also tested. The viral titer of the infectious blood-meal was 10(7) plaque forming units (pfu)/mL. Dissemination rates were assessed by immunofluorescent staining on head squashes of surviving females 14 days after infection. Rates were at least two times higher with CHIKV 06.21 compared to CHIKV 05.115. In addition, 10 individuals were analyzed every day by quantitative RT-PCR. Viral RNA was quantified on (i) whole females and (ii) midguts and salivary glands of infected females. When comparing profiles, CHIKV 06.21 produced nearly 2 log more viral RNA copies than CHIKV 05.115. Furthermore, females infected with CHIKV 05.115 could be divided in two categories: weakly susceptible or strongly susceptible, comparable to those infected by CHIKV 06.21. Histological analysis detected the presence of CHIKV in salivary glands two days after infection. In addition, Ae. albopictus from La Réunion was as efficient vector as Ae. aegypti and Ae. albopictus from Vietnam when infected with the CHIKV 06.21.<h4>Conclusions</h4>Our findings support the hypothesis that the CHIK outbreak in La Réunion Island was due to a highly competent vector Ae. albopictus which allowed an efficient replication and dissemination of CHIKV 06.21.
format	article
author	Marie Vazeille Sara Moutailler Daniel Coudrier Claudine Rousseaux Huot Khun Michel Huerre Julien Thiria Jean-Sébastien Dehecq Didier Fontenille Isabelle Schuffenecker Philippe Despres Anna-Bella Failloux
author_facet	Marie Vazeille Sara Moutailler Daniel Coudrier Claudine Rousseaux Huot Khun Michel Huerre Julien Thiria Jean-Sébastien Dehecq Didier Fontenille Isabelle Schuffenecker Philippe Despres Anna-Bella Failloux
author_sort	Marie Vazeille
title	Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
title_short	Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
title_full	Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
title_fullStr	Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
title_full_unstemmed	Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.
title_sort	two chikungunya isolates from the outbreak of la reunion (indian ocean) exhibit different patterns of infection in the mosquito, aedes albopictus.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/300d842aab124a9b9801984a4d4c8236
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Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus.

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