Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.

Polyphagous pests cause significant economic loss worldwide through feeding damage on various cash crops. However, their diets in agricultural landscapes remain largely unexplored. Pest dietary evaluation in agricultural fields is a challenging task currently approached through visual observation of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Arash Kheirodin, Mohammad Sayari, Jason M Schmidt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/300e32f019bb40d88c218c1e0971acd4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:300e32f019bb40d88c218c1e0971acd4
record_format	dspace
spelling	oai:doaj.org-article:300e32f019bb40d88c218c1e0971acd42021-12-02T20:16:19ZRapid PCR-based method for herbivore dietary evaluation using plant-specific primers.1932-620310.1371/journal.pone.0260105https://doaj.org/article/300e32f019bb40d88c218c1e0971acd42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0260105https://doaj.org/toc/1932-6203Polyphagous pests cause significant economic loss worldwide through feeding damage on various cash crops. However, their diets in agricultural landscapes remain largely unexplored. Pest dietary evaluation in agricultural fields is a challenging task currently approached through visual observation of plant feeding and microscopic identification of semi-digested plant material in pest's guts. While molecular gut content analysis using metabarcoding approaches using universal primers (e.g., rbcl and trnL) have been successful in evaluating polyphagous pest diet, this method is relatively costly and time-consuming. Hence, there is a need for a rapid, specific, sensitive, and cost-effective method to screen for crops in the gut of pests. This is the first study to develop plant-specific primers that target various regions of their genomes, designed using a whole plant genome sequence. We selected Verticillium wilt disease resistance protein (VE-1) and pathogenesis related protein-coding genes 1-5 (PR-1-5) as our targets and designed species-specific primers for 14 important crops in the agroecosystems. Using amplicon sizes ranging from 115 to 407 bp, we developed two multiplex primer mixes that can separate nine and five plant species per PCR reaction, respectively. These two designed primer mixes provide a rapid, sensitive and specific route for polyphagous pest dietary evaluation in agroecosystems. This work will enable future research to rapidly expand our knowledge on the diet preference and range of crops that pests consume in various agroecosystems, which will help in the redesign and development of new crop rotation regimes to minimize polyphagous pest pressure and damage on crops.Arash KheirodinMohammad SayariJason M SchmidtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0260105 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arash Kheirodin Mohammad Sayari Jason M Schmidt Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.
description	Polyphagous pests cause significant economic loss worldwide through feeding damage on various cash crops. However, their diets in agricultural landscapes remain largely unexplored. Pest dietary evaluation in agricultural fields is a challenging task currently approached through visual observation of plant feeding and microscopic identification of semi-digested plant material in pest's guts. While molecular gut content analysis using metabarcoding approaches using universal primers (e.g., rbcl and trnL) have been successful in evaluating polyphagous pest diet, this method is relatively costly and time-consuming. Hence, there is a need for a rapid, specific, sensitive, and cost-effective method to screen for crops in the gut of pests. This is the first study to develop plant-specific primers that target various regions of their genomes, designed using a whole plant genome sequence. We selected Verticillium wilt disease resistance protein (VE-1) and pathogenesis related protein-coding genes 1-5 (PR-1-5) as our targets and designed species-specific primers for 14 important crops in the agroecosystems. Using amplicon sizes ranging from 115 to 407 bp, we developed two multiplex primer mixes that can separate nine and five plant species per PCR reaction, respectively. These two designed primer mixes provide a rapid, sensitive and specific route for polyphagous pest dietary evaluation in agroecosystems. This work will enable future research to rapidly expand our knowledge on the diet preference and range of crops that pests consume in various agroecosystems, which will help in the redesign and development of new crop rotation regimes to minimize polyphagous pest pressure and damage on crops.
format	article
author	Arash Kheirodin Mohammad Sayari Jason M Schmidt
author_facet	Arash Kheirodin Mohammad Sayari Jason M Schmidt
author_sort	Arash Kheirodin
title	Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.
title_short	Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.
title_full	Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.
title_fullStr	Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.
title_full_unstemmed	Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.
title_sort	rapid pcr-based method for herbivore dietary evaluation using plant-specific primers.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/300e32f019bb40d88c218c1e0971acd4
work_keys_str_mv	AT arashkheirodin rapidpcrbasedmethodforherbivoredietaryevaluationusingplantspecificprimers AT mohammadsayari rapidpcrbasedmethodforherbivoredietaryevaluationusingplantspecificprimers AT jasonmschmidt rapidpcrbasedmethodforherbivoredietaryevaluationusingplantspecificprimers
_version_	1718374516559708160

Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.

Ejemplares similares