Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease

Abstract In this study we used the Illumina Infinium Methylation array to investigate in a cohort of matched archival human tissue samples (n = 32) from 14 individuals with soft tissue sarcomas if genome-wide methylation changes occur during metastatic and recurrent (Met/Rec) disease. A range of sar...

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Autores principales:	Ana Cristina Vargas, Lesley-Ann Gray, Christine L. White, Fiona M. Maclean, Peter Grimison, Nima Mesbah Ardakani, Fiona Bonar, Elizabeth M. Algar, Alison L. Cheah, Peter Russell, Annabelle Mahar, Anthony J. Gill
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/301117d5d20546c08d54994327344cb2
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spelling	oai:doaj.org-article:301117d5d20546c08d54994327344cb22021-12-02T15:23:01ZGenome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease10.1038/s41598-020-79648-62045-2322https://doaj.org/article/301117d5d20546c08d54994327344cb22021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79648-6https://doaj.org/toc/2045-2322Abstract In this study we used the Illumina Infinium Methylation array to investigate in a cohort of matched archival human tissue samples (n = 32) from 14 individuals with soft tissue sarcomas if genome-wide methylation changes occur during metastatic and recurrent (Met/Rec) disease. A range of sarcoma types were selected for this study: leiomyosarcoma (LMS), myxofibrosarcoma (MFS), rhabdomyosarcoma (RMS) and synovial sarcoma (SS). We identified differential methylation in all Met/Rec matched samples, demonstrating that epigenomic differences develop during the clonal evolution of sarcomas. Differentially methylated regions and genes were detected, not been previously implicated in sarcoma progression, including at PTPRN2 and DAXX in LMS, WT1-AS and TNXB in SS, VENTX and NTRK3 in pleomorphic RMS and MEST and the C14MC / miR-379/miR-656 in MFS. Our overall findings indicate the presence of objective epigenetic differences across primary and Met/Rec human tissue samples not previously reported.Ana Cristina VargasLesley-Ann GrayChristine L. WhiteFiona M. MacleanPeter GrimisonNima Mesbah ArdakaniFiona BonarElizabeth M. AlgarAlison L. CheahPeter RussellAnnabelle MaharAnthony J. GillNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Cristina Vargas Lesley-Ann Gray Christine L. White Fiona M. Maclean Peter Grimison Nima Mesbah Ardakani Fiona Bonar Elizabeth M. Algar Alison L. Cheah Peter Russell Annabelle Mahar Anthony J. Gill Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease
description	Abstract In this study we used the Illumina Infinium Methylation array to investigate in a cohort of matched archival human tissue samples (n = 32) from 14 individuals with soft tissue sarcomas if genome-wide methylation changes occur during metastatic and recurrent (Met/Rec) disease. A range of sarcoma types were selected for this study: leiomyosarcoma (LMS), myxofibrosarcoma (MFS), rhabdomyosarcoma (RMS) and synovial sarcoma (SS). We identified differential methylation in all Met/Rec matched samples, demonstrating that epigenomic differences develop during the clonal evolution of sarcomas. Differentially methylated regions and genes were detected, not been previously implicated in sarcoma progression, including at PTPRN2 and DAXX in LMS, WT1-AS and TNXB in SS, VENTX and NTRK3 in pleomorphic RMS and MEST and the C14MC / miR-379/miR-656 in MFS. Our overall findings indicate the presence of objective epigenetic differences across primary and Met/Rec human tissue samples not previously reported.
format	article
author	Ana Cristina Vargas Lesley-Ann Gray Christine L. White Fiona M. Maclean Peter Grimison Nima Mesbah Ardakani Fiona Bonar Elizabeth M. Algar Alison L. Cheah Peter Russell Annabelle Mahar Anthony J. Gill
author_facet	Ana Cristina Vargas Lesley-Ann Gray Christine L. White Fiona M. Maclean Peter Grimison Nima Mesbah Ardakani Fiona Bonar Elizabeth M. Algar Alison L. Cheah Peter Russell Annabelle Mahar Anthony J. Gill
author_sort	Ana Cristina Vargas
title	Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease
title_short	Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease
title_full	Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease
title_fullStr	Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease
title_full_unstemmed	Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease
title_sort	genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/301117d5d20546c08d54994327344cb2
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Genome wide methylation profiling of selected matched soft tissue sarcomas identifies methylation changes in metastatic and recurrent disease

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