Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets

Abstract The application of phytases for animal feed in developing countries is limited due to the high cost of these enzymes, determined by the importation fees and the expensive substrates used for their production. In this work, we have used agroindustrial byproducts for the production of extract...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elizabeth Bárbara Epalanga Pires, Anderson Junior de Freitas, Fernanda França e Souza, Rafael Locatelli Salgado, Valéria Monteze Guimarães, Francisco Alves Pereira, Monique Renon Eller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/30165993c9d34de98ae1ace594a6ae5b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:30165993c9d34de98ae1ace594a6ae5b
record_format	dspace
spelling	oai:doaj.org-article:30165993c9d34de98ae1ace594a6ae5b2021-12-02T15:09:24ZProduction of Fungal Phytases from Agroindustrial Byproducts for Pig Diets10.1038/s41598-019-45720-z2045-2322https://doaj.org/article/30165993c9d34de98ae1ace594a6ae5b2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45720-zhttps://doaj.org/toc/2045-2322Abstract The application of phytases for animal feed in developing countries is limited due to the high cost of these enzymes, determined by the importation fees and the expensive substrates used for their production. In this work, we have used agroindustrial byproducts for the production of extracts containing phytases, which were accessed for their stability focusing on the conditions found in the gastrointestinal tract of pigs. The fungus Acremonim zeae presented higher phytase production in medium containing cornmeal, while the yeast Kluyveromyces marxianus produced 10-fold more phytase when cultivated on rice bran. Process optimization increased the difference in productivity to more than 300 fold. The phytase from A. zeae was thermostable, with higher activity at neutral pH and 50 °C, but was inhibited at pH 2.5 and by various ions. The phytase activity in the K. marxianus extract was stable at a wide range of conditions, which indicates the presence of at least two enzymes. As far as we know, this manuscript describes for the first time the phytase production and the characteristics of the extracts produced by both these microbial species. These enzymes could be produced at low cost and have potential to replace enzymes currently imported for this purpose.Elizabeth Bárbara Epalanga PiresAnderson Junior de FreitasFernanda França e SouzaRafael Locatelli SalgadoValéria Monteze GuimarãesFrancisco Alves PereiraMonique Renon EllerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elizabeth Bárbara Epalanga Pires Anderson Junior de Freitas Fernanda França e Souza Rafael Locatelli Salgado Valéria Monteze Guimarães Francisco Alves Pereira Monique Renon Eller Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets
description	Abstract The application of phytases for animal feed in developing countries is limited due to the high cost of these enzymes, determined by the importation fees and the expensive substrates used for their production. In this work, we have used agroindustrial byproducts for the production of extracts containing phytases, which were accessed for their stability focusing on the conditions found in the gastrointestinal tract of pigs. The fungus Acremonim zeae presented higher phytase production in medium containing cornmeal, while the yeast Kluyveromyces marxianus produced 10-fold more phytase when cultivated on rice bran. Process optimization increased the difference in productivity to more than 300 fold. The phytase from A. zeae was thermostable, with higher activity at neutral pH and 50 °C, but was inhibited at pH 2.5 and by various ions. The phytase activity in the K. marxianus extract was stable at a wide range of conditions, which indicates the presence of at least two enzymes. As far as we know, this manuscript describes for the first time the phytase production and the characteristics of the extracts produced by both these microbial species. These enzymes could be produced at low cost and have potential to replace enzymes currently imported for this purpose.
format	article
author	Elizabeth Bárbara Epalanga Pires Anderson Junior de Freitas Fernanda França e Souza Rafael Locatelli Salgado Valéria Monteze Guimarães Francisco Alves Pereira Monique Renon Eller
author_facet	Elizabeth Bárbara Epalanga Pires Anderson Junior de Freitas Fernanda França e Souza Rafael Locatelli Salgado Valéria Monteze Guimarães Francisco Alves Pereira Monique Renon Eller
author_sort	Elizabeth Bárbara Epalanga Pires
title	Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets
title_short	Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets
title_full	Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets
title_fullStr	Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets
title_full_unstemmed	Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets
title_sort	production of fungal phytases from agroindustrial byproducts for pig diets
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/30165993c9d34de98ae1ace594a6ae5b
work_keys_str_mv	AT elizabethbarbaraepalangapires productionoffungalphytasesfromagroindustrialbyproductsforpigdiets AT andersonjuniordefreitas productionoffungalphytasesfromagroindustrialbyproductsforpigdiets AT fernandafrancaesouza productionoffungalphytasesfromagroindustrialbyproductsforpigdiets AT rafaellocatellisalgado productionoffungalphytasesfromagroindustrialbyproductsforpigdiets AT valeriamontezeguimaraes productionoffungalphytasesfromagroindustrialbyproductsforpigdiets AT franciscoalvespereira productionoffungalphytasesfromagroindustrialbyproductsforpigdiets AT moniquerenoneller productionoffungalphytasesfromagroindustrialbyproductsforpigdiets
_version_	1718387805079470080

Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets

Ejemplares similares