Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals

Abstract With the development of massive parallel sequencing technology, it has become easier to establish new model organisms that are ideally suited to the specific biological phenomena of interest. Considering the history of research using classical model organisms, we believe that the efficient...

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Autores principales:	Yuji Suehiro, Sawako Yoshina, Tomoko Motohashi, Satoru Iwata, Katsufumi Dejima, Shohei Mitani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/301ba8ccca06423d805b46c450f7433e
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spelling	oai:doaj.org-article:301ba8ccca06423d805b46c450f7433e2021-12-02T14:17:16ZEfficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals10.1038/s41598-021-87226-72045-2322https://doaj.org/article/301ba8ccca06423d805b46c450f7433e2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87226-7https://doaj.org/toc/2045-2322Abstract With the development of massive parallel sequencing technology, it has become easier to establish new model organisms that are ideally suited to the specific biological phenomena of interest. Considering the history of research using classical model organisms, we believe that the efficient construction and sharing of gene mutation libraries will facilitate the progress of studies using these new model organisms. Using C. elegans, we applied the TMP/UV mutagenesis method to animals lacking function in the DNA damage response genes atm-1 and xpc-1. This method produces genetic mutations three times more efficiently than mutagenesis of wild-type animals. Furthermore, we confirmed that the use of next-generation sequencing and the elimination of false positives through machine learning could automate the process of mutation identification with an accuracy of over 95%. Eventually, we sequenced the whole genomes of 488 strains and isolated 981 novel mutations generated by the present method; these strains have been made available to anyone who wants to use them. Since the targeted DNA damage response genes are well conserved and the mutagens used in this study are also effective in a variety of species, we believe that our method is generally applicable to a wide range of animal species.Yuji SuehiroSawako YoshinaTomoko MotohashiSatoru IwataKatsufumi DejimaShohei MitaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuji Suehiro Sawako Yoshina Tomoko Motohashi Satoru Iwata Katsufumi Dejima Shohei Mitani Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals
description	Abstract With the development of massive parallel sequencing technology, it has become easier to establish new model organisms that are ideally suited to the specific biological phenomena of interest. Considering the history of research using classical model organisms, we believe that the efficient construction and sharing of gene mutation libraries will facilitate the progress of studies using these new model organisms. Using C. elegans, we applied the TMP/UV mutagenesis method to animals lacking function in the DNA damage response genes atm-1 and xpc-1. This method produces genetic mutations three times more efficiently than mutagenesis of wild-type animals. Furthermore, we confirmed that the use of next-generation sequencing and the elimination of false positives through machine learning could automate the process of mutation identification with an accuracy of over 95%. Eventually, we sequenced the whole genomes of 488 strains and isolated 981 novel mutations generated by the present method; these strains have been made available to anyone who wants to use them. Since the targeted DNA damage response genes are well conserved and the mutagens used in this study are also effective in a variety of species, we believe that our method is generally applicable to a wide range of animal species.
format	article
author	Yuji Suehiro Sawako Yoshina Tomoko Motohashi Satoru Iwata Katsufumi Dejima Shohei Mitani
author_facet	Yuji Suehiro Sawako Yoshina Tomoko Motohashi Satoru Iwata Katsufumi Dejima Shohei Mitani
author_sort	Yuji Suehiro
title	Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals
title_short	Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals
title_full	Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals
title_fullStr	Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals
title_full_unstemmed	Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals
title_sort	efficient collection of a large number of mutations by mutagenesis of dna damage response defective animals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/301ba8ccca06423d805b46c450f7433e
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Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals

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