Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells

Shaoling Wu,1 Xindong Zhao,2 Zhongguang Cui,1 Chunting Zhao,1 Yuzhen Wang,1 Li Du,1 Yanhui Li3 1Department of Hematology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, People's Republic of China; 2Department of Hematology, Medical College of Qingdao Univ...

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Autores principales:	Wu S, Zhao X, Cui Z, Zhao C, Wang Y, Du L, Li Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/302104e445644479a521984fdad75b4e
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spelling	oai:doaj.org-article:302104e445644479a521984fdad75b4e2021-12-02T07:21:42ZCytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells1178-2013https://doaj.org/article/302104e445644479a521984fdad75b4e2014-03-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-of-graphene-oxide-and-graphene-oxide-loaded-with-doxorubi-a16110https://doaj.org/toc/1178-2013 Shaoling Wu,1 Xindong Zhao,2 Zhongguang Cui,1 Chunting Zhao,1 Yuzhen Wang,1 Li Du,1 Yanhui Li3 1Department of Hematology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, People's Republic of China; 2Department of Hematology, Medical College of Qingdao University, Qingdao, Shandong, People's Republic of China; 3Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Qingdao, Shandong, People's Republic of China Abstract: The purpose of this study was to evaluate the cytotoxicity of human multiple myeloma cells (RPMI-8226) treated with graphene oxide (GO), doxorubicin (DOX), and GO loaded with DOX (GO/DOX). Cell viability was determined using the Cell Counting Kit-8 assay and analyzing the cell cycle and cell apoptosis. Cells treated with GO, GO/DOX, and pure DOX for 24 hours showed a decrease in proliferation. GO/DOX significantly inhibited cell proliferation as compared with pure DOX (P<0.01). When the effects of GO were removed, there was no observed difference between GO/DOX and pure DOX (P>0.05). Flow cytometry analysis of untreated and GO-, DOX-, and GO/DOX-treated cells found no significant differences in the G0/G1 phase (P>0.05), while significant differences were observed in the total apoptotic rates (P<0.05). No significant differences existed in the total apoptotic rates of GO-treated and untreated cells (P>0.05). These findings suggest that GO caused low cytotoxicity and did not induce cell apoptosis or change the cell cycle in multiple myeloma cells. Moreover, GO did not affect the antitumor activity of DOX. In conclusion, GO would be suitable as an anticancer drug nanocarrier and used to treat hematological malignancies. Keywords: graphene oxide, doxorubicin, human multiple myeloma cell, CCK-8, cell cycle, cell apoptosisWu SZhao XCui ZZhao CWang YDu LLi YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1413-1421 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wu S Zhao X Cui Z Zhao C Wang Y Du L Li Y Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells
description	Shaoling Wu,1 Xindong Zhao,2 Zhongguang Cui,1 Chunting Zhao,1 Yuzhen Wang,1 Li Du,1 Yanhui Li3 1Department of Hematology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, People's Republic of China; 2Department of Hematology, Medical College of Qingdao University, Qingdao, Shandong, People's Republic of China; 3Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Qingdao, Shandong, People's Republic of China Abstract: The purpose of this study was to evaluate the cytotoxicity of human multiple myeloma cells (RPMI-8226) treated with graphene oxide (GO), doxorubicin (DOX), and GO loaded with DOX (GO/DOX). Cell viability was determined using the Cell Counting Kit-8 assay and analyzing the cell cycle and cell apoptosis. Cells treated with GO, GO/DOX, and pure DOX for 24 hours showed a decrease in proliferation. GO/DOX significantly inhibited cell proliferation as compared with pure DOX (P<0.01). When the effects of GO were removed, there was no observed difference between GO/DOX and pure DOX (P>0.05). Flow cytometry analysis of untreated and GO-, DOX-, and GO/DOX-treated cells found no significant differences in the G0/G1 phase (P>0.05), while significant differences were observed in the total apoptotic rates (P<0.05). No significant differences existed in the total apoptotic rates of GO-treated and untreated cells (P>0.05). These findings suggest that GO caused low cytotoxicity and did not induce cell apoptosis or change the cell cycle in multiple myeloma cells. Moreover, GO did not affect the antitumor activity of DOX. In conclusion, GO would be suitable as an anticancer drug nanocarrier and used to treat hematological malignancies. Keywords: graphene oxide, doxorubicin, human multiple myeloma cell, CCK-8, cell cycle, cell apoptosis
format	article
author	Wu S Zhao X Cui Z Zhao C Wang Y Du L Li Y
author_facet	Wu S Zhao X Cui Z Zhao C Wang Y Du L Li Y
author_sort	Wu S
title	Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells
title_short	Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells
title_full	Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells
title_fullStr	Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells
title_full_unstemmed	Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells
title_sort	cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/302104e445644479a521984fdad75b4e
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Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells

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