Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.

The plant pathogen Pseudomonas syringae secretes multiple effectors that modulate plant defenses. Some effectors trigger defenses due to specific recognition by plant immune complexes, whereas others can suppress the resulting immune responses. The HopZ3 effector of P. syringae pv. syringae B728a (P...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Joanna Jeleńska, Jiyoung Lee, Andrew J Manning, Donald J Wolfgeher, Youngjoo Ahn, George Walters-Marrah, Ivan E Lopez, Lissette Garcia, Sheri A McClerklin, Richard W Michelmore, Stephen J Kron, Jean T Greenberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3028edd84eb74705ab7886f5ab35cdf3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3028edd84eb74705ab7886f5ab35cdf3
record_format	dspace
spelling	oai:doaj.org-article:3028edd84eb74705ab7886f5ab35cdf32021-12-02T19:59:54ZPseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.1553-73661553-737410.1371/journal.ppat.1010017https://doaj.org/article/3028edd84eb74705ab7886f5ab35cdf32021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1010017https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The plant pathogen Pseudomonas syringae secretes multiple effectors that modulate plant defenses. Some effectors trigger defenses due to specific recognition by plant immune complexes, whereas others can suppress the resulting immune responses. The HopZ3 effector of P. syringae pv. syringae B728a (PsyB728a) is an acetyltransferase that modifies not only components of plant immune complexes, but also the Psy effectors that activate these complexes. In Arabidopsis, HopZ3 acetylates the host RPM1 complex and the Psy effectors AvrRpm1 and AvrB3. This study focuses on the role of HopZ3 during tomato infection. In Psy-resistant tomato, the main immune complex includes PRF and PTO, a RIPK-family kinase that recognizes the AvrPto effector. HopZ3 acts as a virulence factor on tomato by suppressing AvrPto1Psy-triggered immunity. HopZ3 acetylates AvrPto1Psy and the host proteins PTO, SlRIPK and SlRIN4s. Biochemical reconstruction and site-directed mutagenesis experiments suggest that acetylation acts in multiple ways to suppress immune signaling in tomato. First, acetylation disrupts the critical AvrPto1Psy-PTO interaction needed to initiate the immune response. Unmodified residues at the binding interface of both proteins and at other residues needed for binding are acetylated. Second, acetylation occurs at residues important for AvrPto1Psy function but not for binding to PTO. Finally, acetylation reduces specific phosphorylations needed for promoting the immune-inducing activity of HopZ3's targets such as AvrPto1Psy and PTO. In some cases, acetylation competes with phosphorylation. HopZ3-mediated acetylation suppresses the kinase activity of SlRIPK and the phosphorylation of its SlRIN4 substrate previously implicated in PTO-signaling. Thus, HopZ3 disrupts the functions of multiple immune components and the effectors that trigger them, leading to increased susceptibility to infection. Finally, mass spectrometry used to map specific acetylated residues confirmed HopZ3's unusual capacity to modify histidine in addition to serine, threonine and lysine residues.Joanna JeleńskaJiyoung LeeAndrew J ManningDonald J WolfgeherYoungjoo AhnGeorge Walters-MarrahIvan E LopezLissette GarciaSheri A McClerklinRichard W MichelmoreStephen J KronJean T GreenbergPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 11, p e1010017 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Joanna Jeleńska Jiyoung Lee Andrew J Manning Donald J Wolfgeher Youngjoo Ahn George Walters-Marrah Ivan E Lopez Lissette Garcia Sheri A McClerklin Richard W Michelmore Stephen J Kron Jean T Greenberg Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.
description	The plant pathogen Pseudomonas syringae secretes multiple effectors that modulate plant defenses. Some effectors trigger defenses due to specific recognition by plant immune complexes, whereas others can suppress the resulting immune responses. The HopZ3 effector of P. syringae pv. syringae B728a (PsyB728a) is an acetyltransferase that modifies not only components of plant immune complexes, but also the Psy effectors that activate these complexes. In Arabidopsis, HopZ3 acetylates the host RPM1 complex and the Psy effectors AvrRpm1 and AvrB3. This study focuses on the role of HopZ3 during tomato infection. In Psy-resistant tomato, the main immune complex includes PRF and PTO, a RIPK-family kinase that recognizes the AvrPto effector. HopZ3 acts as a virulence factor on tomato by suppressing AvrPto1Psy-triggered immunity. HopZ3 acetylates AvrPto1Psy and the host proteins PTO, SlRIPK and SlRIN4s. Biochemical reconstruction and site-directed mutagenesis experiments suggest that acetylation acts in multiple ways to suppress immune signaling in tomato. First, acetylation disrupts the critical AvrPto1Psy-PTO interaction needed to initiate the immune response. Unmodified residues at the binding interface of both proteins and at other residues needed for binding are acetylated. Second, acetylation occurs at residues important for AvrPto1Psy function but not for binding to PTO. Finally, acetylation reduces specific phosphorylations needed for promoting the immune-inducing activity of HopZ3's targets such as AvrPto1Psy and PTO. In some cases, acetylation competes with phosphorylation. HopZ3-mediated acetylation suppresses the kinase activity of SlRIPK and the phosphorylation of its SlRIN4 substrate previously implicated in PTO-signaling. Thus, HopZ3 disrupts the functions of multiple immune components and the effectors that trigger them, leading to increased susceptibility to infection. Finally, mass spectrometry used to map specific acetylated residues confirmed HopZ3's unusual capacity to modify histidine in addition to serine, threonine and lysine residues.
format	article
author	Joanna Jeleńska Jiyoung Lee Andrew J Manning Donald J Wolfgeher Youngjoo Ahn George Walters-Marrah Ivan E Lopez Lissette Garcia Sheri A McClerklin Richard W Michelmore Stephen J Kron Jean T Greenberg
author_facet	Joanna Jeleńska Jiyoung Lee Andrew J Manning Donald J Wolfgeher Youngjoo Ahn George Walters-Marrah Ivan E Lopez Lissette Garcia Sheri A McClerklin Richard W Michelmore Stephen J Kron Jean T Greenberg
author_sort	Joanna Jeleńska
title	Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.
title_short	Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.
title_full	Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.
title_fullStr	Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.
title_full_unstemmed	Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.
title_sort	pseudomonas syringae effector hopz3 suppresses the bacterial avrpto1-tomato pto immune complex via acetylation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3028edd84eb74705ab7886f5ab35cdf3
work_keys_str_mv	AT joannajelenska pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT jiyounglee pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT andrewjmanning pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT donaldjwolfgeher pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT youngjooahn pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT georgewaltersmarrah pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT ivanelopez pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT lissettegarcia pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT sheriamcclerklin pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT richardwmichelmore pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT stephenjkron pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation AT jeantgreenberg pseudomonassyringaeeffectorhopz3suppressesthebacterialavrpto1tomatoptoimmunecomplexviaacetylation
_version_	1718375712440713216

Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation.

Ejemplares similares