Pooled library screening with multiplexed Cpf1 library

AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.

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Autores principales:	Jintan Liu, Sanjana Srinivasan, Chieh-Yuan Li, I-Lin Ho, Johnathon Rose, MennatAllah Shaheen, Gang Wang, Wantong Yao, Angela Deem, Chris Bristow, Traver Hart, Giulio Draetta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/302d4902b46145a7a871a5d3d6846970
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spelling	oai:doaj.org-article:302d4902b46145a7a871a5d3d68469702021-12-02T15:35:31ZPooled library screening with multiplexed Cpf1 library10.1038/s41467-019-10963-x2041-1723https://doaj.org/article/302d4902b46145a7a871a5d3d68469702019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10963-xhttps://doaj.org/toc/2041-1723AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.Jintan LiuSanjana SrinivasanChieh-Yuan LiI-Lin HoJohnathon RoseMennatAllah ShaheenGang WangWantong YaoAngela DeemChris BristowTraver HartGiulio DraettaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jintan Liu Sanjana Srinivasan Chieh-Yuan Li I-Lin Ho Johnathon Rose MennatAllah Shaheen Gang Wang Wantong Yao Angela Deem Chris Bristow Traver Hart Giulio Draetta Pooled library screening with multiplexed Cpf1 library
description	AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.
format	article
author	Jintan Liu Sanjana Srinivasan Chieh-Yuan Li I-Lin Ho Johnathon Rose MennatAllah Shaheen Gang Wang Wantong Yao Angela Deem Chris Bristow Traver Hart Giulio Draetta
author_facet	Jintan Liu Sanjana Srinivasan Chieh-Yuan Li I-Lin Ho Johnathon Rose MennatAllah Shaheen Gang Wang Wantong Yao Angela Deem Chris Bristow Traver Hart Giulio Draetta
author_sort	Jintan Liu
title	Pooled library screening with multiplexed Cpf1 library
title_short	Pooled library screening with multiplexed Cpf1 library
title_full	Pooled library screening with multiplexed Cpf1 library
title_fullStr	Pooled library screening with multiplexed Cpf1 library
title_full_unstemmed	Pooled library screening with multiplexed Cpf1 library
title_sort	pooled library screening with multiplexed cpf1 library
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/302d4902b46145a7a871a5d3d6846970
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Pooled library screening with multiplexed Cpf1 library

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