Detection of C-Peptide in Scalp Hair of Healthy Adults

Darya S Abdulateef,1 Jamal M Salih1,2 1Physiology Department, College of Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq; 2Diabetes Physician, Diabetes and Endocrine Centre, Sulaymaniyah, Kurdistan Region, IraqCorrespondence: Darya S AbdulateefPhysiology Department, College o...

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Autores principales:	Abdulateef DS, Salih JM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	c-peptide hair sample fasting plasma glucose human. Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/303436be8c53437ba3d947f59357ab41
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spelling	oai:doaj.org-article:303436be8c53437ba3d947f59357ab412021-12-02T05:27:10ZDetection of C-Peptide in Scalp Hair of Healthy Adults1178-7007https://doaj.org/article/303436be8c53437ba3d947f59357ab412020-02-01T00:00:00Zhttps://www.dovepress.com/detection-of-c-peptide-in-scalp-hair-of-healthy-adults-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Darya S Abdulateef,1 Jamal M Salih1,2 1Physiology Department, College of Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq; 2Diabetes Physician, Diabetes and Endocrine Centre, Sulaymaniyah, Kurdistan Region, IraqCorrespondence: Darya S AbdulateefPhysiology Department, College of Medicine, University of Sulaimani, New–Street-27, Zone 209, P.O. Box: 334, Sulaymaniyah, Kurdistan Region, IraqTel +964-7701580313Email darya.abdulateef@univsul.edu.iqPurpose: The C-peptide level from blood and urine samples represents endogenous insulin secretion and has clinical implications for individuals with and without diabetes. However, the study results are inconsistent and the available methods can only measure short-term C-peptide levels, which can be substantially affected by concurrent glycaemia. In this study, we evaluated whether C-peptide can be detected from hair? Hair C-peptide if detected could potentially represent long-term, insulin secretion. And to know whether there is any correlation between hair C-peptide with certain biochemical and demographic parameters.Methods: In a prospective observational study on 120 normal healthy individuals, hair samples were taken from the scalp and C-peptide was extracted. The hair C-peptide levels were measured in pg/mg of hair using electrochemiluminescence immunoassay (ECLISA) after methanol extraction and were compared among different age and body-type groups. Serum C-peptide, fasting plasma glucose (FPG) and HbA1c were assessed and their levels were correlated with anthropometric parameters.Results: A detectable range of C-peptide was found in the hair samples of all the subjects with a median of 63.59 pg/mg hair and it was positively correlated with FPG but no significant correlation was found between hair C-peptide and serum C-peptide levels. The hair C-peptide level, along with FPG and HbA1c, was significantly different according to age.Conclusion: These results conclude that C-peptide can be detected from and measured in the scalp hair of humans and it is positively correlated with FPG, and it is significantly different according to age. This technique, if validated, may verify its usefulness in future studies of both healthy and diseased subjects.Keywords: C-peptide, hair sample, fasting plasma glucose, humanAbdulateef DSSalih JMDove Medical Pressarticlec-peptidehair samplefasting plasma glucosehuman.Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 227-236 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	c-peptide hair sample fasting plasma glucose human. Specialties of internal medicine RC581-951
spellingShingle	c-peptide hair sample fasting plasma glucose human. Specialties of internal medicine RC581-951 Abdulateef DS Salih JM Detection of C-Peptide in Scalp Hair of Healthy Adults
description	Darya S Abdulateef,1 Jamal M Salih1,2 1Physiology Department, College of Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq; 2Diabetes Physician, Diabetes and Endocrine Centre, Sulaymaniyah, Kurdistan Region, IraqCorrespondence: Darya S AbdulateefPhysiology Department, College of Medicine, University of Sulaimani, New–Street-27, Zone 209, P.O. Box: 334, Sulaymaniyah, Kurdistan Region, IraqTel +964-7701580313Email darya.abdulateef@univsul.edu.iqPurpose: The C-peptide level from blood and urine samples represents endogenous insulin secretion and has clinical implications for individuals with and without diabetes. However, the study results are inconsistent and the available methods can only measure short-term C-peptide levels, which can be substantially affected by concurrent glycaemia. In this study, we evaluated whether C-peptide can be detected from hair? Hair C-peptide if detected could potentially represent long-term, insulin secretion. And to know whether there is any correlation between hair C-peptide with certain biochemical and demographic parameters.Methods: In a prospective observational study on 120 normal healthy individuals, hair samples were taken from the scalp and C-peptide was extracted. The hair C-peptide levels were measured in pg/mg of hair using electrochemiluminescence immunoassay (ECLISA) after methanol extraction and were compared among different age and body-type groups. Serum C-peptide, fasting plasma glucose (FPG) and HbA1c were assessed and their levels were correlated with anthropometric parameters.Results: A detectable range of C-peptide was found in the hair samples of all the subjects with a median of 63.59 pg/mg hair and it was positively correlated with FPG but no significant correlation was found between hair C-peptide and serum C-peptide levels. The hair C-peptide level, along with FPG and HbA1c, was significantly different according to age.Conclusion: These results conclude that C-peptide can be detected from and measured in the scalp hair of humans and it is positively correlated with FPG, and it is significantly different according to age. This technique, if validated, may verify its usefulness in future studies of both healthy and diseased subjects.Keywords: C-peptide, hair sample, fasting plasma glucose, human
format	article
author	Abdulateef DS Salih JM
author_facet	Abdulateef DS Salih JM
author_sort	Abdulateef DS
title	Detection of C-Peptide in Scalp Hair of Healthy Adults
title_short	Detection of C-Peptide in Scalp Hair of Healthy Adults
title_full	Detection of C-Peptide in Scalp Hair of Healthy Adults
title_fullStr	Detection of C-Peptide in Scalp Hair of Healthy Adults
title_full_unstemmed	Detection of C-Peptide in Scalp Hair of Healthy Adults
title_sort	detection of c-peptide in scalp hair of healthy adults
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/303436be8c53437ba3d947f59357ab41
work_keys_str_mv	AT abdulateefds detectionofcpeptideinscalphairofhealthyadults AT salihjm detectionofcpeptideinscalphairofhealthyadults
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Detection of C-Peptide in Scalp Hair of Healthy Adults

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